Structural and thermal modeling of a cooled CCD camera

2001 ◽  
Author(s):  
Anees Ahmad ◽  
Thomas D. Arndt ◽  
Robert Gross ◽  
Mark Hahn ◽  
Mark Panasiti
Keyword(s):  
Thermal Modeling ◽  
Ccd Camera ◽  
Cooled Ccd

Five-Dimensional Microscopy using Widefield-Deconvolution: Practical Considerations and Biological Applications

1996 ◽  
Vol 54 ◽  
pp. 268-269
Author(s):  
W.F. Marshall ◽  
K. Oegema ◽  
J. Nunnari ◽  
A.F. Straight ◽  
D.A. Agard ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
High Speed ◽  
Laser Scanning ◽  
Ccd Camera ◽  
Image Plane ◽  
Time Lapse ◽  
Laser Scanning Confocal Microscopy ◽  
Three Dimensions ◽  
Excitation Light ◽  
Cooled Ccd

The ability to image cells in three dimensions has brought about a revolution in biological microscopy, enabling many questions to be asked which would be inaccessible without this capability. There are currently two major methods of three dimensional microscopy: laser-scanning confocal microscopy and widefield-deconvolution microscopy. The method of widefield-deconvolution uses a cooled CCD to acquire images from a standard widefield microscope, and then computationally removes out of focus blur. Using such a scheme, it is easy to acquire time-lapse 3D images of living cells without killing them, and to do so for multiple wavelengths (using computer-controlled filter wheels). Thus, it is now not only feasible, but routine, to perform five dimensional microscopy (three spatial dimensions, plus time, plus wavelength).Widefield-deconvolution has several advantages over confocal microscopy. The two main advantages are high speed of acquisition (because there is no scanning, a single optical section is acquired at a time by using a cooled CCD camera) and the use of low excitation light levels Excitation intensity can be much lower than in a confocal microscope for three reasons: 1) longer exposures can be taken since the entire 512x512 image plane is acquired in parallel, so that dwell time is not an issue, 2) the higher quantum efficiently of a CCD detect over those typically used in confocal microscopy (although this is expected to change due to advances in confocal detector technology), and 3) because no pinhole is used to reject light, a much larger fraction of the emitted light is collected. Thus we can typically acquire images with thousands of photons per pixel using a mercury lamp, instead of a laser, for illumination. The use of low excitation light is critical for living samples, and also reduces bleaching. The high speed of widefield microscopy is also essential for time-lapse 3D microscopy, since one must acquire images quickly enough to resolve interesting events.

Gamma-ray imaging with an image intensifier and a cooled CCD camera

2001 ◽  
Author(s):  
Naoki Saitoh ◽  
Kenro Kuroki ◽  
Kenji Kurosawa ◽  
Norimitsu Akiba
Keyword(s):  
Gamma Ray ◽  
Image Intensifier ◽  
Ccd Camera ◽  
Gamma Ray Imaging ◽  
Cooled Ccd

scholarly journals Multiscale 2D-SPR Biosensing for Cell Chips

2007 ◽  
Vol 19 (5) ◽  
pp. 519-523 ◽  
Author(s):  
Masayasu Suzuki ◽  
◽  
Toyohiro Ohshima ◽  
Shintaro Hane ◽  
Yasunori Iribe ◽  
...  
Keyword(s):  
Protein A ◽  
Cell Activity ◽  
Ccd Camera ◽  
Pdms Film ◽  
Sensor Film ◽  
Spr Imaging ◽  
Measurement Protocol ◽  
A Cell ◽  
Cooled Ccd ◽  
Imaging Sensor

Evaluating cell activity and functions in different-sized cell chambers requires multiscale sensing. We have been developing multiscale biosensing applied from 10 µm to 1 mm. We measured mouse IgG in micro wells using a high-resolution two-dimensional surface plasmon resonance (SPR) imaging affinity sensor. This sensor uses high refractive optics, a 1X to 7X microscopic lens, and a cooled CCD camera. The micro-well array was prepared with a PDMS film on gold sensor film. Protein A immobilized on sensor film was used for IgG recognition. SPR sensitivity was dramatically decreased with 10 and 8.5 µm microwells. To improve sensor sensitivity, we optimized the sensor’s measurement angle and exposure time, enabling mouse IgG to be detected in wells of 1 mm, 30 µm, and 10 µm using the same 2D-SPR imaging sensor and measurement protocol. These results show the feasibility of multiscale biosensing use in antibody production in a micro well or a cell chamber.

