Autofluorescence of human epidermis under UV irradiation in vitro

1997 ◽  
Author(s):  
Svetlana P. Chernova ◽  
Alexander B. Pravdin ◽  
E. V. Bukatova
Keyword(s):  
Uv Irradiation ◽  
Human Epidermis

scholarly journals Bleaching of chlorophylls by UV irradiation in vitro: The effects on chlorophyll organization in acetone and n-hexane

2008 ◽  
Vol 73 (3) ◽  
pp. 271-282 ◽  
Author(s):  
Jelena Zvezdanovic ◽  
Dejan Markovic
Keyword(s):  
Uv Irradiation ◽  
Photosynthetic Pigments ◽  
Energy Input ◽  
First Order ◽  
First Order Kinetics ◽  
Uv Photons ◽  
The Stability ◽  
Major Factors

The stability of chlorophylls toward UV irradiation was studied by Vis spectrophotometry in extracts containing mixtures of photosynthetic pigments in acetone and n-hexane. The chlorophylls underwent destruction (bleaching) obeying first-order kinetics. The bleaching was governed by three major factors: the energy input of the UV photons, the concentration of the chlorophylls and the polarity of the solvent, implying different molecular organizations of the chlorophylls in the two solvents.

Initial results with EPI-201, a developing in vitro model of human epidermis

1998 ◽  
Vol 16 ◽  
pp. S86
Author(s):  
Mitchell Klausner ◽  
Patricia Neal ◽  
Joseph Kubilus
Keyword(s):  
In Vitro Model ◽  
Human Epidermis ◽  
Vitro Model ◽  
Initial Results

Reconstruction of Human Epidermis In Vitro

2003 ◽  
pp. 353-360
Author(s):  
Marie-Cbcile Lenoir-Viale
Keyword(s):  
Human Epidermis

scholarly journals Nanostructured Lipid Carrier Gel for the Dermal Application of Lidocaine: Comparison of Skin Penetration Testing Methods

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 310 ◽  
Author(s):  
Stella Zsikó ◽  
Kendra Cutcher ◽  
Anita Kovács ◽  
Mária Budai-Szűcs ◽  
Attila Gácsi ◽  
...  
Keyword(s):  
Drug Release ◽  
Human Skin ◽  
Ex Vivo ◽  
Study Drug ◽  
Skin Penetration ◽  
Human Epidermis ◽  
Nanostructured Lipid Carrier ◽  
Experimental Findings

The aim of this research was to investigate the stability of a lidocaine-loaded nanostructured lipid carrier dispersion at different temperatures, formulate a nanostructured lipid carrier gel, and test the penetration profile of lidocaine from the nanostructured lipid carrier gel using different skin penetration modeling methods. The formulations were characterized by laser diffraction, rheological measurements and microscopic examinations. Various in vitro methods were used to study drug release, diffusion and penetration. Two types of vertical Franz diffusion cells with three different membranes, including cellulose, Strat-M®, and heat separated human epidermis were used and compared to the Skin-parallel artificial membrane permeability assay (PAMPA) method. Results indicated that the nanostructured lipid carrier dispersion had to be gelified as soon as possible for proper stability. Both the Skin-PAMPA model and Strat-M® membranes correlated favorably with heat separated human epidermis in this research, with the Strat-M® membranes sharing the most similar drug permeability profile to an ex vivo human skin model. Our experimental findings suggest that even when the best available in vitro experiment is selected for modeling human skin penetration to study nanostructured lipid carrier gel systems, relevant in vitro/in vivo correlation should be made to calculate the drug release/permeation in vivo. Future investigations in this field are still needed to demonstrate the influence of membranes and equipment from other classes on other drug candidates.

scholarly journals A new reconstructed human epidermis for in vitro skin irritation testing

2017 ◽  
Vol 42 ◽  
pp. 31-37 ◽  
Author(s):  
Tatiana do Nascimento Pedrosa ◽  
Carolina Motter Catarino ◽  
Paula Comune Pennacchi ◽  
Sílvia Romano de Assis ◽  
Fabrícia Gimenes ◽  
...  
Keyword(s):  
Skin Irritation ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis
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