Novel high-resolution large-field scan-and-repeat projection lithography system

1991 ◽  
Author(s):  
Kanti Jain
Keyword(s):  
High Resolution ◽  
Large Field ◽  
Projection Lithography ◽  
Lithography System

scholarly journals Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dwaipayan Adhya ◽  
George Chennell ◽  
James A. Crowe ◽  
Eva P. Valencia-Alarcón ◽  
James Seyforth ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Light Sheet ◽  
Autism Spectrum ◽  
Model Systems ◽  
Large Field ◽  
Autism Spectrum Conditions ◽  
Patient Specific ◽  
Airy Beam

Abstract Background The inability to observe relevant biological processes in vivo significantly restricts human neurodevelopmental research. Advances in appropriate in vitro model systems, including patient-specific human brain organoids and human cortical spheroids (hCSs), offer a pragmatic solution to this issue. In particular, hCSs are an accessible method for generating homogenous organoids of dorsal telencephalic fate, which recapitulate key aspects of human corticogenesis, including the formation of neural rosettes—in vitro correlates of the neural tube. These neurogenic niches give rise to neural progenitors that subsequently differentiate into neurons. Studies differentiating induced pluripotent stem cells (hiPSCs) in 2D have linked atypical formation of neural rosettes with neurodevelopmental disorders such as autism spectrum conditions. Thus far, however, conventional methods of tissue preparation in this field limit the ability to image these structures in three-dimensions within intact hCS or other 3D preparations. To overcome this limitation, we have sought to optimise a methodological approach to process hCSs to maximise the utility of a novel Airy-beam light sheet microscope (ALSM) to acquire high resolution volumetric images of internal structures within hCS representative of early developmental time points. Results Conventional approaches to imaging hCS by confocal microscopy were limited in their ability to image effectively into intact spheroids. Conversely, volumetric acquisition by ALSM offered superior imaging through intact, non-clarified, in vitro tissues, in both speed and resolution when compared to conventional confocal imaging systems. Furthermore, optimised immunohistochemistry and optical clearing of hCSs afforded improved imaging at depth. This permitted visualization of the morphology of the inner lumen of neural rosettes. Conclusion We present an optimized methodology that takes advantage of an ALSM system that can rapidly image intact 3D brain organoids at high resolution while retaining a large field of view. This imaging modality can be applied to both non-cleared and cleared in vitro human brain spheroids derived from hiPSCs for precise examination of their internal 3D structures. This process represents a rapid, highly efficient method to examine and quantify in 3D the formation of key structures required for the coordination of neurodevelopmental processes in both health and disease states. We posit that this approach would facilitate investigation of human neurodevelopmental processes in vitro.

Solid-state VCSEL beam scanner with ultra-large field of view and high resolution

2021 ◽  
Author(s):  
Ruixiao Li ◽  
Zeuku Ho ◽  
Xiaodong Gu ◽  
Satoshi Shinada ◽  
Fumio Koyama
Keyword(s):  
Solid State ◽  
High Resolution ◽  
Field Of View ◽  
Large Field

The Best of Both Worlds: 3D X-ray Microscopy with Ultra-high Resolution and a Large Field of View

2011 ◽  
Author(s):  
W. Li ◽  
J. Gelb ◽  
Y. Yang ◽  
Y. Guan ◽  
W. Wu ◽  
...  
Keyword(s):  
High Resolution ◽  
Field Of View ◽  
Large Field ◽  
X Ray

Influence of electron acceleration voltage in the cell-projection lithography system

2000 ◽  
Vol 53 (1-4) ◽  
pp. 353-356
Author(s):  
M. Kotera ◽  
K. Yamaguchi ◽  
M. Sakai ◽  
K. Naruse ◽  
T. Okagawa ◽  
...  
Keyword(s):  
Electron Acceleration ◽  
Projection Lithography ◽  
Acceleration Voltage ◽  
Lithography System

scholarly journals Shadow monochromatic backlighting: Large-field high resolution X-ray shadowgraphy with improved spectral tunability

