Depth penetration limit to optical coherence microscopy in turbid media: the effect of multiply scattered light detection on image contrast and resolution

2001 ◽  
Author(s):  
Kostadinka K. Bizheva ◽  
David A. Boas
Keyword(s):  
Scattered Light ◽  
Image Contrast ◽  
Turbid Media ◽  
Optical Coherence ◽  
Light Detection ◽  
Optical Coherence Microscopy

scholarly journals Enhancement of optical coherence microscopy in turbid media by an optical parametric amplifier

2014 ◽  
Vol 8 (6) ◽  
pp. 512-521 ◽  
Author(s):  
Youbo Zhao ◽  
Haohua Tu ◽  
Yuan Liu ◽  
Andrew J. Bower ◽  
Stephen A. Boppart
Keyword(s):  
Parametric Amplifier ◽  
Turbid Media ◽  
Optical Parametric Amplifier ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Optical Parametric

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

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