Analysis of human colon tissue using Raman spectroscopy

1997 ◽  
Author(s):  
Sarah E. Cope ◽  
Tami L. Freeman ◽  
B. J. Rembacken ◽  
Mark R. Stringer ◽  
David N. Batchelder ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Colon ◽  
Colon Tissue

Ultraviolet Resonance Raman Spectroscopy of Bulk and Microscopic Human Colon Tissue

2000 ◽  
Vol 54 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Nada N. Boustany ◽  
Ramasamy Manoharan ◽  
Ramachandra R. Dasari ◽  
Michael S. Feld
Keyword(s):  
Raman Spectroscopy ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Human Colon ◽  
Colon Tissue ◽  
Ultraviolet Resonance Raman Spectroscopy ◽  
Ultraviolet Resonance Raman

scholarly journals Analysis of Human Colon by Raman Spectroscopy and Imaging-Elucidation of Biochemical Changes in Carcinogenesis

2019 ◽  
Vol 20 (14) ◽  
pp. 3398 ◽  
Author(s):  
Brozek-Pluska ◽  
Musial ◽  
Kordek ◽  
Abramczyk
Keyword(s):  
Raman Spectroscopy ◽  
Human Colon ◽  
Raman Imaging ◽  
Spectroscopy Analysis ◽  
Colon Tissue ◽  
Tissue Samples ◽  
Noncancerous Tissue ◽  
Tumor Mass ◽  
Tumor Tissues ◽  
Safety Margins

Noninvasive Raman imaging of non-fixed and unstained human colon tissues based on vibrational properties of noncancerous and cancerous samples can effectively enable the differentiation between noncancerous and tumor tissues. This work aimed to evaluate the biochemical characteristics of colon cancer and the clinical merits of multivariate Raman image and spectroscopy analysis. Tissue samples were collected during routine surgery. The non-fixed, fresh samples were used to prepare micrometer sections from the tumor mass and the tissue from the safety margins outside of the tumor mass. Adjacent sections were used for typical histological analysis. We have found that the chemical composition identified by Raman spectroscopy of the cancerous and the noncancerous colon samples is sufficiently different to distinguish pathologically changed tissue from noncancerous tissue. We present a detailed analysis of Raman spectra for the human noncancerous and cancerous colon tissue. The multivariate analysis of the intensities of lipids/proteins/carotenoids Raman peaks shows that these classes of compounds can statistically divide analyzed samples into noncancerous and pathological groups, reaffirming that Raman imaging is a powerful technique for the histochemical analysis of human tissues. Raman biomarkers based on ratios for lipids/proteins/carotenoids content were found to be the most useful biomarkers in spectroscopic diagnostics.

scholarly journals Vitamin C − protective role in oxidative stress conditions induced in human normal colon cells by label free Raman spectroscopy and imaging

2021 ◽  
Author(s):  
Karolina Beton ◽  
Beata Brozek-Pluska
Keyword(s):  
Oxidative Stress ◽  
Raman Spectroscopy ◽  
Vitamin C ◽  
Human Colon ◽  
Protective Role ◽  
Spectroscopic Methods ◽  
Label Free ◽  
Normal Cells ◽  
Colon Cells ◽  
Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer, can detect it in advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise and unambiguous differentiation of healthy and cancerous samples. the most important advantage of Raman spectroscopy is ability to identify biomarkers that help in differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for: lipids, proteins, DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS by spectroscopic methods. Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal, ROS injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

Evaluation of ultrasound and glucose synergy effect on the optical clearing and light penetration for human colon tissue using SD-OCT

2014 ◽  
Vol 7 (11-12) ◽  
pp. 938-947 ◽  
Author(s):  
Qingliang Zhao ◽  
Huajiang Wei ◽  
Yonghong He ◽  
Qiushi Ren ◽  
Chuanqing Zhou
Keyword(s):  
Human Colon ◽  
Light Penetration ◽  
Synergy Effect ◽  
Colon Tissue ◽  
Optical Clearing ◽  
Sd Oct

scholarly journals Colonic Saturated Fatty Acid Concentrations and Expression of COX-1, but not Diet, Predict Prostaglandin E2in Normal Human Colon Tissue

2016 ◽  
Vol 68 (7) ◽  
pp. 1192-1201 ◽  
Author(s):  
ElKhansa Sidahmed ◽  
Ananda Sen ◽  
Jianwei Ren ◽  
Arsh Patel ◽  
D. Kim Turgeon ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Saturated Fatty Acid ◽  
Human Colon ◽  
Colon Tissue ◽  
Normal Human ◽  
Cox 1

scholarly journals Label-free quantitative optical assessment of human colon tissue using spatial frequency domain imaging

2018 ◽  
Vol 22 (8) ◽  
pp. 617-621 ◽  
Author(s):  
S. Nandy ◽  
W. Chapman ◽  
R. Rais ◽  
I. González ◽  
D. Chatterjee ◽  
...  
Keyword(s):  
Spatial Frequency ◽  
Frequency Domain ◽  
Human Colon ◽  
Label Free ◽  
Colon Tissue ◽  
Spatial Frequency Domain Imaging ◽  
Spatial Frequency Domain
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