Tumor cell suspension culture on non adhesive substratum

1969 ◽  
Vol 72 (1) ◽  
pp. 24-31 ◽  
Author(s):  
O. Costăchel ◽  
L. Fadei ◽  
E. Badea
Keyword(s):  
Tumor Cell ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Tumor Cell Suspension

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

scholarly journals Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

2014 ◽  
Vol 38 ◽  
pp. 76-82 ◽  
Author(s):  
Zohreh JALALPOUR ◽  
Leila SHABANI ◽  
Ladan AFGHANI ◽  
Majid SHARIFI-TEHRANI ◽  
Sayed-Asadollah AMINI
Keyword(s):  
Suspension Culture ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Phenolic Metabolism

The reduction of starch accumulation in transgenic sugarcane cell suspension culture lines

2008 ◽  
Vol 3 (11) ◽  
pp. 1398-1406 ◽  
Author(s):  
Stephanus J. Ferreira ◽  
Jens Kossmann ◽  
James R. Lloyd ◽  
Jan-Hendrik Groenewald
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Starch Accumulation ◽  
Transgenic Sugarcane
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