Can tumor cell suspension serve as an optical model of tumor tissue in situ?

1999 ◽  
Author(s):  
Anna N. Yaroslavsky ◽  
Anja Vervoorts ◽  
Alexander V. Priezzhev ◽  
Ilya V. Yaroslavsky ◽  
Joerg G. Moser ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Suspension ◽  
Optical Model ◽  
Tumor Tissue ◽  
Tumor Cell Suspension

Hematogeneous Pulmonary Metastasis

1993 ◽  
Vol 34 (6) ◽  
pp. 581-585 ◽  
Author(s):  
W. S. Kim ◽  
J.-G. Im ◽  
E. C. Chung ◽  
M. H. Han ◽  
J. K. Han ◽  
...  
Keyword(s):  
High Resolution ◽  
Tumor Cell ◽  
Cell Suspension ◽  
Experimental Model ◽  
Pulmonary Metastasis ◽  
Visual Inspection ◽  
Pulmonary Metastases ◽  
Chest Radiographs ◽  
Tumor Cell Suspension ◽  
Pathologic Findings

The purpose of this study was to develop an experimental model of pulmonary metastases using VX-2 tumor, and to describe sequential radiologic and pathologic findings with special attention to the interstitial changes around the metastatic nodules. Through ear veins of 25 rabbits, VX-2 tumor cell suspension was injected with 0.8-mm scalp needles. Chest radiographs were taken every other day after tumor injection. The rabbits were sacrificed at scheduled times from 3 to 30 days after tumor injection. The inflated and fixed lungs were examined with visual inspection, low-kV radiography, high resolution CT (HRCT), microradiography of the sliced lung and with histopathologic studies. Hematogeneous pulmonary metastases occurred in 24 of 25 rabbits. In addition to the metastatic nodules, perinodular and peribronchovascular infiltrations were seen on low-kV radiography, HRCT, microradiography and histologic studies.

scholarly journals THE EFFECT OF TESTICLE EXTRACT AND OF NORMAL SERUM ON A TRANSPLANTABLE EPITHELIAL TUMOR OF THE RABBIT

1931 ◽  
Vol 54 (4) ◽  
pp. 493-498 ◽  
Author(s):  
F. Duran-Reynals
Keyword(s):  
Tumor Cell ◽  
Cell Suspension ◽  
Pathogenic Bacteria ◽  
Normal Serum ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Epithelial Tumor ◽  
Tumor Cell Suspension

Extract of rat, rabbit or bull testicle prevents or retards the growth of a rabbit tumor when a mixture of the extract and a tumor cell suspension is inoculated intradermally. Similar mixtures, made with normal rabbit serum instead of testicle extract, give rise to tumors which grow with unusual rapidity. The results are the opposite of those obtained with pathogenic bacteria or filtrable viruses which are enhanced by testicle extract and generally inhibited by normal serum.

scholarly journals TMOD-24. GENERATION AND CHARACTERIZATION OF A NOVEL TRANSGENIC IDO REPORTER MOUSE FOR IDO POSTTRANSLATIONAL MODIFICATION ANALYSIS IN SITU

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii233-ii233
Author(s):  
April Bell ◽  
Lijie Zhai ◽  
Erik Ladomersky ◽  
Kristen Lauing ◽  
Lakshmi Bollu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tumor Cell ◽  
Central Nervous System Tumor ◽  
Post Translational Modifications ◽  
Reporter Mouse ◽  
C Terminus ◽  
Human Gbm

Abstract Glioblastoma (GBM) is the most common and aggressive primary central nervous system tumor in adults with a median survival of 14.6 months. GBM is a potently immunosuppressive cancer due in-part to the prolific expression of immunosuppressive indoleamine 2,3 dioxygenase 1 (IDO). Tumor cell IDO facilitates the intratumoral accumulation of regulatory T cells (Tregs; CD4+CD25+FoxP3+). Although immunosuppressive IDO activity is canonically characterized by the conversion of tryptophan into kynurenine, we have utilized transgenic and syngeneic mouse models and mutant glioma lines to demonstrate that tumor cell IDO increases Treg accumulation independent of tryptophan metabolism. Here, we address the gap in our understanding of IDO signaling activity in vivo. Subcutaneously-engrafted human GBM expressing human IDO-GFP cDNA was isolated from immunodeficient humanized NSG-SGM3 mice. The tumor was immunoprecipitated for the GFP tag using GFP-TRAP followed by mass spectrometry which revealed a novel methylation site on a lysine residue at amino acid 373 in the IDO C-terminus region. Western blot analysis of IDO protein also revealed the presence of tyrosine phosphorylation. Additionally, we recently created a new transgenic IDO reporter mouse model whereby endogenous IDO is fused to GFP via a T2A linker (IDO→GFP). This model allows for the isolation of IDO+ cells in real-time and without causing cell death, thereby creating the opportunity for downstream molecular analysis of in situ-isolated GFP+ cells. Collectively, our work suggests that IDO non-enzyme activity may involve the post-translational modifications we recently identified. As IDO activity may differ between in vitro and in vivo modeling systems, we will use the new IDO→GFP reporter mouse model for an improved mechanistic understanding of how immunosuppressive IDO facilitates Treg accumulation in vivo.

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