A high-throughput Fourier-transform wide-field hyperspectral microscope for fluorescence and Raman imaging

2021 ◽  
Author(s):  
Cristian Manzoni ◽  
Daniela Comelli ◽  
Giulio N. Cerullo ◽  
Benedetto Ardini ◽  
Renzo Vanna ◽  
...  
Keyword(s):  
Fourier Transform ◽  
High Throughput ◽  
Raman Imaging ◽  
Wide Field

An ultrastable Fourier-transform wide-field hyperspectral microscope for fluorescence and Raman imaging

2021 ◽  
Author(s):  
Cristian Manzoni ◽  
Gianluca Valentini ◽  
Andrea Bassi ◽  
Renzo Vanna ◽  
Giulio Cerullo ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Raman Imaging ◽  
Wide Field

A wide-field high-throughput Hyperspectral Microscope based on an Ultrastable Common-Path Interferometer

2021 ◽  
Author(s):  
B. Ardini ◽  
G. Valentini ◽  
A. Bassi ◽  
A. Candeo ◽  
G. Cerullo ◽  
...  
Keyword(s):  
High Throughput ◽  
Wide Field ◽  
Common Path

scholarly journals Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope

2012 ◽  
Vol 20 (15) ◽  
pp. 16195 ◽  
Author(s):  
Yusuke Oshima ◽  
Hidetoshi Sato ◽  
Hiroko Kajiura-Kobayashi ◽  
Tetsuaki Kimura ◽  
Kiyoshi Naruse ◽  
...  
Keyword(s):  
Light Sheet ◽  
Raman Imaging ◽  
Wide Field ◽  
Optical Sectioning

A practical wide-field Raman imaging method with high spectral and spatial resolution

2018 ◽  
Vol 89 (8) ◽  
pp. 083103 ◽  
Author(s):  
Haibo Li ◽  
Wenhua Luo ◽  
Gan Li ◽  
Guangfeng Zhang ◽  
Pengcheng Zhang ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Raman Imaging ◽  
Imaging Method ◽  
Wide Field

Multiplexed Fourier Transform Infrared and Raman Imaging

2021 ◽  
pp. 299-312
Author(s):  
Guillermo Quintás ◽  
Bayden R. Wood ◽  
Hugh J. Byrne ◽  
David Perez-Guaita
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Raman Imaging

High throughput nutritional profiling of pea seeds using Fourier transform mid-infrared spectroscopy

2020 ◽  
Vol 309 ◽  
pp. 125585 ◽  
Author(s):  
Chithra Karunakaran ◽  
Perumal Vijayan ◽  
Jarvis Stobbs ◽  
Ramandeep Kaur Bamrah ◽  
Gene Arganosa ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
High Throughput ◽  
Mid Infrared ◽  
Mid Infrared Spectroscopy ◽  
Pea Seeds ◽  
Nutritional Profiling

scholarly journals Metabolic Fingerprinting with Fourier-Transform Infrared (FTIR) Spectroscopy: Towards a High-Throughput Screening Assay for Antibiotic Discovery and Mechanism-of-Action Elucidation

Metabolites ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 145
Author(s):  
Bernardo Ribeiro da Cunha ◽  
Luís P. Fonseca ◽  
Cecília R.C. Calado
Keyword(s):  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
High Throughput ◽  
Mechanism Of Action ◽  
High Throughput Screening ◽  
Fourier Transform Infrared ◽  
Metabolic Fingerprinting ◽  
High Throughput Screening Assay ◽  
Metabolic Fingerprint ◽  
Antibiotic Discovery

The discovery of antibiotics has been slowing to a halt. Phenotypic screening is once again at the forefront of antibiotic discovery, yet Mechanism-Of-Action (MOA) identification is still a major bottleneck. As such, methods capable of MOA elucidation coupled with the high-throughput screening of whole cells are required now more than ever, for which Fourier-Transform Infrared (FTIR) spectroscopy is a promising metabolic fingerprinting technique. A high-throughput whole-cell FTIR spectroscopy-based bioassay was developed to reveal the metabolic fingerprint induced by 15 antibiotics on the Escherichia coli metabolism. Cells were briefly exposed to four times the minimum inhibitory concentration and spectra were quickly acquired in the high-throughput mode. After preprocessing optimization, a partial least squares discriminant analysis and principal component analysis were conducted. The metabolic fingerprints obtained with FTIR spectroscopy were sufficiently specific to allow a clear distinction between different antibiotics, across three independent cultures, with either analysis algorithm. These fingerprints were coherent with the known MOA of all the antibiotics tested, which include examples that target the protein, DNA, RNA, and cell wall biosynthesis. Because FTIR spectroscopy acquires a holistic fingerprint of the effect of antibiotics on the cellular metabolism, it holds great potential to be used for high-throughput screening in antibiotic discovery and possibly towards a better understanding of the MOA of current antibiotics.

Wide field of view, ultracompact static Fourier-transform spectrometer

1997 ◽  
Vol 68 (1) ◽  
pp. 30-33 ◽  
Author(s):  
D. Steers ◽  
B. A. Patterson ◽  
W. Sibbett ◽  
M. J. Padgett
Keyword(s):  
Fourier Transform ◽  
Field Of View ◽  
Wide Field ◽  
Fourier Transform Spectrometer ◽  
Wide Field Of View

scholarly journals Rapid, high-throughput, and quantitative determination of orange juice adulteration by Fourier-transform infrared spectroscopy

2016 ◽  
Vol 8 (28) ◽  
pp. 5581-5586 ◽  
Author(s):  
David I. Ellis ◽  
Joanne Ellis ◽  
Howbeer Muhamadali ◽  
Yun Xu ◽  
Andrew B. Horn ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Quantitative Determination ◽  
Fourier Transform Infrared Spectroscopy ◽  
High Throughput ◽  
Orange Juice ◽  
Fourier Transform Infrared ◽  
High Price

Orange juice is a hugely popular, widely consumed, and high price commodity typically traded in a concentrate form making it highly susceptible to adulteration.

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