Bleed-through and photobleaching correction in multiphoton FRET microscopy

Author(s):  
Masilamani Elangovan ◽  
Ammasi Periasamy
Keyword(s):  
2019 ◽  
Vol 58 (2) ◽  
pp. 170-182
Author(s):  
Karen F. Quandt

Baudelaire refers in his first essay on Théophile Gautier (1859) to the ‘fraîcheurs enchanteresses’ and ‘profondeurs fuyantes’ yielded by the medium of watercolour, which invites a reading of his unearthing of a romantic Gautier as a prescription for the ‘watercolouring’ of his own lyric. If Paris's environment was tinted black as a spiking population and industrial zeal made their marks on the metropolis, Baudelaire's washing over of the urban landscape allowed vivid colours to bleed through the ‘fange’. In his early urban poems from Albertus (1832), Gautier's overall tint of an ethereal atmosphere as well as absorption of chaos and din into a lulling, muted harmony establish the balmy ‘mise en scène’ that Baudelaire produces at the outset of the ‘Tableaux parisiens’ (Les Fleurs du mal, 1861). With a reading of Baudelaire's ‘Tableaux parisiens’ as at once a response and departure from Gautier, or a meeting point where nostalgia ironically informs an avant-garde poetics, I show in this paper how Baudelaire's luminescent and fluid traces of color in his urban poems, no matter how washed or pale, vividly resist the inky plumes of the Second Empire.


2001 ◽  
Vol os-10 (1) ◽  
pp. 1558925001os-10
Author(s):  
William H. Pound

Studies were carried out in a nonwoven roll goods plant to help eliminate subjective formation ratings. Tests were run on single layer samples with instruments measuring color, transmittance, haze and camera image gray scale values. High correlations are shown with air porosity, basis weight and formation ratings. Relationships to diaper glue bleed through, superabsorbent loss, and web tracking are reported. Some effects of process change are noted. Correlations critically depend upon measuring identical sample areas.


2018 ◽  
Vol 4 (5) ◽  
pp. 68 ◽  
Author(s):  
Muhammad Hanif ◽  
Anna Tonazzini ◽  
Pasquale Savino ◽  
Emanuele Salerno

2008 ◽  
Vol 72 (1-3) ◽  
pp. 57-70 ◽  
Author(s):  
Mariana S.C. Almeida ◽  
Luís B. Almeida
Keyword(s):  

2018 ◽  
Author(s):  
Jongjin Lee ◽  
Sangjun Park ◽  
Sungchul Hohng

Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.


1998 ◽  
Vol 38 (8-9) ◽  
pp. 409-416 ◽  
Author(s):  
Steve Krugel ◽  
Leslie Nemeth ◽  
Craig Peddie

The Greater Vancouver Regional District (GVRD) is a major regional agency managing wastewater collection and treatment for Vancouver, Canada and surrounding municipalities. As part of their $ 600 million program to upgrade 2 major plants to secondary treatment, the GVRD was faced with the requirement to produce a treated biosolids meeting the equivalent of a U.S. EPA Class A product to assure success of their new biosolids beneficial use program. Various alternatives to achieve a Class A product were evaluated in detail. The GVRD selected a new and innovative process which they termed extended thermophilic anaerobic digestion. The characteristics of this process which help achieve required pathogen kills are the thermophilic operating temperatures and the series operation of reactors, cutting the bleed-through of pathogens observed in conventional complete mix systems. The new thermophilic digesters have been in operation since 1996 and the flow-through vessels required to complete “extended” operation will be complete in late 1998.


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