Heavy-atom modified near-IR fluorescent dyes for DNA sequencing applications: synthesis and photophysical characterization

1997 ◽  
Author(s):  
James H. Flanagan, Jr. ◽  
Sarah E. Romero ◽  
Benjamin L. Legendre, Jr. ◽  
Robert P. Hammer ◽  
Steven A. Soper
Keyword(s):  
Dna Sequencing ◽  
Fluorescent Dyes ◽  
Heavy Atom ◽  
Near Ir

Near-Infrared Heavy-Atom-Modified Fluorescent Dyes for Base-Calling in DNA-Sequencing Applications Using Temporal Discrimination

1998 ◽  
Vol 70 (13) ◽  
pp. 2676-2684 ◽  
Author(s):  
James H. Flanagan, ◽  
Clyde V. Owens ◽  
Sarah E. Romero ◽  
Emanuel Waddell ◽  
Shaheer H. Kahn ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Near Infrared ◽  
Fluorescent Dyes ◽  
Temporal Discrimination ◽  
Heavy Atom ◽  
Base Calling

Single-lane single-fluor sequencing using dideoxy-labeled, heavy-atom-modified near-IR fluorescent dyes

1995 ◽  
Author(s):  
Daryl C. Williams ◽  
James H. Flanagan, Jr. ◽  
Benjamin L. Legendre, Jr. ◽  
Robert P. Hammer ◽  
Steven A. Soper
Keyword(s):  
Fluorescent Dyes ◽  
Heavy Atom ◽  
Near Ir

Chemistry at Boron: Synthesis and Properties of Red to Near-IR Fluorescent Dyes Based on Boron-Substituted Diisoindolomethene Frameworks

2011 ◽  
Vol 76 (11) ◽  
pp. 4489-4505 ◽  
Author(s):  
Gilles Ulrich ◽  
Sébastien Goeb ◽  
Antoinette De Nicola ◽  
Pascal Retailleau ◽  
Raymond Ziessel
Keyword(s):  
Fluorescent Dyes ◽  
Near Ir

Electrophoretically Uniform Fluorescent Dyes for Automated DNA Sequencing

Science ◽  
1996 ◽  
Vol 271 (5254) ◽  
pp. 1420-1422 ◽  
Author(s):  
M. L. Metzker ◽  
J. Lu ◽  
R. A. Gibbs
Keyword(s):  
Dna Sequencing ◽  
Fluorescent Dyes ◽  
Automated Dna Sequencing

A review of DNA sequencing techniques

2002 ◽  
Vol 35 (2) ◽  
pp. 169-200 ◽  
Author(s):  
Lilian T. C. França ◽  
Emanuel Carrilho ◽  
Tarso B. L. Kist
Keyword(s):  
Dna Sequencing ◽  
Real Time ◽  
Single Molecule ◽  
Fluorescent Dyes ◽  
Read Length ◽  
Chemical Methods ◽  
Single Molecule Sequencing ◽  
Exonuclease Digestion ◽  
Speed Accuracy ◽  
Sanger Method

1. Summary 1692. Introduction 1703. Sanger's method and other enzymic methods 1703.1 Random approach 1713.2 Direct approach 1713.3 Enzyme technology 1753.4 Sample preparation 1753.5 Labels and DNA labelling 1763.5.1 Radioisotopes 1763.5.2 Chemiluminescent detection 1763.5.3 Fluorescent dyes 1773.6 Fragment separation and analysis 1803.6.1 Electrophoresis 1803.6.2 Mass spectrometry – an alternative 1824. Maxam & Gilbert and other chemical methods 1835. Pyrosequencing – DNA sequencing in real time by the detection of released PPi 1876. Single molecule sequencing with exonuclease 1907. Conclusion 1928. Acknowledgements 1929. References 193The four best known DNA sequencing techniques are reviewed. Important practical issues covered are read-length, speed, accuracy, throughput, cost, as well as the automation of sample handling and preparation. The methods reviewed are: (i) the Sanger method and its most important variants (enzymic methods); (ii) the Maxam & Gilbert method and other chemical methods; (iii) the PyrosequencingTM method – DNA sequencing in real time by the detection of released pyrophosphate (PPi); and (iv) single molecule sequencing with exonuclease (exonuclease digestion of a single molecule composed of a single strand of fluorescently labelled deoxynucleotides). Each method is briefly described, the current literature is covered, advantages, disadvantages, and the most suitable applications of each method are discussed.

