scholarly journals Quantitative absorption imaging of red blood cells to determine physical and mechanical properties

RSC Advances ◽  
2020 ◽  
Vol 10 (64) ◽  
pp. 38923-38936
Author(s):  
Ratul Paul ◽  
Yuyuan Zhou ◽  
Mehdi Nikfar ◽  
Meghdad Razizadeh ◽  
Yaling Liu
Keyword(s):  
Mechanical Properties ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Microfluidic Channel ◽  
Physical And Mechanical Properties ◽  
Constant Thickness ◽  
Absorption Imaging ◽  
Quantitative Absorption

The constant thickness in the microfluidic channel is used for controlled absorption of red and blue light to measure red blood cell hemoglobin and height mapping. High speed recording of the height mapping provides us the membrane fluctuation.

Study on the Turbulent Injury Principle of Blood in the High-Speed Spiral Blood Pump

2011 ◽  
Vol 393-395 ◽  
pp. 992-995
Author(s):  
Zhong Yun ◽  
Chuang Xiang ◽  
Xiao Yan Tang ◽  
Fen Shi
Keyword(s):  
Shear Stress ◽  
Flow Field ◽  
Blood Cell ◽  
Turbulent Flow ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Internal Flow ◽  
Blood Pump ◽  
Turbulent Flow Field

The strongly swirling turbulent flow in the internal flow field of a high-speed spiral blood pump(HSBP), is one of important factors leading to the fragmentation of the red blood cell(RBC) and the hemolysis. The study on the turbulent injure principle of blood in the HSBP is carried out by using the theory of waterpower rotated flow field and the hemorheology. The numerical equation of the strongly swirling turbulent flow field is proposed. The largest stable diameter of red blood cells in the turbulent flow field is analyzed. The determinant gist on the red blood cell turbulent fragmentation is obtained. The results indicate that in the HSMP, when turbulent flow is more powerful, shear stress is weaker, the vortex mass with energy in flow field may cause serious turbulent fragmentation because of the diameter which is smaller than the RBC’s. The RBC’s turbulent breakage will occur when the Weber value is larger than 12.

scholarly journals Microfluidic Obstacle Arrays Induce Large Reversible Shape Change in Red Blood Cells

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 783
Author(s):  
David W. Inglis ◽  
Robert E. Nordon ◽  
Jason P. Beech ◽  
Gary Rosengarten
Keyword(s):  
Shear Stress ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Shape Change ◽  
Maximum Shear Stress ◽  
Flow Rates ◽  
Degree Of Deformation

Red blood cell (RBC) shape change under static and dynamic shear stress has been a source of interest for at least 50 years. High-speed time-lapse microscopy was used to observe the rate of deformation and relaxation when RBCs are subjected to periodic shear stress and deformation forces as they pass through an obstacle. We show that red blood cells are reversibly deformed and take on characteristic shapes not previously seen in physiological buffers when the maximum shear stress was between 2.2 and 25 Pa (strain rate 2200 to 25,000 s−1). We quantify the rates of RBC deformation and recovery using Kaplan–Meier survival analysis. The time to deformation decreased from 320 to 23 milliseconds with increasing flow rates, but the distance traveled before deformation changed little. Shape recovery, a measure of degree of deformation, takes tens of milliseconds at the lowest flow rates and reached saturation at 2.4 s at a shear stress of 11.2 Pa indicating a maximum degree of deformation was reached. The rates and types of deformation have relevance in red blood cell disorders and in blood cell behavior in microfluidic devices.

