scholarly journals Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

2016 ◽  
Vol 129 ◽  
pp. 83-89 ◽  
Author(s):  
Bassam Alfarhani ◽  
Maha Al-tameemi ◽  
Agustina V. Schenone ◽  
Hector C. Goicoechea ◽  
Fernando Barbosa ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Room Temperature ◽  
Pyrene Metabolites

Fluorescence spectroscopy of bacteriorhodopsin at room temperature

1998 ◽  
Author(s):  
Atanaska Andreeva ◽  
Villen Kolev ◽  
Tzvetana Lazarova
Keyword(s):  
Fluorescence Spectroscopy ◽  
Room Temperature

Screening Benzo(a)pyrene Metabolites in Urine Using Synchronous Room Temperature Phosphorescence

1992 ◽  
Vol 3 (1) ◽  
pp. 17-27 ◽  
Author(s):  
M. Uziel ◽  
G. H. Miller ◽  
Randy Ward ◽  
W. Watts ◽  
T. Vo-dinh
Keyword(s):  
Room Temperature ◽  
Room Temperature Phosphorescence ◽  
Pyrene Metabolites

scholarly journals Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C′ fragment complementation system

1999 ◽  
Vol 339 (3) ◽  
pp. 721-727 ◽  
Author(s):  
Alokesh K. GHOSHAL ◽  
Chittoor P. SWAMINATHAN ◽  
Celestine J. THOMAS ◽  
Avadhesha SUROLIA ◽  
Raghavan VARADARAJAN
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Spectroscopy ◽  
Room Temperature ◽  
Intact Protein ◽  
Titration Calorimetry ◽  
Peptide Fragments ◽  
State Process ◽  
Α Helix ◽  
Kinetics Of ◽  
Covalent Complex

Escherichia coli thioredoxin was cleaved with CNBr at its single Met residue at position 37, which lies in the middle of a long α-helix. The two fragments, 1-37 and 38-108, were purified and characterized by using CD and fluorescence spectroscopy. Both fragments lack structure at neutral pH and room temperature. The secondary and tertiary structural contents of the non-covalent complex formed on the mixing of the two peptide fragments are 47% and 35% of the intact protein respectively. The thermodynamics and kinetics of fragment association were characterized by titration calorimetry and stopped-flow fluorescence spectroscopy. Single phases were observed for both association and dissociation, with rate constants at 298 K of kon = 4971±160 M-1·s -1 and koff = 0.063±0.009 s-1 respectively. The ratio kon/koff was very similar to the binding constant determined by titration calorimetry, suggesting that binding is a two-state process. The values for ∆Cp, ∆H0 and ∆G0 at 298 K for dissociation of the complex were 5.7 kJ·mol-1·K-1, 45.3 kJ·mol-1 and 29.8 kJ·mol-1 respectively. The value for ∆H0 was linearly dependent on temperature from 8-40 °C, suggesting that ∆Cp is independent of temperature. The values for ∆Cp and ∆G0 are very similar to the corresponding values for the unfolding of intact thioredoxin at 25 °C. However, both ∆H0 and ∆S are significantly more positive for dissociation of the complex, suggesting a decreased hydrophobic stabilization of the complex relative to the situation for intact thioredoxin.

Fluorescence spectroscopy ofC60at room temperature

1994 ◽  
Vol 50 (1) ◽  
pp. 317-321 ◽  
Author(s):  
A. Andreoni ◽  
M. Bondani ◽  
G. Consolati
Keyword(s):  
Fluorescence Spectroscopy ◽  
Room Temperature

scholarly journals BILIARY PAH METABOLITES IN FISH FROM THE HIGHLY IMPACTED GUANABARA BAY, IN SOUTHEASTERN BRAZIL, DETERMINED BY FIXED AND SYNCHRONOUS FLUORESCENCE SPECTROSCOPY

Química Nova ◽  
2021 ◽  
Author(s):  
Marina Freire ◽  
Cristina Gómez ◽  
Anabela Oliveira ◽  
Josino Moreira ◽  
Ana Arias
Keyword(s):  
Fluorescence Spectroscopy ◽  
Synchronous Fluorescence ◽  
Metropolitan Region ◽  
Southeastern Brazil ◽  
Guanabara Bay ◽  
Synchronous Fluorescence Spectroscopy ◽  
Pah Metabolites ◽  
Polycyclic Aromatic ◽  
Pyrene Metabolites

Guanabara Bay (GB) covers the metropolitan region of the state of Rio de Janeiro in Brazil. GB is subject to heavy contamination by polycyclic aromatic hydrocarbons (PAHs), from intense oil activities, which pose an ecotoxicological threat. The aim of this study is to implement and optimize a fluorescence methodology for the determination of biliary PAH metabolites in fish species (burrfish and whitemouth croaker), in order to evaluate biliary PAH metabolites as a biomarker of exposure. Fish were sampled from GB and a control region. Naphthalene, pyrene and benzo(a)pyrene metabolites were determined by Fixed Fluorescence Method (FF), while 1-hydroxypyrene was assessed by Synchronous Fluorescence Spectroscopy (SFS). The implementation and optimization of the FF and SFS methods allowed the determination and evaluation of the exposure of these species to PAHs of pyrogenic and petrogenic origin. Biliary PAH metabolite determinations was proven to be a useful tool for environmental monitoring contamination assessments.

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