Spectral investigation of normal skin tissue in vivo via fiber-optical evanescent wave Fourier transform infrared (FEW-FTIR) spectroscopy

2001 ◽  
Author(s):  
Angelique Kano
Keyword(s):  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Evanescent Wave ◽  
Normal Skin ◽  
Fourier Transform Infrared ◽  
Spectral Investigation ◽  
Skin Tissue ◽  
Fiber Optical

Investigations of normal skin tissue using fiber optical FTIR spectroscopy

1998 ◽  
Author(s):  
Angelique L. Brooks ◽  
Reinhard F. Bruch ◽  
Natalia I. Afanasyeva ◽  
Sergei F. Kolyakov ◽  
Leonid N. Butvina ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Normal Skin ◽  
Skin Tissue ◽  
Fiber Optical

scholarly journals Cell apoptosis specific marker found by Fourier Transform Infrared Spectroscopy

2004 ◽  
Vol 18 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Silvia Gaudenzi ◽  
Deleana Pozzi ◽  
Paolo Toro ◽  
Ida Silvestri ◽  
Stefania Morrone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Ph Value ◽  
Fourier Transform Infrared ◽  
Specific Marker ◽  
Chemical Agent

We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm−1and 2800 cm−1, a “marker band” of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non‒destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologiesin vivo.

Detection of bloodstains using attenuated total reflectance-Fourier transform infrared spectroscopy supported with PCA and PCA–LDA

2021 ◽  
pp. 002580242110109
Author(s):  
Sweety Sharma ◽  
Rito Chophi ◽  
Jaskirandeep Kaur Jossan ◽  
Rajinder Singh
Keyword(s):  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Body Fluid ◽  
Due Process ◽  
Dna Analysis ◽  
Fourier Transform Infrared ◽  
Body Fluids ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Non Destructive

The most important task in a criminal investigation is to detect and identify the recovered biological stains beyond reasonable scientific doubt and preserve the sample for further DNA analysis. In the light of this fact, many presumptive and confirmatory tests are routinely employed in the forensic laboratories to determine the type of body fluid. However, the currently used techniques are specific to one type of body fluid and hence it cannot be utilized to differentiate multiple body fluids. Moreover, these tests consume the samples in due process, and thus it becomes a great limitation especially considering the fact that samples are recovered in minute quantity in forensic cases. Therefore, such limitations necessitate the use of non-destructive techniques that can be applied simultaneously to all types of bodily fluids and allow sample preservation for further analysis. In the current work, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy has been used to circumvent the aforementioned limitations. The important factors which could influence the detection of blood such as the effect of substrates, washing/chemical treatment, ageing, and dilution limits on the analysis of blood have been analysed. In addition, blood discrimination from non-blood substance (biological and non-biological in nature) has also been studied. Chemometric technique that is PCA–LDA has been used to discriminate blood from other body fluids and it resulted in 100% accurate classification. Furthermore, blood and non-blood substances including fake blood have also been classified into separate clusters with a 100% accuracy, sensitivity, and specificity. All-inclusive, this preliminary study substantiates the potential application of ATR-FTIR spectroscopy for the non-destructive identification of blood traces in simulated forensic casework conditions with 0% rate of false classification.

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