A SERS Optophysiological Probe for the Real‐Time Mapping and Simultaneous Determination of the Carbonate Concentration and pH Value in a Live Mouse Brain

2019 ◽  
Vol 131 (16) ◽  
pp. 5310-5314 ◽  
Author(s):  
Weikang Wang ◽  
Fan Zhao ◽  
Mingzhi Li ◽  
Chuanping Zhang ◽  
Yuanhua Shao ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Determination ◽  
Mouse Brain ◽  
Ph Value ◽  
The Real ◽  
Carbonate Concentration ◽  
Live Mouse

DESIGN AND EVALUATION OF A NEW ELECTROPHORETIC MOBILITY MEASUREMENT INSTRUMENT USING MICROELECTROPHORESIS SYSTEM WITH IMAGE PROCESSING METHOD

2017 ◽  
Vol 29 (05) ◽  
pp. 1750036
Author(s):  
Boon Yew Teoh ◽  
Misni Misran ◽  
Zhi Zhang Tan ◽  
Poh Foong Lee
Keyword(s):  
Image Processing ◽  
Real Time ◽  
Electrophoretic Mobility ◽  
Bone Cells ◽  
Ph Value ◽  
Processing Method ◽  
Yeast Cells ◽  
Polystyrene Microsphere ◽  
The Real ◽  
Image Processing Method

Electrophoretic mobility (EPM) measurement on biological particles in fluids is well established. The current method in measuring EPM is using laser which the target particles are not visible. Additional morphology information is critical for the EPM measurement. Image processing is a promising method to obtain the EPM together with the morphology information. In this study, a setup of micro electrophoresis system with a compact CCD microscope was constructed. This setup was equipped with image processing method for capturing the images of the moving particles in an electric field. With the image processing method (Horn–Schunck method), the images captured were processed in real time to obtain the EPM of the particle. Velocity of the particles was then measured and the particles’ EPM was obtained. With the captured images of the particles in real time, the system can present the image of the targeted particle together with the EPM value. The setup of this prototype was calibrated with discrete particles (Polystyrene microsphere size of 10[Formula: see text][Formula: see text]m[Formula: see text] 5%) and with a magnification value of 125[Formula: see text]X. This system is suitable for the surface charge measurement of discrete particle with size in between 4[Formula: see text][Formula: see text]m and 20[Formula: see text][Formula: see text]m. Comparison of commercialized device with our laboratory setup for calibration on EPM of polystyrene beads had a variance of solely 13%. Measurement on yeast cells, normal (hFob 1.19) and cancer bone cells (U2OS) indicated that the EPM of yeast became highly negative in the pH value of 4.5 and 6.5. The negative EPM of the cancer cell is slightly larger than that of the normal cell for pH ranging from 4.4 to 5.0. In conclusion, the real-time EPM measurement set up for this study is able to display the real-time images of the moving particles in fluid suspension during measurement.

scholarly journals Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Kaltenbrunner ◽  
Walter Mayer ◽  
Kirsten Kerkhoff ◽  
Rita Epp ◽  
Hermann Rüggeberg ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Processed Food ◽  
Domestic Pig ◽  
The Real ◽  
Qualitative And Quantitative ◽  
Pcr Assays

Abstract Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

scholarly journals Determination of ABO blood group genotypes using the real-time loop-mediated isothermal amplification method

2015 ◽  
Vol 12 (4) ◽  
pp. 5963-5966 ◽  
Author(s):  
CHAO ZHANG ◽  
JUANLI ZHU ◽  
JIANGCUN YANG ◽  
YINSHENG WAN ◽  
TING MA ◽  
...  
Keyword(s):  
Real Time ◽  
Blood Group ◽  
Isothermal Amplification ◽  
Abo Blood Group ◽  
The Real ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Time Loop

Development of a highly sensitive real-time immuno-PCR for the measurement of chloramphenicol in milk based on magnetic bead capturing

2014 ◽  
Vol 6 (23) ◽  
pp. 9340-9347 ◽  
Author(s):  
Xiaoqi Tao ◽  
Zhifei He ◽  
Xingyuan Cao ◽  
Jianzhong Shen ◽  
Hongjun Li
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Magnetic Bead ◽  
The Real ◽  
Highly Sensitive

The real-time immuno-quantitative PCR (RT-IPCR) schematic illustration of the determination of CAP in milk based on magnetic bead capturing.

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