SPAD array technology enables fluctuation-contrast super-resolution in a confocal microscope

2021 ◽  
Author(s):  
Ron Tenne ◽  
Adrian Makowski ◽  
Gur Lubin ◽  
Michel Antolovic ◽  
Uri Rossman ◽  
...  
Keyword(s):  
Super Resolution ◽  
Confocal Microscope ◽  
Array Technology

scholarly journals Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration

1998 ◽  
Vol 84 (8) ◽  
pp. 4033-4042 ◽  
Author(s):  
M. Schrader ◽  
S. W. Hell ◽  
H. T. M. van der Voort
Keyword(s):  
Image Restoration ◽  
Three Dimensional ◽  
Super Resolution ◽  
Confocal Microscope

scholarly journals Versatile, do-it-yourself, low-cost spinning disk confocal microscope

2021 ◽  
Author(s):  
Aaron R. Halpern ◽  
Min Yen Lee ◽  
Marco D. Howard ◽  
Marcus A. Woodworth ◽  
Philip R. Nicovich ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Single Molecule ◽  
Low Cost ◽  
Super Resolution ◽  
Confocal Microscope ◽  
Spinning Disk ◽  
Invaluable Tool ◽  
Do It Yourself ◽  
Conventional Microscopy ◽  
Core Facilities

Confocal microscopy is an invaluable tool for 3D imaging of biological specimens, however, accessibility is often limited to core facilities due to the high cost of the hardware. We describe an inexpensive do-it-yourself (DIY) spinning disk confocal microscope (SDCM) module based on a commercially fabricated chromium photomask that can be added on to a laser-illuminated epifluorescence microscope. The SDCM achieves strong performance across a wide wavelength range (~400-800 nm) as demonstrated through a series of biological imaging applications that include conventional microscopy (immunofluorescence, small-molecule stains, and fluorescence in situ hybridization) and super-resolution microscopy (single-molecule localization microscopy and expansion microscopy). This low-cost and simple DIY SDCM is well-documented and should help increase accessibility to confocal microscopy for researchers.

Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters (Conference Presentation)

2020 ◽  
Author(s):  
Denitza Denkova ◽  
Martin Ploschner ◽  
Minakshi Das ◽  
Lindsay M. Parker ◽  
Xianlin Zheng ◽  
...  
Keyword(s):  
Super Resolution ◽  
Confocal Microscope

Axial super-resolution using a double-zone pinhole in a confocal microscope

2019 ◽  
Vol 45 (1) ◽  
pp. 232
Author(s):  
Xiangdong Huang ◽  
Jiubin Tan
Keyword(s):  
Super Resolution ◽  
Confocal Microscope

scholarly journals Super-resolution for everybody: An image processing workflow to obtain high-resolution images with a standard confocal microscope

Methods ◽  
2017 ◽  
Vol 115 ◽  
pp. 17-27 ◽  
Author(s):  
France Lam ◽  
Damien Cladière ◽  
Cyndélia Guillaume ◽  
Katja Wassmann ◽  
Susanne Bolte
Keyword(s):  
Image Processing ◽  
High Resolution ◽  
Super Resolution ◽  
Confocal Microscope ◽  
High Resolution Images

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

A new miniaturized confocal microscope allows immediate in vivo histology of different organs

2005 ◽  
Vol 43 (05) ◽  
Author(s):  
M Goetz ◽  
MF Neurath ◽  
P Delaney ◽  
S Gregor ◽  
D Strand ◽  
...  
Keyword(s):  
Confocal Microscope

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy
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