Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging

2021 ◽  
Author(s):  
Jakub Nedbal ◽  
Francesco Mattioli Della Rocca ◽  
Richard Walker ◽  
Robert K. Henderson ◽  
Klaus Suhling
Keyword(s):  
Single Photon ◽  
Biological Imaging ◽  
Time Resolved ◽  
Array Measurements

Single Point PICA Probing with an Avalanche Photo-Diode

2001 ◽  
Author(s):  
Mike Bruce ◽  
Rama R. Goruganthu ◽  
Shawn McBride ◽  
David Bethke ◽  
J.M. Chin
Keyword(s):  
Single Photon ◽  
Single Point ◽  
Photon Counting ◽  
Ring Oscillator ◽  
Time Resolved ◽  
Single Photon Counting ◽  
Photo Diode ◽  
Avalanche Photo Diode ◽  
Photo Multiplier Tube ◽  
Channel Plate

Abstract For time resolved hot carrier emission from the backside, an alternate approach is demonstrated termed single point PICA. The single point approach records time resolved emission from an individual transistor using time-correlated-single-photon counting and an avalanche photo-diode. The avalanche photo-diode has a much higher quantum efficiency than micro-channel plate photo-multiplier tube based imaging cameras typically used in earlier approaches. The basic system is described and demonstrated from the backside on a ring oscillator circuit.

scholarly journals Super-resolution time-resolved imaging using computational sensor fusion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
C. Callenberg ◽  
A. Lyons ◽  
D. den Brok ◽  
A. Fatima ◽  
A. Turpin ◽  
...  
Keyword(s):  
Sensor Fusion ◽  
Spatial Resolution ◽  
Temporal Resolution ◽  
Single Photon ◽  
Super Resolution ◽  
Numerical Data ◽  
Data Cube ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Temporal Precision

AbstractImaging across both the full transverse spatial and temporal dimensions of a scene with high precision in all three coordinates is key to applications ranging from LIDAR to fluorescence lifetime imaging. However, compromises that sacrifice, for example, spatial resolution at the expense of temporal resolution are often required, in particular when the full 3-dimensional data cube is required in short acquisition times. We introduce a sensor fusion approach that combines data having low-spatial resolution but high temporal precision gathered with a single-photon-avalanche-diode (SPAD) array with data that has high spatial but no temporal resolution, such as that acquired with a standard CMOS camera. Our method, based on blurring the image on the SPAD array and computational sensor fusion, reconstructs time-resolved images at significantly higher spatial resolution than the SPAD input, upsampling numerical data by a factor $$12 \times 12$$ 12 × 12 , and demonstrating up to $$4 \times 4$$ 4 × 4 upsampling of experimental data. We demonstrate the technique for both LIDAR applications and FLIM of fluorescent cancer cells. This technique paves the way to high spatial resolution SPAD imaging or, equivalently, FLIM imaging with conventional microscopes at frame rates accelerated by more than an order of magnitude.

scholarly journals A Versatile Setup for Time-Resolved Functional Near Infrared Spectroscopy Based on Fast-Gated Single-Photon Avalanche Diode and on Four-Wave Mixing Laser

2019 ◽  
Vol 9 (11) ◽  
pp. 2366 ◽  
Author(s):  
Laura Di Sieno ◽  
Alberto Dalla Mora ◽  
Alessandro Torricelli ◽  
Lorenzo Spinelli ◽  
Rebecca Re ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Single Photon ◽  
Wave Mixing ◽  
Functional Near Infrared Spectroscopy ◽  
Time Resolved ◽  
Diffusive Medium ◽  
Spectroscopy System

In this paper, a time-domain fast gated near-infrared spectroscopy system is presented. The system is composed of a fiber-based laser providing two pulsed sources and two fast gated detectors. The system is characterized on phantoms and was tested in vivo, showing how the gating approach can improve the contrast and contrast-to-noise-ratio for detection of absorption perturbation inside a diffusive medium, regardless of source-detector separation.

scholarly journals The time-resolved hard X-ray diffraction endstation KMC-3 XPP at BESSY II

2021 ◽  
Vol 28 (3) ◽  
Author(s):  
Matthias Rössle ◽  
Wolfram Leitenberger ◽  
Matthias Reinhardt ◽  
Azize Koç ◽  
Jan Pudell ◽  
...  
Keyword(s):  
Storage Ring ◽  
Single Photon ◽  
Laser System ◽  
High Vacuum ◽  
Laser Pulses ◽  
Pixel Detector ◽  
X Ray Diffraction ◽  
Optical Pump ◽  
Time Resolved ◽  
X Ray

