Does digital deconvolution improve two-photon microscopy in deep tissue imaging?

2001 ◽  
Author(s):  
Colten R. Noakes ◽  
Toshiyasu Goto ◽  
Raymond Keller ◽  
Ammasi Periasamy
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

2017 ◽  
Vol 8 (11) ◽  
pp. 7696-7704 ◽  
Author(s):  
Yong Woong Jun ◽  
Hye Rim Kim ◽  
Ye Jin Reo ◽  
Mingchong Dai ◽  
Kyo Han Ahn
Keyword(s):  
Wavelength Region ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Deep Tissue Imaging

We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.

scholarly journals Advantages of Non-degenerate Two-photon Microscopy for Deep Tissue Imaging

2020 ◽  
Vol 118 (3) ◽  
pp. 311a
Author(s):  
Sanaz Sadegh ◽  
Mu-Han Yang ◽  
Christopher Ferri ◽  
Martin Thunemann ◽  
Anna Devor ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

2015 ◽  
Vol 6 (3) ◽  
pp. 1944-1948 ◽  
Author(s):  
Pablo Rivera-Fuentes ◽  
Alexandra T. Wrobel ◽  
Melissa L. Zastrow ◽  
Mustafa Khan ◽  
John Georgiou ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Live Cell ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
Acidic Vesicles ◽  
Unambiguous Detection

A fluorescent sensor that is pH-insensitive and compatible with two-photon microscopy was developed and applied to live cell and deep tissue imaging.

Two-Photon Microscopy for Deep Tissue Imaging of Living Specimens

2012 ◽  
Vol 20 (4) ◽  
pp. 12-16
Author(s):  
Tilman Franke ◽  
Sebastian Rhode
Keyword(s):  
Three Dimensional ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Time Resolved ◽  
3D Images ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
And Function ◽  
Structural Contrast

Two-photon microscopy (2PM) provides three-dimensional (3D) and four-dimensional (4D) (x, y, z, t) imaging in living specimens or under experimental physiological conditions very close to live. In conjunction with fluorescent labels, 2PM provides a powerful means of investigating the relationships between structure and function at the microscopic level that are key to understanding biological systems. This technique is able to provide time-resolved, 3D images of dynamic systems with near-diffraction-limited resolution and highly specific structural contrast.

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

scholarly journals Extremely High Brightness from Polymer-Encapsulated Quantum Dots for Two-photon Cellular and Deep-tissue Imaging

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yanyan Fan ◽  
Helin Liu ◽  
Rongcheng Han ◽  
Lu Huang ◽  
Hao Shi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
High Brightness ◽  
Two Photon ◽  
Deep Tissue Imaging

Lysosome-targetable polythiophene nanoparticles for two-photon excitation photodynamic therapy and deep tissue imaging

2017 ◽  
Vol 5 (20) ◽  
pp. 3651-3657 ◽  
Author(s):  
Shaojing Zhao ◽  
Guangle Niu ◽  
Feng Wu ◽  
Li Yan ◽  
Hongyan Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Quantum Yield ◽  
Fluorescence Imaging ◽  
Cross Section ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Photon Excitation ◽  
Deep Tissue Imaging ◽  
Two Photon Excitation

Polythiophene nanoparticles with large TPA cross section and high1O2generation quantum yield have been developed for simultaneous lysosome-targetable fluorescence imaging and photodynamic therapy.

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