Fluorescence spectroscopy of bacteriorhodopsin at room temperature

1998 ◽  
Author(s):  
Atanaska Andreeva ◽  
Villen Kolev ◽  
Tzvetana Lazarova
Keyword(s):  
Fluorescence Spectroscopy ◽  
Room Temperature

scholarly journals Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

2016 ◽  
Vol 129 ◽  
pp. 83-89 ◽  
Author(s):  
Bassam Alfarhani ◽  
Maha Al-tameemi ◽  
Agustina V. Schenone ◽  
Hector C. Goicoechea ◽  
Fernando Barbosa ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Room Temperature ◽  
Pyrene Metabolites

scholarly journals Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C′ fragment complementation system

1999 ◽  
Vol 339 (3) ◽  
pp. 721-727 ◽  
Author(s):  
Alokesh K. GHOSHAL ◽  
Chittoor P. SWAMINATHAN ◽  
Celestine J. THOMAS ◽  
Avadhesha SUROLIA ◽  
Raghavan VARADARAJAN
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Spectroscopy ◽  
Room Temperature ◽  
Intact Protein ◽  
Titration Calorimetry ◽  
Peptide Fragments ◽  
State Process ◽  
Α Helix ◽  
Kinetics Of ◽  
Covalent Complex

Escherichia coli thioredoxin was cleaved with CNBr at its single Met residue at position 37, which lies in the middle of a long α-helix. The two fragments, 1-37 and 38-108, were purified and characterized by using CD and fluorescence spectroscopy. Both fragments lack structure at neutral pH and room temperature. The secondary and tertiary structural contents of the non-covalent complex formed on the mixing of the two peptide fragments are 47% and 35% of the intact protein respectively. The thermodynamics and kinetics of fragment association were characterized by titration calorimetry and stopped-flow fluorescence spectroscopy. Single phases were observed for both association and dissociation, with rate constants at 298 K of kon = 4971±160 M-1·s -1 and koff = 0.063±0.009 s-1 respectively. The ratio kon/koff was very similar to the binding constant determined by titration calorimetry, suggesting that binding is a two-state process. The values for ∆Cp, ∆H0 and ∆G0 at 298 K for dissociation of the complex were 5.7 kJ·mol-1·K-1, 45.3 kJ·mol-1 and 29.8 kJ·mol-1 respectively. The value for ∆H0 was linearly dependent on temperature from 8-40 °C, suggesting that ∆Cp is independent of temperature. The values for ∆Cp and ∆G0 are very similar to the corresponding values for the unfolding of intact thioredoxin at 25 °C. However, both ∆H0 and ∆S are significantly more positive for dissociation of the complex, suggesting a decreased hydrophobic stabilization of the complex relative to the situation for intact thioredoxin.

Fluorescence spectroscopy ofC60at room temperature

1994 ◽  
Vol 50 (1) ◽  
pp. 317-321 ◽  
Author(s):  
A. Andreoni ◽  
M. Bondani ◽  
G. Consolati
Keyword(s):  
Fluorescence Spectroscopy ◽  
Room Temperature

Polyaniline Nanostructures Doped with Fluorescent TPA-BTD-BN

2013 ◽  
Vol 807-809 ◽  
pp. 2679-2683
Author(s):  
Hang Jun Ding ◽  
Zhou Yang ◽  
He Yang ◽  
Yun Ze Long ◽  
Qing Guo He
Keyword(s):  
Electrical Properties ◽  
Fluorescence Spectroscopy ◽  
Room Temperature ◽  
Three Dimensional ◽  
Variable Range Hopping ◽  
Strong Fluorescence ◽  
Exponential Temperature Dependence ◽  
Composite Pellets ◽  
Template Free ◽  
Dimensional Variable

In this paper, a novel fluorescent material with high conductivity as 0.45 S·cm-1 and strong fluorescence has been successfully synthesized basing on polyaniline (PANI) nanostructures doped with a new prepared fluorescein TPABTDBN. The PANI nanostructures were prepared via a simplified template-free method (STFM) with FeCl3 as oxidant and dopant. The resulting sample was characterized by SEM, IR and fluorescence spectroscopy. The fluorescence intensity of synthesized composite improves with the increasing content of TPABTDBN. The electrical properties of pressed composite pellets were measured from room temperature about 291K down to 132K, the conductance follow the exponential temperature dependence of three-dimensional variable-range hopping (VRH) model.

Sign in / Sign up

Export Citation Format

Share Document