Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages

2002 ◽  
Author(s):  
Chaoyang Xu ◽  
Shici Tan ◽  
Da Xing
Keyword(s):  
Low Power ◽  
Intracellular Calcium ◽  
Laser Irradiation ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Cellular Activity ◽  
Hene Laser ◽  
Mouse Macrophages

scholarly journals Calhex231 Ameliorates Cardiac Hypertrophy by Inhibiting Cellular Autophagy in Vivo and in Vitro

2015 ◽  
Vol 36 (4) ◽  
pp. 1597-1612 ◽  
Author(s):  
Lei Liu ◽  
Chao Wang ◽  
Dianjun Sun ◽  
Shuangquan Jiang ◽  
Hong Li ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cardiac Hypertrophy ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Heart Weight ◽  
Cardiomyocyte Hypertrophy ◽  
Sham Operation ◽  
Ang Ii

Background/Aims: Intracellular calcium concentration ([Ca2+]i) homeostasis, an initial factor of cardiac hypertrophy, is regulated by the calcium-sensing receptor (CaSR) and is associated with the formation of autolysosomes. The aim of this study was to investigate the role of Calhex231, a CaSR inhibitor, on the hypertrophic response via autophagy modulation. Methods: Cardiac hypertrophy was induced by transverse aortic constriction (TAC) in 40 male Wistar rats, while 10 rats underwent a sham operation and served as controls. Cardiac function was monitored by transthoracic echocardiography, and the hypertrophy index was calculated. Cardiac tissue was stained with hematoxylin and eosin (H&E) or Masson's trichrome reagent and examined by transmission electron microscopy. An angiotensin II (Ang II)-induced cardiomyocyte hypertrophy model was established and used to test the involvement of active molecules. Intracellular calcium concentration ([Ca2+]i) was determined by the introduction of Fluo-4/AM dye followed by confocal microscopy. The expression of various active proteins was analyzed by western blot. Results: The rats with TAC-induced hypertrophy had an increased heart size, ratio of heart weight to body weight, myocardial fibrosis, and CaSR and autophagy levels, which were suppressed by Calhex231. Experimental results using Ang II-induced hypertrophic cardiomyocytes confirmed that Calhex231 suppressed CaSR expression and downregulated autophagy by inhibiting the Ca2+/calmodulin-dependent-protein kinase-kinase-β (CaMKKβ)- AMP-activated protein kinase (AMPK)-mammalian target of rapamycin (mTOR) pathway to ameliorate cardiomyocyte hypertrophy. Conclusions: Calhex231 ameliorates myocardial hypertrophy induced by pressure-overload or Ang II via inhibiting CaSR expression and autophagy. Our results may support the notion that Calhex231 can become a new therapeutic agent for the treatment of cardiac hypertrophy.

Changes of intracellular calcium concentration at in vitro fertilization of bovine oocytes

1994 ◽  
Vol 41 (1) ◽  
pp. 259 ◽  
Author(s):  
J. Mizuno ◽  
K. Nakada ◽  
K. Endo ◽  
K. Kobayashi ◽  
Y. Nishikata ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Vitro Fertilization ◽  
Bovine Oocytes

scholarly journals Simultaneous arterial calcium dynamics and diameter measurements: application to myoendothelial communication

2001 ◽  
Vol 280 (3) ◽  
pp. H1088-H1096 ◽  
Author(s):  
Alexander Schuster ◽  
Hirotaka Oishi ◽  
Jean-Louis Bény ◽  
Nikolaos Stergiopulos ◽  
Jean-Jacques Meister
Keyword(s):  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Palmitoleic Acid ◽  
Intracellular Calcium Concentration ◽  
Calcium Flux ◽  
Arterial Segment ◽  
Free Intracellular Calcium ◽  
Image Acquisition System ◽  
Stimulation Of

The goal of the present study was to analyze the intercellular calcium communication between smooth muscle cells (SMCs) and endothelial cells (ECs) by simultaneously monitoring artery diameter and intracellular calcium concentration in a rat mesenteric arterial segment in vitro under physiological pressure (50 mmHg) and flow (50 μl/min) in a specially developed system. Intracellular calcium was expressed as the fura 2 ratio. The diameter was measured using a digital image acquisition system. Stimulation of SMCs with the α1-agonist phenylephrine (PE) caused not only an increase in the free intracellular calcium concentration of the SMCs as expected but also in the ECs, suggesting a calcium flux from the SMCs to the ECs. The gap junction uncoupler palmitoleic acid greatly reduced this increase in calcium in the ECs on stimulation of the SMCs with PE. This indicates that the signaling pathway passes through the gap junctions. Similarly, although vasomotion originates in the SMCs, calcium oscillates in both SMCs and ECs during vasomotion, suggesting again a calcium flux from the SMCs to the ECs.

Substance P induces a rise in intracellular calcium concentration in human T lymphocytes in vitro: evidence of a receptor-independent mechanism

1993 ◽  
Vol 42 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Annemieke Kavelaars ◽  
Frank Jeurissen ◽  
Jacobien von Frijtag Drabbe Künzel ◽  
J.Herman van Roijen ◽  
Ger T. Rijkers ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Substance P ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Intracellular Calcium Concentration ◽  
Human T Lymphocytes ◽  
Independent Mechanism
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