Recent Development of Cone-Beam X-Ray Microtomography at Sunyab

1997 ◽  
Vol 3 (S2) ◽  
pp. 1125-1126
Author(s):  
S.J. Pan ◽  
A. Shih ◽  
W.S. Liou ◽  
M.S. Park ◽  
G. Wang ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Imaging System ◽  
Tomographic Reconstruction ◽  
Ccd Camera ◽  
Test Sample ◽  
Glass Beads ◽  
Cone Beam ◽  
Projection Data ◽  
X Ray ◽  
Cooled Ccd

An experimental X-ray cone-beam microtomographic imaging system utilizing a generalized Feldkamp reconstruction algorithm has been developed in our laboratory. This microtomographic imaging system consists of a conventional dental X-ray source (Aztech 65, Boulder, CO), a sample position and rotation stage, an X-ray scintillation phosphor screen, and a high resolution slow scan cooled CCD camera (Kodak KAF 1400). A generalized Feldkamp cone-beam algorithm was used to perform tomographic reconstruction from cone-beam projection data. This algorithm was developed for various hardware configuration to perform reconstruction of spherical, rod-shaped and plate-like specimen.A test sample consists of 8 glass beads (approx. 800μm in diameter) dispersed in an epoxy-filled #0 gelatin capsule. One hundred X-ray projection images were captured equal angularly (at 3.6 degree spacing) by the cooled CCD camera at a of 1317×967 (17×17mm2) pixels with 12-bit dynamic range. Figure 1 shows a 3D isosurface rendering of the test sample. The eight glass beads and trapped air bubbles (arrows) in the epoxy resin (e) are clearly visible.

Multi-Mode Light Microscopy of Microtubule and Endoplasmic Reticulum Dynamics in Migrating Newt Epithelial Cells.

1997 ◽  
Vol 3 (S2) ◽  
pp. 211-212
Author(s):  
C. M. Waterman-Storer ◽  
E. D. Salmon
Keyword(s):  
Digital Imaging ◽  
Imaging System ◽  
Ccd Camera ◽  
Diaphragm Position ◽  
Multiple Band ◽  
Fluorescence Light ◽  
Band Pass ◽  
Cooled Ccd ◽  
System 1 ◽  
Multi Mode

We have developed a multi-mode digital imaging system (1-3) which acquires images with a 12 bit cooled CCD camera. A multiple band pass dichromatic mirror and robotically controlled excitation filter wheels provide rapid wavelength selection for epi-fluorescence with DAPI, fluorescein or GFP and X-rhodamine fluorophores while maintaining image registration on the cooled CCD detector. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. A robotically controlled emission filter wheel in front of the CCD camera inserts an analyzer in the light path for DIC imaging. To maximize fluorescence light intensity, the analyzer is removed and an optical flat of equivalent optical thickness is inserted for fluorescence imaging. A slider is inserted at the field diaphragm position of the fluorescence epi-illuminator to provide in-focus slit and spot targets for 360 nm photoactivation of “caged” fluorophores. The microscope system is robotically controlled and image acquisition and analysis is performed using MetaMorph™ digital imaging software.

Characteristics relevant to portal dosimetry of a cooled CCD camera-based EPID

2004 ◽  
Vol 31 (9) ◽  
pp. 2549-2551 ◽  
Author(s):  
E. M. Franken ◽  
J. C. J. de Boer ◽  
J. C. Barnhoorn ◽  
B. J. M. Heijmen
Keyword(s):  
Ccd Camera ◽  
Portal Dosimetry ◽  
Cooled Ccd

A Peltier-cooled CCD Camera

1991 ◽  
Vol 9 (1) ◽  
pp. 158-159 ◽  
Author(s):  
B. D. Carter ◽  
M. C. B. Ashley
Keyword(s):  
Dark Current ◽  
Cooling System ◽  
Ccd Camera ◽  
Radiative Heating ◽  
Peltier Effect ◽  
Charge Coupled Device ◽  
Automated Imaging ◽  
Cooled Ccd ◽  
Ccd Detectors ◽  
Careful Design

AbstractWe describe the application of Peltier effect cooling to charge coupled device (CCD) detectors. We are developing this technique to produce a CCD camera which requires low maintenance, yet has sufficiently small dark-current for long exposure imaging. This camera will be used in an automated imaging telescope at Siding Spring Observatory. The design principles used to maximise cooling of the detector, and hence minimise dark-current, are discussed. A small dark-current can be obtained only if great care is taken to reduce or eliminate convective, conductive and radiative heating of the chip. In addition, a path of high thermal conductivity must be provided for the heat removed from the CCD. A recent laboratory test of our cooling system demonstrates that careful design can lead to sufficiently low CCD dark-current for many astronomical applications.

