scholarly journals Human colon tissue dissociation for immune cells v1 (protocols.io.tbfeijn)

protocols.io ◽  
2018 ◽  
Author(s):  
Kylie James
Keyword(s):  
Immune Cells ◽  
Human Colon ◽  
Colon Tissue ◽  
Tissue Dissociation

scholarly journals Distinct microbial and immune niches of the human colon

2019 ◽  
Author(s):  
KR James ◽  
T Gomes ◽  
R Elmentaite ◽  
N Kumar ◽  
EL Gulliver ◽  
...  
Keyword(s):  
Immune Cells ◽  
Cell Activation ◽  
T Regulatory Cells ◽  
Bacterial Species ◽  
Human Colon ◽  
Mesenteric Lymph Nodes ◽  
Gastrointestinal Microbiota ◽  
Regional Specificity ◽  
Transcriptional Adaptation ◽  
And Migration

AbstractGastrointestinal microbiota and immune cells interact closely and display regional specificity, but little is known about how these communities differ with location. Here, we simultaneously assess microbiota and single immune cells across the healthy, adult human colon, with paired characterisation of immune cells in the mesenteric lymph nodes, to delineate colonic immune niches at steady-state. We describe distinct T helper cell activation and migration profiles along the colon and characterise the transcriptional adaptation trajectory of T regulatory cells between lymphoid tissue and colon. Finally, we show increasing B cell accumulation, clonal expansion and mutational frequency from caecum to sigmoid colon, and link this to the increasing number of reactive bacterial species.

scholarly journals Correlation Between Immune Lymphoid Cells and Plasmacytoid Dendritic Cells in Human Colon Cancer

2020 ◽  
Author(s):  
Jing Wu ◽  
Hang Cheng ◽  
Tete Li ◽  
Helei Wang ◽  
Guoxia Zang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Dendritic Cells ◽  
Rna Sequencing ◽  
Immune Cells ◽  
Human Colon ◽  
Tumour Microenvironment ◽  
Plasmacytoid Dendritic Cells ◽  
Lymphoid Cells ◽  
Human Colon Cancer

Abstract Background: Innate lymphoid cells (ILCs), so far studied mostly in mouse models, are important tissue-resident innate immune cells that play important roles in the colorectal cancer microenvironment and maintain the mucosal tissue homeostasis. Plasmacytoid dendritic cells (pDCs) present complexity in various tumour types and are correlated with poor prognosis. pDCs can promote HIV-1–induced group 3 ILC (ILC3) depletion through the CD95 pathway. However, the role of ILC3s in human colon cancer and their correlation with other immune cells, especially pDCs, remain unclear. Methods: We characterised ILCs and pDCs in the tumour microenvironment of 58 colon cancer patients by flow cytometry and selected three patients for RNA sequencing. Results: ILC3s were negatively correlated, and pDCs were positively correlated, with cancer pathological grade. There was a negative correlation between the numbers of ILC3s and pDCs in tumour tissues. RNA sequencing confirmed the correlations between ILC3s and pDCs and highlighted the potential function of many ILC- and pDC-associated differentially expressed genes in the regulation of tumour immunity. pDCs can induce apoptosis of ILC3s through the CD95 pathway in the tumour microenvironment. Conclusions: One of the interactions between ILC3s and pDCs is via the CD95 pathway, which may help explain the role of ILC3s in colon cancer.

Evaluation of ultrasound and glucose synergy effect on the optical clearing and light penetration for human colon tissue using SD-OCT

2014 ◽  
Vol 7 (11-12) ◽  
pp. 938-947 ◽  
Author(s):  
Qingliang Zhao ◽  
Huajiang Wei ◽  
Yonghong He ◽  
Qiushi Ren ◽  
Chuanqing Zhou
Keyword(s):  
Human Colon ◽  
Light Penetration ◽  
Synergy Effect ◽  
Colon Tissue ◽  
Optical Clearing ◽  
Sd Oct

scholarly journals Colonic Saturated Fatty Acid Concentrations and Expression of COX-1, but not Diet, Predict Prostaglandin E2in Normal Human Colon Tissue

2016 ◽  
Vol 68 (7) ◽  
pp. 1192-1201 ◽  
Author(s):  
ElKhansa Sidahmed ◽  
Ananda Sen ◽  
Jianwei Ren ◽  
Arsh Patel ◽  
D. Kim Turgeon ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Saturated Fatty Acid ◽  
Human Colon ◽  
Colon Tissue ◽  
Normal Human ◽  
Cox 1

scholarly journals Label-free quantitative optical assessment of human colon tissue using spatial frequency domain imaging

2018 ◽  
Vol 22 (8) ◽  
pp. 617-621 ◽  
Author(s):  
S. Nandy ◽  
W. Chapman ◽  
R. Rais ◽  
I. González ◽  
D. Chatterjee ◽  
...  
Keyword(s):  
Spatial Frequency ◽  
Frequency Domain ◽  
Human Colon ◽  
Label Free ◽  
Colon Tissue ◽  
Spatial Frequency Domain Imaging ◽  
Spatial Frequency Domain

scholarly journals Biological Responses of Onion-Shaped Carbon Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1016 ◽  
Author(s):  
Jaehee Jang ◽  
Youngjun Kim ◽  
Jangsun Hwang ◽  
Yonghyun Choi ◽  
Masayoshi Tanaka ◽  
...  
Keyword(s):  
Immune Cells ◽  
Carbon Nanoparticles ◽  
Carbon Nanomaterials ◽  
Human Colon ◽  
Oxygen Species ◽  
Biological Applications ◽  
Biotechnological Applications ◽  
Animal Cells ◽  
Biological Responses ◽  
Human Immune

Nanodiamonds are emerging as new nanoscale materials because of their chemical stability, excellent crystallinity, and unique optical properties. In this study, the structure of nanodiamonds was engineered to produce carbon nano-onion particles (CNOs) with multiple layers. Following a series of physicochemical characterizations of the CNOs, various evaluations for biological responses were conducted for potential biotechnological applications of the CNOs. The possibility of biological applications was first confirmed by assessment of toxicity to animal cells, evaluation of hemolysis reactions, and evaluation of reactive oxygen species. In addition, human immune cells were evaluated for any possible induction of an immune response by CNOs. Finally, the toxicity of CNOs to Escherichia coli present in the human colon was evaluated. CNOs have the chemical and physical properties to be a unique variety of carbon nanomaterials, and their toxicity to animal and human cells is sufficiently low that their biotechnological applications in the future are expected.

Abstract 657: The comparison of calcium-induced differentiation in colonoid cultures derived from the normal human colon tissue and colonic adenomas

2019 ◽  
Author(s):  
Muhammad N. Aslam ◽  
Shannon D. McClintock ◽  
Areeba H. Rizvi ◽  
Durga Attili ◽  
Shailja Pandya ◽  
...  
Keyword(s):  
Human Colon ◽  
Colon Tissue ◽  
Induced Differentiation ◽  
Colonic Adenomas ◽  
Normal Human
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