Quantitative absorption imaging of single nanoparticles by widefield interferometric photothermal microscopy

2021 ◽  
Author(s):  
Yu-Chien Huang ◽  
Te-Hsin Chen ◽  
Jz-Yuan Juo ◽  
Shi-Wei Chu ◽  
Chia-Lung Hsieh
Keyword(s):  
Absorption Imaging ◽  
Photothermal Microscopy ◽  
Quantitative Absorption

scholarly journals Quantitative absorption imaging of red blood cells to determine physical and mechanical properties

RSC Advances ◽  
2020 ◽  
Vol 10 (64) ◽  
pp. 38923-38936
Author(s):  
Ratul Paul ◽  
Yuyuan Zhou ◽  
Mehdi Nikfar ◽  
Meghdad Razizadeh ◽  
Yaling Liu
Keyword(s):  
Mechanical Properties ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Microfluidic Channel ◽  
Physical And Mechanical Properties ◽  
Constant Thickness ◽  
Absorption Imaging ◽  
Quantitative Absorption

The constant thickness in the microfluidic channel is used for controlled absorption of red and blue light to measure red blood cell hemoglobin and height mapping. High speed recording of the height mapping provides us the membrane fluctuation.

Antibody Binding to Membrane of Cultured Melanoma Cells by Sera of Melanoma Patients

1979 ◽  
Vol 65 (1) ◽  
pp. 51-64 ◽  
Author(s):  
Silvana Canevari ◽  
Giuseppe Fossati ◽  
Paolo Vezzoni ◽  
Sergio Biguzzi ◽  
Jose Garcia-Puche ◽  
...  
Keyword(s):  
Binding Activity ◽  
Absorption Capacity ◽  
Target Cells ◽  
Similar Amount ◽  
Experimental Conditions ◽  
Healthy Donors ◽  
Fetal Bovine ◽  
Melanoma Patients ◽  
Quantitative Absorption ◽  
Sepharose 4B

One hundred and nine sera from 75 patients with malignant melanoma and 69 sera from as many healthy donors were assayed by isotopic antiglobulin technique (IAT) on 2 melanoma cell lines. The same picture of reactivity was observed with patients’ and healthy donors’ sera, and in both groups 35% of the cases were high responders on 1 line and 21% on the other one. The specificity of the reactions was analyzed by absorption experiments using 12 melanoma sera selected for their high binding activity. Pools of human erythrocytes or leukocytes did not remove, except in 1 case respectively, the activity of the sera, suggesting that it was not directed against alloantigens. Quantitative absorption experiments were done with the 2 melanoma lines and with 1 colon carcinoma line. The results, evaluated on the basis of absorption capacity per cell, indicate that the 2 melanoma lines had a similar amount of shared antigens, whereas the colon line was also effective in absorbing out the serum activity, but less frequently and less efficiently. Further experiments performed to analyze the influence of culturing the target cells in presence of fetal bovine serum (FBS), showed that the activity of sera was removed, at various degrees for different sera, by absorption with free FBS, with FBS coupled to Sepharose 4B, and with normal leukocytes cultured overnight with 10% FBS. The same positive melanoma sera became negative when assayed on the same melanoma line cultured in γ-globulin-depleted human AB serum. In conclusion, in our experimental conditions, the activity of melanoma sera seems mostly directed against components of FBS absorbed on cell membrane during culturing.

Photothermal and Heat-Transfer Properties of Aqueous Detonation Nanodiamonds by Photothermal Microscopy and Transient Spectroscopy

2021 ◽  
Vol 125 (14) ◽  
pp. 7808-7823
Author(s):  
Mikhail A. Proskurnin ◽  
Liliya O. Usoltseva ◽  
Dmitry S. Volkov ◽  
Dmitry A. Nedosekin ◽  
Mikhail V. Korobov ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Detonation Nanodiamonds ◽  
Photothermal Microscopy ◽  
Transient Spectroscopy ◽  
Transfer Properties

On the quantitative absorption and Stokes shift in PbSe quantum dots embedded in glasses

2014 ◽  
Vol 118 (1) ◽  
pp. 85-92
Author(s):  
De-Wei Ma ◽  
Hui-Lv Jiang ◽  
Cheng Cheng
Keyword(s):  
Quantum Dots ◽  
Stokes Shift ◽  
Quantitative Absorption

Adaptive telescope for confocal photothermal microscopy of irregular surfaces

2021 ◽  
Vol 60 (04) ◽  
Author(s):  
Luis Emiliano Jan ◽  
Facundo Zaldivar Escola ◽  
Nelida Mingolo
Keyword(s):  
Irregular Surfaces ◽  
Photothermal Microscopy

Infrared Absorption Imaging of Water Ingress Into the Encapsulation of (Opto-)Electronic Devices

2019 ◽  
Vol 9 (1) ◽  
pp. 252-258 ◽  
Author(s):  
Johannes Hepp ◽  
Andreas Vetter ◽  
Stefan Langner ◽  
Michael Woiton ◽  
Gordana Jovicic ◽  
...  
Keyword(s):  
Infrared Absorption ◽  
Electronic Devices ◽  
Water Ingress ◽  
Absorption Imaging

Specific Near-IR Absorption Imaging of Glioblastomas Using Integrin-Targeting Gold Nanorods

2011 ◽  
Vol 21 (6) ◽  
pp. 1082-1088 ◽  
Author(s):  
Jihye Choi ◽  
Jaemoon Yang ◽  
Joseph Park ◽  
Eunjung Kim ◽  
Jin-Suck Suh ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Ir Absorption ◽  
Absorption Imaging ◽  
Near Ir
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