Comparison of Vibrio and Firefly Luciferases as Reporter Gene Systems for Use in Bacteria and Plants

1996 ◽  
Vol 23 (1) ◽  
pp. 75 ◽  
Author(s):  
SR Mudge ◽  
WR Lewis-Henderson ◽  
RG Birch
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Ccd Camera ◽  
Reporter Gene Expression ◽  
In Vitro Assays ◽  
E Coli ◽  
Cell Extracts ◽  
Cooled Ccd

Luciferase genes from Vibrio harveyi (luxAB) and firefly (luc) were introduced into E. coli, Agrobacteriurn, Arabidopsis and tobacco. Transformed bacteria and plants were quantitatively assayed for luciferase activity using a range of in vitro and in vivo assay conditions. Both lux and luc proved efficient reporter genes in bacteria, although it is important to be aware that the sensitive assays may detect expression due to readthrough from distant promoters. LUX activity was undetectable by liquid nitrogen-cooled CCD camera assays on intact tissues of plants which showed strong luxAB expression by in vitro assays. The decanal substrate for the lux assay was toxic to many plant tissues, and caused chemiluminescence in untransformed Arabidopsis leaves. These are serious limitations to application of the lux system for sensitive, non-toxic assays of reporter gene expression in plants. In contrast, LUC activity was readily detectable in intact tissues of all plants with luc expression detectable by luminometer assays on cell extracts. Image intensities of luc-expressing leaves were commonly two to four orders of magnitude above controls under the CCD camera. Provided adequate penetration of the substrate luciferin is obtained, luc is suitable for applications requiring sensitive, non-toxic assays of reporter gene expression in plants.

scholarly journals Using CCD’s in Introductory-Level College Astronomy Laboratories

1990 ◽  
Vol 105 ◽  
pp. 142-144
Author(s):  
Thomas J. Balonek
Keyword(s):  
Dark Current ◽  
Software Package ◽  
Multiple Access ◽  
Ccd Camera ◽  
Analysis Software ◽  
Camera System ◽  
Black And White ◽  
Standard Data ◽  
College Astronomy ◽  
Cooled Ccd

Our university recently purchased a liquid-nitrogen-cooled CCD camera system (from Photometrics Ltd., Tucson, Arizona) which has been installed on our campus’ 40-cm multiple access (Cassegrain/Newtonian) telescope. Images are reduced online at the observatory using Photometrics’ microcomputer-based analysis software package, which includes operations for standard data acquisition and initial stages of data reduction — including corrections for bias, dark current, and flat fielding. Images are displayed on a 256-level-gray-scale black and white monitor. Additional post-processing can be done either on the CCD system’s computer at the observatory or on IBM-AT/PC’s located both at the observatory and in the laboratory.

Optical Odor Imaging by Fluorescence Probes

2012 ◽  
Vol 24 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Hirotaka Matsuo ◽  
◽  
Yudai Furusawa ◽  
Masashi Imanishi ◽  
Seiichi Uchida ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Ccd Camera ◽  
Research Area ◽  
Fluorescence Probes ◽  
Response Patterns ◽  
Odor Compounds ◽  
Active Research ◽  
Cooled Ccd ◽  
Detection Of Explosives ◽  
Fluorescence Dyes

Odor gas detection is important for the detection of explosives, environmental sensing, biometrics, foodstuffs and a comfortable life. Such odor-source localizations is an active research area for robotics. In this study, we tried to detect odor chemicals with an optical method that can be applied for the spatiotemporal detection of odor. We used four types of fluorescence dyes; tryptophan, quinine sulfate, acridine orange, and 1-anilinonaphthalene-8-sulfonate (ANS). As analyses, we measured the following four odor chemicals, 2-furaldehyde, vanillin, acetophenone, and benzaldehyde. The fluorescence-quenching mechanism of PET (Photoinduced Electron Transfer) or FRET (Fluorescence Resonance Electron Transfer), which occur between fluorescence dyes and odor compounds, could prevent unintended detection of various odorants that is caused by their unspecific adsorption onto the detecting materials. The fluorescence changes were then observed. Thus, we could detect the odor substances through fluorescent quenching by using the fluorescence dyes. Odor information could be obtained by response patterns across all the fluorescence dyes. Moreover, we captured odor images with a cooled CCD camera. Shapes of the targets that emitted odor could be roughly recognized by the odor-shape images. From the spatiotemporal images of odors, twodimensional odor expanse could be obtained.

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