Multimodal Chemical Nano-imaging and Spectroscopy through Peak Force Infrared Microscopy

2021 ◽  
Author(s):  
Xiaoji G. Xu
Keyword(s):  
Peak Force ◽  
Infrared Microscopy ◽  
Imaging And Spectroscopy ◽  
Nano Imaging

scholarly journals Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Infrared Imaging ◽  
Polymer Surface ◽  
Fluid Phase ◽  
Peak Force ◽  
Label Free ◽  
Imaging And Spectroscopy ◽  
Nano Imaging ◽  
Solid Liquid Interface ◽  
Soft Matters

<a>Peak force infrared (PFIR) microscopy is an emerging atomic force microscopy that bypasses Abbe’s diffraction limit in achieving chemical nano-imaging and spectroscopy. The PFIR microscopy mechanically detects the infrared photothermal responses in the dynamic tip-sample contact of peak force tapping mode, and has been applied for a variety of samples, ranging from soft matters, photovoltaics heterojunctions, to polaritonic materials under the air conditions. In this article, we develop and demonstrate the PFIR microscopy in the liquid phase for soft matters and biological samples. With the capability of controlling fluid compositions on demand, the liquid-phase peak force infrared (LiPFIR) microscopy enables <i>in situ </i>tracking the polymer surface reorganization in fluids and detecting the product of click chemical reaction in the aqueous phase. Both broadband spectroscopy and infrared imaging with ~ 10 nm spatial resolution are benchmarked in the fluid phase, together with complementary mechanical information. We also demonstrate the LiPFIR microscopy on revealing the chemical composition of a budding site of yeast cell wall particles in water as an application on biological structures. The label-free, non-destructive chemical nano-imaging and spectroscopic capabilities of the LiPFIR microscopy will facilitate the investigations of soft matters and their transformations at the solid/liquid interface.</a>

scholarly journals Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Infrared Imaging ◽  
Polymer Surface ◽  
Fluid Phase ◽  
Peak Force ◽  
Label Free ◽  
Imaging And Spectroscopy ◽  
Nano Imaging ◽  
Solid Liquid Interface ◽  
Soft Matters

<a>Peak force infrared (PFIR) microscopy is an emerging atomic force microscopy that bypasses Abbe’s diffraction limit in achieving chemical nano-imaging and spectroscopy. The PFIR microscopy mechanically detects the infrared photothermal responses in the dynamic tip-sample contact of peak force tapping mode, and has been applied for a variety of samples, ranging from soft matters, photovoltaics heterojunctions, to polaritonic materials under the air conditions. In this article, we develop and demonstrate the PFIR microscopy in the liquid phase for soft matters and biological samples. With the capability of controlling fluid compositions on demand, the liquid-phase peak force infrared (LiPFIR) microscopy enables <i>in situ </i>tracking the polymer surface reorganization in fluids and detecting the product of click chemical reaction in the aqueous phase. Both broadband spectroscopy and infrared imaging with ~ 10 nm spatial resolution are benchmarked in the fluid phase, together with complementary mechanical information. We also demonstrate the LiPFIR microscopy on revealing the chemical composition of a budding site of yeast cell wall particles in water as an application on biological structures. The label-free, non-destructive chemical nano-imaging and spectroscopic capabilities of the LiPFIR microscopy will facilitate the investigations of soft matters and their transformations at the solid/liquid interface.</a>

Liquid-Phase Peak Force Infrared Microscopy

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Yan Yu ◽  
Xiaoji Xu
Keyword(s):  
Liquid Phase ◽  
Spatial Resolution ◽  
Shear Force ◽  
Polymer Surface ◽  
Surface Damage ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Contact Mode ◽  
Force Microscopy ◽  
Hydrogen Deuterium

Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

Dual-Color Peak Force Infrared Microscopy

2021 ◽  
Author(s):  
Qing Xie ◽  
Jared Wiemann ◽  
Yan Yu ◽  
Xiaoji G. Xu
Keyword(s):  
Peak Force ◽  
Infrared Microscopy ◽  
Dual Color

scholarly journals Liquid-Phase Peak Force Infrared Microscopy

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Spatial Resolution ◽  
Shear Force ◽  
Polymer Surface ◽  
Surface Damage ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Contact Mode ◽  
Force Microscopy ◽  
Hydrogen Deuterium

Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

Nanoscale spectroscopic and mechanical characterization of individual aerosol particles using peak force infrared microscopy

2017 ◽  
Vol 53 (53) ◽  
pp. 7397-7400 ◽  
Author(s):  
Le Wang ◽  
Dandan Huang ◽  
Chak K. Chan ◽  
Yong Jie Li ◽  
Xiaoji G. Xu
Keyword(s):  
Particulate Matter ◽  
Spatial Resolution ◽  
Infrared Imaging ◽  
Mechanical Characterization ◽  
Aerosol Particles ◽  
Fine Particulate Matter ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Fine Particulate

Individual fine particulate matter (PM2.5) particles are revealed with mechanical mapping and infrared imaging at 10 nm spatial resolution.

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