Confocal spectral imaging by microspectrofluorometry using two-photon excitation: application to the study of anticancer drugs within single living cancer cells

1999 ◽  
Author(s):  
Igor Chourpa ◽  
Manuela Pereira ◽  
Jean-Marc Millot ◽  
Hamid Morjani ◽  
Michel Manfait
Keyword(s):  
Cancer Cells ◽  
Anticancer Drugs ◽  
Spectral Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Confocal Spectral Imaging ◽  
Single Living ◽  
Two Photon Excitation

Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

2008 ◽  
Vol 19 (31) ◽  
pp. 315102 ◽  
Author(s):  
Lissett Bickford ◽  
Jiantang Sun ◽  
Kun Fu ◽  
Nastassja Lewinski ◽  
Vengadesan Nammalvar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Spectral Imaging ◽  
Gold Nanoshells ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Fluorescence detection of camptothecin anticancer drugs by two-photon excitation

1998 ◽  
Author(s):  
Thomas G. Burke ◽  
Magda Malak ◽  
David Bom ◽  
Dennis P. Curran ◽  
Henryk M. Malak ◽  
...  
Keyword(s):  
Anticancer Drugs ◽  
Fluorescence Detection ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Photo-induced mitochondrial DNA damage and NADH depletion by –NO2 modified Ru(ii) complexes

2021 ◽  
Vol 57 (34) ◽  
pp. 4162-4165
Author(s):  
Shuang Qi ◽  
Zhihui Jin ◽  
Yao Jian ◽  
Yuanjun Hou ◽  
Chao Li ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Cancer Cells ◽  
Good Activity ◽  
Two Photon ◽  
Mitochondrial Dna Damage ◽  
Photon Excitation ◽  
First Time ◽  
Two Photon Excitation

Ru(ii) PACT agents with a synchronous photo-catalyzed NADH depletion ability were reported for the first time and displayed good activity towards cisplatin-resistant cancer cells upon one- and two-photon excitation.

Determination of two-photon excitation and emission spectra of fluorescent molecules in single living cells

2008 ◽  
Author(s):  
Valerică Raicu ◽  
Anurag Chaturvedi ◽  
Michael Stoneman ◽  
Giorgi Petrov ◽  
Russell Fung ◽  
...  
Keyword(s):  
Emission Spectra ◽  
Living Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Single Living Cells ◽  
Fluorescent Molecules ◽  
Single Living ◽  
Two Photon Excitation

scholarly journals Tumour-targeting photosensitisers for one- and two-photon activated photodynamic therapy

2019 ◽  
Vol 17 (27) ◽  
pp. 6585-6594 ◽  
Author(s):  
Sébastien Jenni ◽  
Angélique Sour ◽  
Frédéric Bolze ◽  
Barbara Ventura ◽  
Valérie Heitz
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Near Infrared ◽  
Tumour Targeting ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Efficient receptor-mediated delivery of a folate-targeted photosensitiser to kill cancer cells following two-photon excitation in the near-infrared is demonstrated.

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two-photon excitation fluorescence microscopy in living systems

1993 ◽  
Vol 51 ◽  
pp. 154-155 ◽  
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Singlet State ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two‐photon excitation fluorescence microscopy using a semiconductor laser

Bioimaging ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 70-75 ◽  
Author(s):  
Pekka E Hänninen ◽  
Martin Schrader ◽  
Erkki Soini ◽  
Stefan W Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Semiconductor Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-Photon Excitation Imaging of Glucose Metabolism in Living Tissue

1997 ◽  
Vol 3 (S2) ◽  
pp. 305-306
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Collection Efficiency ◽  
Three Dimensional ◽  
Spatial Filter ◽  
Input Power ◽  
Photon Absorption ◽  
Focal Volume ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. It provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. This results in inherent submicron optical sectioning by excitation alone. In practice, TPEM is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 limits the average input power to less than 10 mW, only slightly greater than the power normally used in confocal microscopy. Because of the intensity-squared dependence of the two-photon absorption, the excitation is limited to the focal volume.

Sign in / Sign up

Export Citation Format

Share Document