Comprehensive evaluation of ex vivo and in vivo tissues with ultraviolet photoacoustic remote sensing virtual histology and spectral-domain optical coherence tomography

2021 ◽  
Author(s):  
Matthew T. Martell ◽  
Nathaniel J. M. Haven ◽  
Brendon S. Restall ◽  
Pradyumna Kedarisetti ◽  
Roger J. Zemp
Keyword(s):  
Remote Sensing ◽  
Optical Coherence Tomography ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Virtual Histology

scholarly journals Design and Implementation Guidelines for a Modular Spectral-Domain Optical Coherence Tomography Scanner

2018 ◽  
Vol 2018 ◽  
pp. 1-22 ◽  
Author(s):  
Farid Atry ◽  
Israel Jacob De La Rosa ◽  
Kevin R. Rarick ◽  
Ramin Pashaie
Keyword(s):  
Optical Coherence Tomography ◽  
Imaging System ◽  
Optical Design ◽  
Spectral Domain ◽  
Design Parameters ◽  
Optical Coherence ◽  
Custom Made ◽  
Essential Knowledge ◽  
Sd Oct

In the past decades, spectral-domain optical coherence tomography (SD-OCT) has transformed into a widely popular imaging technology which is used in many research and clinical applications. Despite such fast growth in the field, the technology has not been readily accessible to many research laboratories either due to the cost or inflexibility of the commercially available systems or due to the lack of essential knowledge in the field of optics to develop custom-made scanners that suit specific applications. This paper aims to provide a detailed discussion on the design and development process of a typical SD-OCT scanner. The effects of multiple design parameters, for the main optical and optomechanical components, on the overall performance of the imaging system are analyzed and discussions are provided to serve as a guideline for the development of a custom SD-OCT system. While this article can be generalized for different applications, we will demonstrate the design of a SD-OCT system and representative results for in vivo brain imaging. We explain procedures to measure the axial and transversal resolutions and field of view of the system and to understand the discrepancies between the experimental and theoretical values. The specific aim of this piece is to facilitate the process of constructing custom-made SD-OCT scanners for research groups with minimum understanding of concepts in optical design and medical imaging.

In vivo imaging with high-speed full-range complex spectral domain optical coherence tomography

2005 ◽  
Author(s):  
Erich Goetzinger ◽  
Michael Pircher ◽  
Rainer A. Leitgeb ◽  
Adolf F. Fercher ◽  
Christoph K. Hitzenberger
Keyword(s):  
Optical Coherence Tomography ◽  
In Vivo Imaging ◽  
High Speed ◽  
Full Range ◽  
Spectral Domain ◽  
Optical Coherence

In Vivo Assessment of Rodent Retinal Structure Using Spectral Domain Optical Coherence Tomography

2011 ◽  
pp. 489-494 ◽  
Author(s):  
M. Dominik Fischer ◽  
Gesine Huber ◽  
Francois Paquet-Durand ◽  
Peter Humphries ◽  
T. Michael Redmond ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Retinal Structure ◽  
In Vivo Assessment

scholarly journals Polarization sensitive optical coherence tomography with single input for imaging depth-resolved collagen organizations

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Peijun Tang ◽  
Mitchell A. Kirby ◽  
Nhan Le ◽  
Yuandong Li ◽  
Nicole Zeinstra ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Polarization State ◽  
Transmission Model ◽  
Optical Coherence ◽  
Healthy Human ◽  
Collagen Organization ◽  
Single Input

AbstractCollagen organization plays an important role in maintaining structural integrity and determining tissue function. Polarization-sensitive optical coherence tomography (PSOCT) is a promising noninvasive three-dimensional imaging tool for mapping collagen organization in vivo. While PSOCT systems with multiple polarization inputs have demonstrated the ability to visualize depth-resolved collagen organization, systems, which use a single input polarization state have not yet demonstrated sufficient reconstruction quality. Herein we describe a PSOCT based polarization state transmission model that reveals the depth-dependent polarization state evolution of light backscattered within a birefringent sample. Based on this model, we propose a polarization state tracing method that relies on a discrete differential geometric analysis of the evolution of the polarization state in depth along the Poincare sphere for depth-resolved birefringent imaging using only one single input polarization state. We demonstrate the ability of this method to visualize depth-resolved myocardial architecture in both healthy and infarcted rodent hearts (ex vivo) and collagen structures responsible for skin tension lines at various anatomical locations on the face of a healthy human volunteer (in vivo).

scholarly journals Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents

2020 ◽  
Vol 245 (18) ◽  
pp. 1629-1636
Author(s):  
Ruiming Kong ◽  
Wenjuan Wu ◽  
Rui Qiu ◽  
Lei Gao ◽  
Fengxian Du ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Light Attenuation ◽  
Anterior Segment ◽  
Posterior Segment ◽  
Glycerol Solution ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Imaging Depth

Optical coherence tomography has become an indispensable diagnostic tool in ophthalmology for imaging the retina and the anterior segment of the eye. However, the imaging depth of optical coherence tomography is limited by light attenuation in tissues due to optical scattering and absorption. In this study of rabbit eye both ex vivo and in vivo, optical coherence tomography imaging depth of the anterior and posterior segments of the eye was extended by using optical clearing agents to reduce multiple scattering. The sclera, the iris, and the ciliary body were clearly visualized by direct application of glycerol at an incision on the conjunctiva, and the posterior boundary of sclera and even the deeper tissues were detected by submerging the posterior segment of eye in glycerol solution ex vivo or by retro-bulbar injection of glycerol in vivo. The ex vivo rabbit eyes recovered to their original state in 60 s after saline-wash treatment, and normal optical coherence tomography images of the posterior segment of the sample eyes proved the self-recovery of in vivo performance. Signal intensities of optical coherence tomography images obtained before and after glycerol treatment were compared to analysis of the effect of optical clearing. To the best of our knowledge, this is the first study for imaging depth extension of optical coherence tomography in both the anterior and posterior segments of eye by using optical clearing agents.

scholarly journals In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve

2004 ◽  
Vol 12 (3) ◽  
pp. 367 ◽  
Author(s):  
N. A. Nassif ◽  
B. Cense ◽  
B. H. Park ◽  
M. C. Pierce ◽  
S. H. Yun ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optic Nerve ◽  
High Resolution ◽  
Spectral Domain ◽  
Human Retina ◽  
Optical Coherence
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