Microscopic energy transfer spectroscopy to determine mitochondrial malfunction in human myotubes

1996 ◽  
Author(s):  
Michael H. Gschwend ◽  
Wolfgang S. L. Strauss ◽  
H. Brinkmeier ◽  
R. Ruedel ◽  
Rudolf W. Steiner ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Human Myotubes ◽  
Microscopic Energy

Time-gated microscopic energy transfer measurements for probing mitochondrial metabolism

1997 ◽  
Vol 7 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Herbert Schneckenburger ◽  
Michael H. Gschwend ◽  
Wolfgang S. L. Strauss ◽  
Reinhard Sailer ◽  
Rudolf Steiner
Keyword(s):  
Energy Transfer ◽  
Mitochondrial Metabolism ◽  
Microscopic Energy

A quantitative approach to inner shell losses

1978 ◽  
Vol 36 (1) ◽  
pp. 526-527 ◽  
Author(s):  
R.D. Leapman ◽  
P. Rez ◽  
D.F. Mayers
Keyword(s):  
Energy Transfer ◽  
Cross Section ◽  
Cross Sections ◽  
Accurate Determination ◽  
Background Intensity ◽  
Core Excitation ◽  
Quantitative Basis ◽  
Cross Section Differential ◽  
Bound Electrons

Microanalysis by EELS has been developing rapidly and though the general form of the spectrum is now understood there is a need to put the technique on a more quantitative basis (1,2). Certain aspects important for microanalysis include: (i) accurate determination of the partial cross sections, σx(α,ΔE) for core excitation when scattering lies inside collection angle a and energy range ΔE above the edge, (ii) behavior of the background intensity due to excitation of less strongly bound electrons, necessary for extrapolation beneath the signal of interest, (iii) departures from the simple hydrogenic K-edge seen in L and M losses, effecting σx and complicating microanalysis. Such problems might be approached empirically but here we describe how computation can elucidate the spectrum shape.The inelastic cross section differential with respect to energy transfer E and momentum transfer q for electrons of energy E0 and velocity v can be written as

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