Label-free measurement of cell mechanical properties with digital holographic microscopy

2021 ◽  
Author(s):  
Erik Bélanger ◽  
Sara Mattar ◽  
Sébastien Boutet ◽  
Gabrielle Jess ◽  
Isabelle St-Amour ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Cell Mechanical Properties ◽  
Holographic Microscopy

scholarly journals Label-free fingerprinting of tumor cells in bulk flow using inline digital holographic microscopy

2017 ◽  
Vol 8 (2) ◽  
pp. 536 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Bulk Flow ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy

Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

2010 ◽  
Vol 35 (24) ◽  
pp. 4102 ◽  
Author(s):  
Etienne Shaffer ◽  
Corinne Moratal ◽  
Pierre Magistretti ◽  
Pierre Marquet ◽  
Christian Depeursinge
Keyword(s):  
Second Harmonic ◽  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Label Free ◽  
Biological Specimen ◽  
Harmonic Phase ◽  
Holographic Microscopy ◽  
Harmonic Phase Imaging

scholarly journals Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy

2010 ◽  
Vol 15 (3) ◽  
pp. 036009 ◽  
Author(s):  
Björn Kemper ◽  
Andreas Bauwens ◽  
Angelika Vollmer ◽  
Steffi Ketelhut ◽  
Patrik Langehanenberg ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Division ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy

Digital holographic microscopy for quantitative and label-free oral cytology evaluation

2020 ◽  
Vol 59 (02) ◽  
pp. 1 ◽  
Author(s):  
Hanu Phani Ram Gurram ◽  
Prashanth Panta ◽  
Vimal Prabhu Pandiyan ◽  
Inayathullah Ghori ◽  
Renu John
Keyword(s):  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy ◽  
Oral Cytology

scholarly journals Whole-Cell Multiparameter Assay for Ricin and Abrin Activity-Based Digital Holographic Microscopy

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 174 ◽  
Author(s):  
Efi Makdasi ◽  
Orly Laskar ◽  
Elad Milrot ◽  
Ofir Schuster ◽  
Shlomo Shmaya ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Morphological Changes ◽  
Annexin V ◽  
Time Lapse ◽  
Digital Holographic Microscopy ◽  
Optical Parameters ◽  
Label Free ◽  
Calculated Volume ◽  
Apoptosis Assay ◽  
Holographic Microscopy

Ricin and abrin are ribosome-inactivating proteins leading to inhibition of protein synthesis and cell death. These toxins are considered some of the most potent and lethal toxins against which there is no available antidote. Digital holographic microscopy (DHM) is a time-lapse, label-free, and noninvasive imaging technique that can provide phase information on morphological features of cells. In this study, we employed DHM to evaluate the morphological changes of cell lines during ricin and abrin intoxication. We showed that the effect of these toxins is characterized by a decrease in cell confluence and changes in morphological parameters such as cell area, perimeter, irregularity, and roughness. In addition, changes in optical parameters such as phase-shift, optical thickness, and effective-calculated volume were observed. These effects were completely inhibited by specific neutralizing antibodies. An enhanced intoxication effect was observed for preadherent compared to adherent cells, as was detected in early morphology changes and confirmed by annexin V/propidium iodide (PI) apoptosis assay. Detection of the dynamic changes in cell morphology at initial stages of cell intoxication by DHM emphasizes the highly sensitive and rapid nature of this method, allowing the early detection of active toxins.

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