Chronic imaging of amyloid plaques in the live mouse brain using multiphoton microscopy

2001 ◽  
Author(s):  
Brian J. Bacskai ◽  
Stephen T. Kajdasz ◽  
R. H. Christie ◽  
Warren R. Zipfel ◽  
Rebecca M. Williams ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Multiphoton Microscopy ◽  
Amyloid Plaques ◽  
Live Mouse

scholarly journals Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

10.3791/3848 ◽  
2012 ◽  
Author(s):  
Sonia G. Parra ◽  
Sam S. Vesuna ◽  
Teresa A. Murray ◽  
Michael J. Levene
Keyword(s):  
Mouse Brain ◽  
Multiphoton Microscopy

scholarly journals Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Nelda Antonovaite ◽  
Steven V. Beekmans ◽  
Elly M. Hol ◽  
Wytse J. Wadman ◽  
Davide Iannuzzi
Keyword(s):  
Brain Tissue ◽  
Mouse Brain ◽  
Regional Variations ◽  
Live Mouse

High throughput object-based image analysis of β-amyloid plaques in human and transgenic mouse brain

2012 ◽  
Vol 204 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Harry D. Samaroo ◽  
Alan C. Opsahl ◽  
Jan Schreiber ◽  
Sharon M. O’Neill ◽  
Michael Marconi ◽  
...  
Keyword(s):  
Image Analysis ◽  
Transgenic Mouse ◽  
Mouse Brain ◽  
High Throughput ◽  
Amyloid Plaques ◽  
Object Based Image Analysis ◽  
Object Based ◽  
Β Amyloid

scholarly journals Citrate‐assisted efficient local delivery of naked oligonucleotide into live mouse brain cells

2019 ◽  
Vol 52 (4) ◽  
Author(s):  
Haibin Zhou ◽  
Shouhua Zhang ◽  
Fei Lv ◽  
Wenzhi Sun ◽  
Lihua Wang ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Local Delivery ◽  
Brain Cells ◽  
Live Mouse

A SERS Optophysiological Probe for the Real‐Time Mapping and Simultaneous Determination of the Carbonate Concentration and pH Value in a Live Mouse Brain

2019 ◽  
Vol 131 (16) ◽  
pp. 5310-5314 ◽  
Author(s):  
Weikang Wang ◽  
Fan Zhao ◽  
Mingzhi Li ◽  
Chuanping Zhang ◽  
Yuanhua Shao ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Determination ◽  
Mouse Brain ◽  
Ph Value ◽  
The Real ◽  
Carbonate Concentration ◽  
Live Mouse

scholarly journals Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain

NeuroImage ◽  
2011 ◽  
Vol 54 (1) ◽  
pp. 113-122 ◽  
Author(s):  
T.M. Wengenack ◽  
D.A. Reyes ◽  
G.L. Curran ◽  
B.J. Borowski ◽  
J. Lin ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Regional Differences ◽  
Amyloid Plaques

scholarly journals Deep Learning-powered Bessel-beam Multi-parametric Photoacoustic Microscopy

2021 ◽  
Author(s):  
Yifeng Zhou ◽  
Naidi Sun ◽  
Song Hu
Keyword(s):  
Deep Learning ◽  
High Resolution ◽  
Gaussian Beam ◽  
Mouse Brain ◽  
Bessel Beam ◽  
Large Field ◽  
Depth Range ◽  
Photoacoustic Microscopy ◽  
Beam Excitation ◽  
Live Mouse

Enabling simultaneous and high resolution quantification of the total concentration of hemoglobin (CHb), oxygen saturation of hemoglobin (sO2), and cerebral blood flow (CBF), multi parametric photoacoustic microscopy (PAM) has emerged as a promising tool for functional and metabolic imaging of the live mouse brain. However, due to the limited depth of focus imposed by the Gaussian beam excitation, the quantitative measurements become inaccurate when the imaging object is out of focus. To address this problem, we have developed a hardware-software combined approach by integrating Bessel beam excitation and conditional generative adversarial network (cGAN) based deep learning. Side by side comparison of the new cGAN powered Bessel-beam multi parametric PAM against the conventional Gaussian beam multi parametric PAM shows that the new system enables high resolution, quantitative imaging of CHb, sO2, and CBF over a depth range of ~600 μm in the live mouse brain, with errors 13 to 58 times lower than those of the conventional system. Better fulfilling the rigid requirement of light focusing for accurate hemodynamic measurements, the deep learning powered Bessel beam multi parametric PAM may find applications in large field functional recording across the uneven brain surface and beyond (e.g., tumor imaging).

scholarly journals Novel botanical therapeutic NB-02 effectively treats Alzheimer’s neuropathophysiology in an  APP/PS1 mouse model

2020 ◽  
Author(s):  
Yee Fun Lee ◽  
Amanda Lariviere ◽  
Alyssa N Russ ◽  
Sang-Zin Choi ◽  
Brian Bacskai ◽  
...  
Keyword(s):  
Animal Model ◽  
Neurodegenerative Disorder ◽  
Multiphoton Microscopy ◽  
Amyloid Plaques ◽  
Amyloid Plaque ◽  
Brain Parenchyma ◽  
Neuronal Function ◽  
Calcium Dysregulation ◽  
Plaque Deposition

Abstract Background Alzheimer’s disease (AD) is an incurable neurodegenerative disorder and a major cause of dementia. Some of the hallmarks of AD include presence of amyloid plaques in brain parenchyma, calcium dysregulation within individual neurons, and neuroinflammation. A promising therapeutic would reverse or stymie these pathophysiologies in an animal model of AD. Methods We tested the effect of NB-02, previously known as DA-9803, a novel multimodal therap­eutic, on amyloid deposition, neuronal calcium regulation and neuroinflammation in 8-10 month old APP mice, an animal model of AD.Results In vivo multiphoton microscopy revealed that 2 month-long administration of NB-02 halted amyloid plaque deposition and cleared amyloid in the cortex. Post-mortem analysis verified NB-02-dependent decrease in plaque deposition in the cortex as well as hippocampus. Furthermore, drug treatment reversed neuronal calcium elevations, thus restoring neuronal function. Finally, NB-02 transformed the morphology of astrocytes and microglia to a more phagocytic state, affecting neuroinflammation. Conclusions NB-02 was effective at reversing AD neuropathophysiology in an animal model. Therefore, in addition to serving as a promising preventative agent, NB-02 holds potential as a treatment for AD in the clinic.

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