Three-dimensional image modeling based on least squares fitting by using adaptive subdivision of a tetrahedron

Author(s):  
Kun Lee ◽  
Oubong Gwun
Author(s):  
Craig M. Shakarji ◽  
Vijay Srinivasan

This paper addresses the combinatorial characterizations of the optimality conditions for constrained least-squares fitting of circles, cylinders, and spheres to a set of input points. It is shown that the necessary condition for optimization requires contacting at least two input points. It is also shown that there exist cases where the optimal condition is achieved while contacting only two input points. These problems arise in digital manufacturing, where one is confronted with the task of processing a (potentially large) number of points with three-dimensional coordinates to establish datums on manufactured parts. The optimality conditions reported in this paper provide the necessary conditions to verify if a candidate solution is feasible, and to design new algorithms to compute globally optimal solutions.


Author(s):  
Craig M. Shakarji ◽  
Vijay Srinivasan

This paper addresses the combinatorial characterizations of the optimality conditions for constrained least-squares fitting of circles, cylinders, and spheres to a set of input points. It is shown that the necessary condition for optimization requires contacting at least two input points. It is also shown that there exist cases where the optimal condition is achieved while contacting only two input points. These problems arise in digital manufacturing, where one is confronted with the task of processing a (potentially large) number of points with three-dimensional coordinates to establish datums on manufactured parts. The optimality conditions reported in this paper provide the necessary conditions to verify if a candidate solution is feasible, and to design new algorithms to compute globally optimal solutions.


2017 ◽  
Vol 890 ◽  
pp. 012121
Author(s):  
Elvira Rahmadiantri ◽  
Made Putri Lawiyuniarti ◽  
Intan Muchtadi-Alamsyah ◽  
Gantina Rachmaputri

2019 ◽  
Author(s):  
Camille Bayas ◽  
Alex von Diezmann ◽  
Anna-Karin Gustavsson ◽  
W. E. Moerner

Abstract Automated processing of double-helix (DH) microscope images of fluorescent single molecules (SMs) streamlines the protocol required to obtain super-resolved three-dimensional (3D) reconstructions of ultrastructures in biological samples by single-molecule active control microscopy (SMACM). Here, we present a suite of MATLAB subroutines, bundled with an easy-to-use graphical user interface (GUI), that facilitates 3D localization of single emitters (e.g. SMs, fluorescent beads, or quantum dots) with typical precisions of tens of nanometers in multi-frame movies acquired using a wide-field DH epifluorescence microscope. The algorithmic approach is based upon template matching for SM recognition and least‑squares fitting for 3D position measurement, both of which are computationally expedient and precise. Overlapping images of SMs are ignored, and the precision of least-squares fitting is not as high as maximum likelihood-based methods. Version 2.0 of Easy-DHPSF augments the approach of Version 1.0 by incorporating code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function.


2019 ◽  
Author(s):  
Camille Bayas ◽  
Alex von Diezmann ◽  
Anna-Karin Gustavsson ◽  
W. E. Moerner

Abstract Automated processing of double-helix (DH) microscope images of fluorescent single molecules (SMs) streamlines the protocol required to obtain super-resolved three-dimensional (3D) reconstructions of ultrastructures in biological samples by single-molecule active control microscopy (SMACM). Here, we present a suite of MATLAB subroutines, bundled with an easy-to-use graphical user interface (GUI), that facilitates 3D localization of single emitters (e.g. SMs, fluorescent beads, or quantum dots) with typical precisions of tens of nanometers in multi-frame movies acquired using a wide-field DH epifluorescence microscope. The algorithmic approach is based upon template matching for SM recognition and least‑squares fitting for 3D position measurement, both of which are computationally expedient and precise. Overlapping images of SMs are ignored, and the precision of least-squares fitting is not as high as maximum likelihood-based methods. Version 2.0 of Easy-DHPSF augments the approach of Version 1.0 by incorporating code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function.


2014 ◽  
Vol 8 ◽  
pp. 7409-7421 ◽  
Author(s):  
Alexandra Malyugina ◽  
Konstantin Igudesman ◽  
Dmitry Chickrin

Author(s):  
R. A. Crowther

The reconstruction of a three-dimensional image of a specimen from a set of electron micrographs reduces, under certain assumptions about the imaging process in the microscope, to the mathematical problem of reconstructing a density distribution from a set of its plane projections.In the absence of noise we can formulate a purely geometrical criterion, which, for a general object, fixes the resolution attainable from a given finite number of views in terms of the size of the object. For simplicity we take the ideal case of projections collected by a series of m equally spaced tilts about a single axis.


2011 ◽  
Vol 131 (2) ◽  
pp. 320-328 ◽  
Author(s):  
Cunwei Lu ◽  
Hiroya Kamitomo ◽  
Ke Sun ◽  
Kazuhiro Tsujino ◽  
Genki Cho

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