2001 ◽  
Vol 19 (2) ◽  
pp. 285-293 ◽  
Author(s):  
T.A. PIKUZ ◽  
A. YA. FAENOV ◽  
M. FRAENKEL ◽  
A. ZIGLER ◽  
F. FLORA ◽  
...  
Keyword(s):  
High Resolution ◽  
High Spatial Resolution ◽  
Field Of View ◽  
Large Field ◽  
X Ray ◽  
Traditional Scheme ◽  
Wide Range ◽  
Wide Wavelength Range ◽  
Bent Crystals ◽  
First Time

The shadow monochromatic backlighting (SMB) scheme, a modification of the well-known soft X-ray monochromatic backlighting scheme, is proposed. It is based on a spherical crystal as the dispersive element and extends the traditional scheme by allowing one to work with a wide range of Bragg angles and thus in a wide spectral range. The advantages of the new scheme are demonstrated experimentally and supported numerically by ray-tracing simulations. In the experiments, the X-ray backlighter source is a laser-produced plasma, created by the interaction of an ultrashort pulse, Ti:Sapphire laser (120 fs, 3–5 mJ, 1016 W/cm2 on target) or a short wavelength XeCl laser (10 ns, 1–2 J, 1013 W/cm2 on target) with various solid targets (Dy, Ni + Cr, BaF2). In both experiments, the X-ray sources are well localized spatially (∼20 μm) and are spectrally tunable in a relatively wide wavelength range (λ = 8–15 Å). High quality monochromatic (δλ/λ ∼ 10−5–10−3) images with high spatial resolution (up to ∼4 μm) over a large field of view (a few square millimeters) were obtained. Utilization of spherically bent crystals to obtain high-resolution, large field, monochromatic images in a wide range of Bragg angles (35° < Θ < 90°) is demonstrated for the first time.

Introduction to the Six Eye Movement Systems and the Visual Fixation System

2008 ◽  
Author(s):  
Agnes Wong
Keyword(s):  
Visual Acuity ◽  
Eye Movements ◽  
High Resolution ◽  
Information Overload ◽  
Survival Rates ◽  
Large Field ◽  
Field Of Vision ◽  
Very High Spatial Resolution ◽  
Different Characteristics ◽  
The Brain

One main reason that we make eye movements is to solve a problem of information overload. A large field of vision allows an animal to survey the environment for food and to avoid predators, thus increasing its survival rate. Similarly, a high visual acuity also increases survival rates by allowing an animal to aim at a target more accurately, leading to higher killing rates and more food. However, there are simply not enough neurons in the brain to support a visual system that has high resolution over the entire field of vision. Faced with the competing evolutionary demands for high visual acuity and a large field of vision, an effective strategy is needed so that the brain will not be overwhelmed by a large amount of visual input. Some animals, such as rabbits, give up high resolution in favor of a larger field of vision (rabbits can see nearly 360°), whereas others, such as hawks, restrict their field of vision in return for a high visual acuity (hawks have vision as good as 20/2, about 10 times better than humans). In humans, rather than using one strategy over the other, the retina develops a very high spatial resolution in the center (i.e., the fovea), and a much lower resolution in the periphery. Although this “foveal compromise” strategy solves the problem of information overload, one result is that unless the image of an object of interest happens to fall on the fovea, the image is relegated to the low-resolution retinal periphery. The evolution of a mechanism to move the eyes is therefore necessary to complement this foveal compromise strategy by ensuring that an object of interest is maintained or brought to the fovea. To maintain the image of an object on the fovea, the vestibulo-ocular (VOR) and optokinetic systems generate eye movements to compensate for head motions. Likewise, the saccadic, smooth pursuit, and vergence systems generate eye movements to bring the image of an object of interest on the fovea. These different eye movements have different characteristics and involve different parts of the brain.

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