The time-resolved hard X-ray diffraction endstation KMC-3 XPP for optical pump/X-ray probe experiments at the electron storage ring BESSY II is dedicated to investigating the structural response of thin film samples and heterostructures after their excitation with ultrashort laser pulses and/or electric field pulses. It enables experiments with access to symmetric and asymmetric Bragg reflections via a four-circle diffractometer and it is possible to keep the sample in high vacuum and vary the sample temperature between ∼15 K and 350 K. The femtosecond laser system permanently installed at the beamline allows for optical excitation of the sample at 1028 nm. A non-linear optical setup enables the sample excitation also at 514 nm and 343 nm. A time-resolution of 17 ps is achieved with the `low-α' operation mode of the storage ring and an electronic variation of the delay between optical pump and hard X-ray probe pulse conveniently accesses picosecond to microsecond timescales. Direct time-resolved detection of the diffracted hard X-ray synchrotron pulses use a gated area pixel detector or a fast point detector in single photon counting mode. The range of experiments that are reliably conducted at the endstation and that detect structural dynamics of samples excited by laser pulses or electric fields are presented.

scholarly journals High-time-resolved 64-channel single-flux quantum-based address encoder integrated with a multi-pixel superconducting nanowire single-photon detector

2018 ◽  
Vol 26 (22) ◽  
pp. 29045 ◽  
Author(s):  
Shigeyuki Miyajima ◽  
Masahiro Yabuno ◽  
Shigehito Miki ◽  
Taro Yamashita ◽  
Hirotaka Terai
Keyword(s):  
Single Photon ◽  
High Time ◽  
Flux Quantum ◽  
Photon Detector ◽  
Single Photon Detector ◽  
Time Resolved ◽  
Superconducting Nanowire

Compact, Low-Power and Fully Reconfigurable 10 ps Resolution, 160 Range, Time-Resolved Single-Photon Counting System

2016 ◽  
Vol 16 (10) ◽  
pp. 3827-3833 ◽  
Author(s):  
Davide Tamborini ◽  
Mauro Buttafava ◽  
Alessandro Ruggeri ◽  
Franco Zappa
Keyword(s):  
Low Power ◽  
Single Photon ◽  
Photon Counting ◽  
Counting System ◽  
Time Resolved ◽  
Single Photon Counting

scholarly journals Gated SPAD Arrays for Single-Photon Time-Resolved Imaging and Spectroscopy

2019 ◽  
Vol 11 (6) ◽  
pp. 1-10 ◽  
Author(s):  
Enrico Conca ◽  
Iris Cusini ◽  
Fabio Severini ◽  
Rudi Lussana ◽  
Franco Zappa ◽  
...  
Keyword(s):  
Single Photon ◽  
Time Resolved ◽  
Imaging And Spectroscopy ◽  
Time Resolved Imaging

scholarly journals Investigations of the Energy Transfer in the Phycobilisome Antenna of Arthrospira platensis Using Femtosecond Spectroscopy

2020 ◽  
Vol 10 (11) ◽  
pp. 4045
Author(s):  
Alexandra Fălămaș ◽  
Sebastian A. Porav ◽  
Valer Tosa
Keyword(s):  
Energy Transfer ◽  
Transient Absorption ◽  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Excitation Energy Transfer ◽  
Arthrospira Platensis ◽  
Absorption Data ◽  
Time Resolved ◽  
Photocurrent Amplification

Understanding the energy transfer in phycobilisomes extracted from cyanobacteria can be used for building biomimetic hybrid systems for optimized solar energy collection and photocurrent amplification. In this paper, we applied time-resolved absorption and fluorescence spectroscopy to investigate the ultrafast dynamics in a hemidiscoidal phycobilisome obtained from Arthrospira platensis. We obtained the steady-state and time-resolved optical properties and identified the possible pathways of the excitation energy transfer in the phycobilisome and its components, phycocyanin and allophycocyanin. The transient absorption data were studied using global analysis and revealed the existence of ultrafast kinetics down to 850 fs in the phycobilisome. The fluorescence lifetimes in the nanosecond time-scale assigned to the final emitters in each sample were obtained from the time-correlated single photon counting fluorescence experiments.

scholarly journals Ultrafast F2− photodissociation by intense laser pulses: a time-resolved fragment imaging study

2019 ◽  
Vol 205 ◽  
pp. 09012
Author(s):  
Abhishek Shahi ◽  
Yishai Albeck ◽  
Daniel Strasser
Keyword(s):  
Single Photon ◽  
Imaging Study ◽  
Laser Pulses ◽  
Intense Laser ◽  
Time Resolved ◽  
Intense Field ◽  
Intense Laser Pulses ◽  
Ultrafast Pulses ◽  
Coincidence Imaging ◽  
Field Dissociation

We present time-resolved coincidence imaging of F2− photodissociation by 400nm and intense 800nm ultrafast pulses. Coincidence fragment imaging reveals parallel and perpendicular single photon dissociation on 2Σg+ and 2πg states, and additional intense-field dissociation features.

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