Peak force infrared microscopy for label-free chemical imaging at sub 10 nm spatial resolution

2020 ◽  
Author(s):  
Le Wang ◽  
Guanghong Zeng ◽  
Gilbert C. Walker ◽  
Xiaoji Xu
Keyword(s):  
Spatial Resolution ◽  
Chemical Imaging ◽  
Peak Force ◽  
Label Free ◽  
Infrared Microscopy

scholarly journals Depth-resolved mid-infrared photothermal imaging of living cells and organisms with submicrometer spatial resolution

2016 ◽  
Vol 2 (9) ◽  
pp. e1600521 ◽  
Author(s):  
Delong Zhang ◽  
Chen Li ◽  
Chi Zhang ◽  
Mikhail N. Slipchenko ◽  
Gregory Eakins ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Single Cells ◽  
Chemical Imaging ◽  
Detection Sensitivity ◽  
Living Systems ◽  
Live Cells ◽  
Photothermal Effect ◽  
Label Free ◽  
Infrared Microscopy ◽  
Mid Infrared

Chemical contrast has long been sought for label-free visualization of biomolecules and materials in complex living systems. Although infrared spectroscopic imaging has come a long way in this direction, it is thus far only applicable to dried tissues because of the strong infrared absorption by water. It also suffers from low spatial resolution due to long wavelengths and lacks optical sectioning capabilities. We overcome these limitations through sensing vibrational absorption–induced photothermal effect by a visible laser beam. Our mid-infrared photothermal (MIP) approach reached 10 μM detection sensitivity and submicrometer lateral spatial resolution. This performance has exceeded the diffraction limit of infrared microscopy and allowed label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells were visualized. We further demonstrated in vivo MIP imaging of lipids and proteins inCaenorhabditis elegans. The reported MIP imaging technology promises broad applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

Liquid-Phase Peak Force Infrared Microscopy

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Yan Yu ◽  
Xiaoji Xu
Keyword(s):  
Liquid Phase ◽  
Spatial Resolution ◽  
Shear Force ◽  
Polymer Surface ◽  
Surface Damage ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Contact Mode ◽  
Force Microscopy ◽  
Hydrogen Deuterium

Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

scholarly journals Liquid-Phase Peak Force Infrared Microscopy

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Spatial Resolution ◽  
Shear Force ◽  
Polymer Surface ◽  
Surface Damage ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Contact Mode ◽  
Force Microscopy ◽  
Hydrogen Deuterium

Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

scholarly journals Inside Cover: Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy (Angew. Chem. Int. Ed. 47/2009)

2009 ◽  
Vol 48 (47) ◽  
pp. 8798-8798
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy

2009 ◽  
Vol 121 (47) ◽  
pp. 9152-9156 ◽  
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Innentitelbild: Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy (Angew. Chem. 47/2009)

2009 ◽  
Vol 121 (47) ◽  
pp. 8956-8956
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Nanoscale spectroscopic and mechanical characterization of individual aerosol particles using peak force infrared microscopy

2017 ◽  
Vol 53 (53) ◽  
pp. 7397-7400 ◽  
Author(s):  
Le Wang ◽  
Dandan Huang ◽  
Chak K. Chan ◽  
Yong Jie Li ◽  
Xiaoji G. Xu
Keyword(s):  
Particulate Matter ◽  
Spatial Resolution ◽  
Infrared Imaging ◽  
Mechanical Characterization ◽  
Aerosol Particles ◽  
Fine Particulate Matter ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Fine Particulate

Individual fine particulate matter (PM2.5) particles are revealed with mechanical mapping and infrared imaging at 10 nm spatial resolution.

Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy

2009 ◽  
Vol 48 (47) ◽  
pp. 8990-8994 ◽  
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Generalized Heterodyne Configurations for Photo-induced Force Microscopy

2019 ◽  
Author(s):  
Le Wang ◽  
Devon Jakob ◽  
Haomin Wang ◽  
Alexis Apostolos ◽  
Marcos M. Pires ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Repetition Rate ◽  
Modulation Frequency ◽  
High Harmonic ◽  
Chemical Imaging ◽  
Heterodyne Detection ◽  
Force Microscopy ◽  
Wide Range ◽  
The Difference ◽  
Afm Cantilever

<div>Infrared chemical microscopy through mechanical probing of light-matter interactions by atomic force microscopy (AFM) bypasses the diffraction limit. One increasingly popular technique is photo-induced force microscopy (PiFM), which utilizes the mechanical heterodyne signal detection between cantilever mechanical resonant oscillations and the photo induced force from light-matter interaction. So far, photo induced force microscopy has been operated in only one heterodyne configuration. In this article, we generalize heterodyne configurations of photoinduced force microscopy by introducing two new schemes: harmonic heterodyne detection and sequential heterodyne detection. In harmonic heterodyne detection, the laser repetition rate matches integer fractions of the difference between the two mechanical resonant modes of the AFM cantilever. The high harmonic of the beating from the photothermal expansion mixes with the AFM cantilever oscillation to provide PiFM signal. In sequential heterodyne detection, the combination of the repetition rate of laser pulses and polarization modulation frequency matches the difference between two AFM mechanical modes, leading to detectable PiFM signals. These two generalized heterodyne configurations for photo induced force microscopy deliver new avenues for chemical imaging and broadband spectroscopy at ~10 nm spatial resolution. They are suitable for a wide range of heterogeneous materials across various disciplines: from structured polymer film, polaritonic boron nitride materials, to isolated bacterial peptidoglycan cell walls. The generalized heterodyne configurations introduce flexibility for the implementation of PiFM and related tapping mode AFM-IR, and provide possibilities for additional modulation channel in PiFM for targeted signal extraction with nanoscale spatial resolution.</div>

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