Fast visualization of internal structure of multiple-scattering objects by diffusion optical tomography

2002 ◽  
Author(s):  
Vladimir V. Shuvalov ◽  
I. V. Shutov ◽  
E. V. Tret'akov
2000 ◽  
Vol 30 (1) ◽  
pp. 78-80 ◽  
Author(s):  
E V Malikov ◽  
V M Petnikova ◽  
D A Chursin ◽  
Vladimir V Shuvalov ◽  
I V Shutov

2019 ◽  
Vol 126 (3) ◽  
pp. 226-231 ◽  
Author(s):  
G. G. Levin ◽  
V. L. Minaev ◽  
K. N. Min’kov ◽  
M. M. Ermakov ◽  
A. A. Samoilenko

2012 ◽  
Vol 113 (6) ◽  
pp. 975-983 ◽  
Author(s):  
Manuela Kellner ◽  
Marko Heidrich ◽  
Rebecca Beigel ◽  
Raoul-Amadeus Lorbeer ◽  
Lars Knudsen ◽  
...  

The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.


Author(s):  
Morteza Hasani Shoreh ◽  
Alexandre Goy ◽  
JooWon Lim ◽  
Ulugbek Kamilov ◽  
Michael Unser ◽  
...  

Author(s):  
S. Golladay

The theory of multiple scattering has been worked out by Groves and comparisons have been made between predicted and observed signals for thick specimens observed in a STEM under conditions where phase contrast effects are unimportant. Independent measurements of the collection efficiencies of the two STEM detectors, calculations of the ratio σe/σi = R, where σe, σi are the total cross sections for elastic and inelastic scattering respectively, and a model of the unknown mass distribution are needed for these comparisons. In this paper an extension of this work will be described which allows the determination of the required efficiencies, R, and the unknown mass distribution from the data without additional measurements or models. Essential to the analysis is the fact that in a STEM two or more signal measurements can be made simultaneously at each image point.


Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.


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