Image Analysis of Human Nailfold Capillary With High Speed Digital Video Capillaroscopy

2007 ◽  
Author(s):  
H. Kuroda ◽  
M. Iribe ◽  
M. Matsubara ◽  
M. Watanabe ◽  
T. Sanada
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Digital Video ◽  
Video Camera ◽  
Center Line ◽  
Digital Video Camera ◽  
Cell Velocity ◽  
Red Blood Cell Velocity

Diagnoses of skin diseases are considerably difficult tasks due to the multiply-folded factors. Nailfold capillaroscopy has been developed to diagnosis microvascular disturbances mainly in connective tissue diseases, including systemic sclerosis, dermatomyositis, systemic lupus erythematosus, and Raynaud’s phenomenon. Capillaroscopy is non-invasive, easy to use, low cost and suitable for observation of these typical phenomena. We improved conventional capillaroscopy by constructing “high speed digital video capillaroscopy”, by integrating high speed digital video camera, deep-focus zoom lens, appropriate light source and light collecting adaptor. High speed digital video camera enabled us to observe the individual red blood cell in human nailfold capillary in vivo. The light collecting adaptor is effective for preventing skin from excessive light exposure, which causes serious damage. The first objective of this study is to extract the shape of nailfold capillary quantitatively by using binarization and the level-set method. By using the level-set method, the function, which distinguishes outside from inside of the capillary and also evaluates radius distribution along the capillary center line, is calculated. Based on this mathematical description of capillary shape, more rigorous definition of the capillary red blood cell velocity than the conventional method is obtained. The second objective of this study is to propose the innovative measurement method of red blood cell velocity in nailfold capillary. As plasma gaps show high brightness we trace them and estimate the velocities of blood cells on the center line of capillary. The last objective of this study is to observe the behavior of red blood cell. We evaluate the movement of individual red blood cell, not only in the axial direction but also the lateral direction. We analyze the series of images of red blood cells in capillary and discuss their behavior.

scholarly journals Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 358
Author(s):  
Ann-Kathrin Reichenwallner ◽  
Esma Vurmaz ◽  
Kristina Battis ◽  
Laura Handl ◽  
Helin Üstün ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Count ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Microfluidic Channel ◽  
Hemoglobin Concentration ◽  
Digital Holographic Microscopy ◽  
Optical Investigation ◽  
Cell Counts

We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of −0.26×106 μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts.

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

Microfluidic electrical impedance assessment of red blood cell mediated microvascular occlusion

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yuncheng Man ◽  
Debnath Maji ◽  
Ran An ◽  
Sanjay Ahuja ◽  
Jane A Little ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Electrical Impedance ◽  
Cell Disease ◽  
Blood Disorders

Alterations in the deformability of red blood cells (RBCs), occurring in hemolytic blood disorders such as sickle cell disease (SCD), contributes to vaso-occlusion and disease pathophysiology. However, there are few...

Reorientation of a single red blood cell during sedimentation

2016 ◽  
Vol 806 ◽  
pp. 102-128 ◽  
Author(s):  
D. Matsunaga ◽  
Y. Imai ◽  
C. Wagner ◽  
T. Ishikawa
Keyword(s):  
Angular Velocity ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Driving Force ◽  
Viscosity Ratio ◽  
Orientation Angle ◽  
Bond Number ◽  
Vertical Orientation ◽  
Main Factors

The reorientation phenomenon of a single red blood cell during sedimentation is simulated using the boundary element method. The cell settles downwards due to a density difference between the internal and external fluids, and it changes orientation toward a vertical orientation regardless of Bond number or viscosity ratio. The reorientation phenomenon is explained by a shape asymmetry caused by the gravitational driving force, and the shape asymmetry increases almost linearly with the Bond number. When velocities are normalised by the driving force, settling/drifting velocities are weak functions of the Bond number and the viscosity ratio, while the angular velocity of the reorientation drastically changes with these parameters: the angular velocity is smaller for lower Bond number or higher viscosity ratio. As a consequence, trajectories of the sedimentation are also affected by the angular velocity, and blood cells with slower reorientation travel longer distances in the drifting direction. We also explain the mechanism of the reorientation using an asymmetric dumbbell. From the analysis, we show that the magnitude of the angular velocity is explained by two main factors: the shape asymmetry and the instantaneous orientation angle.

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