scholarly journals Improved DNA Sequencing Accuracy and Detection of Heterozygous Alleles Using Manganese Citrate and Different Fluorescent Dye Terminators

1999 ◽  
Vol 9 (6) ◽  
pp. 588-595
Author(s):  
Christopher Korch ◽  
Harry Drabkin
Keyword(s):  
Dna Sequencing ◽  
Fluorescent Dyes ◽  
Fluorescent Dye ◽  
Allele Detection ◽  
Base Calling ◽  
Automated Dna Sequencing ◽  
Dye Terminators ◽  
Rhodamine Dyes

The use of dideoxynucleotide triphosphates labeled with different fluorescent dyes (dye terminators) is the most versatile method for automated DNA sequencing. However, variation in peak heights reduces base-calling accuracy and limits heterozygous allele detection, favoring use of dye-labeled primers for this purpose. We have discovered that the addition of a manganese salt to the PE Applied Biosystems dye-terminator sequencing kits overcomes these limitations for the older rhodamine dyes as well as the more recent dichloro-rhodamine dyes (dRhodamine and BigDyes). Addition of manganese to reactions containing dRhodamine-based dye terminators produced the highest base-calling accuracy. This combination resulted in the most uniform electropherogram profiles, superior to those produced by BigDye terminators and published for dye primers, and facilitated detection of heterozygous alleles.

scholarly journals Activating Room-Temperature Phosphorescence of Organic Luminophores via External Heavy-Atom Effect and Rigidity of Ionic Polymer Matrix

2021 ◽  
Author(s):  
Xiang Ma ◽  
Zi-Ang Yan ◽  
Xiaohan Lin ◽  
Siyu Sun ◽  
He Tian
Keyword(s):  
Polymer Matrix ◽  
Room Temperature ◽  
Fluorescent Dyes ◽  
Amorphous Polymer ◽  
Heavy Atom ◽  
General Strategy ◽  
Triplet States ◽  
Room Temperature Phosphorescence ◽  
Heavy Atom Effect ◽  
Atom Effect

Pure organic room-temperature phosphorescence (RTP) materials have attracted wide attention for their easy preparation, low toxicity and applications in professional fields such as bioimaging and anti-counterfeiting. Developing phosphorescent systems with more universality and less difficulty in synthesis has long been the pursuit of materials scientists. By employing polymeric quaternary ammonium salt with an ionic bonding matrix and heavy atoms, commercial fluorescent dyes are directly endowed with phosphorescence emission. In a single amorphous polymer, the external heavy-atom effect generates excited triplet states, which are further stabilized by the rigid polymer matrix. This study proposed a new general strategy to design and develop pure organic RTP materials starting from the vast library of organic dyes without complicated chemical synthesis.

scholarly journals Near IR excitation of heavy atom free Bodipy photosensitizers through the intermediacy of upconverting nanoparticles

2014 ◽  
Vol 50 (64) ◽  
pp. 8896-8899 ◽  
Author(s):  
Seda Demirel Topel ◽  
Gunseli Turgut Cin ◽  
Engin U. Akkaya
Keyword(s):  
Singlet Oxygen ◽  
Heavy Atom ◽  
Near Ir

Orthogonal dimers of Bodipy can be effectively excited to generate singlet oxygen at 980 nm.

Simultaneous On-line DNA Sequencing on Both Strands with Two Fluorescent Dyes

1995 ◽  
Vol 224 (1) ◽  
pp. 117-121 ◽  
Author(s):  
S. Wiemann ◽  
J. Stegemann ◽  
D. Grothues ◽  
A. Bosch ◽  
X. Estivill ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Fluorescent Dyes ◽  
On Line
Sign in / Sign up

Export Citation Format

Share Document