Label-free observation of three-dimensional morphology change of a single PC12 cell by digital holographic microscopy

2012 ◽  
Vol 429 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Tanveer Ahamd Mir ◽  
Hiroaki Shinohara
Keyword(s):  
Pc12 Cell ◽  
Three Dimensional ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Morphology Change ◽  
Holographic Microscopy

scholarly journals Subpopulations of Organoid-Forming Cells Have Different Motility

2020 ◽  
Vol 10 (13) ◽  
pp. 4673
Author(s):  
David Gomez Jimenez ◽  
Sofia Carreira Santos ◽  
Lennart Greiff ◽  
Kersti Alm ◽  
Malin Lindstedt
Keyword(s):  
Tumor Cells ◽  
Three Dimensional ◽  
Cell Aggregation ◽  
Digital Holographic Microscopy ◽  
Optical Parameters ◽  
Label Free ◽  
Light Passing ◽  
Tumorigenic Potential ◽  
Holographic Microscopy ◽  
Tumor Organoids

Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 105 µm3. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44+ cells displayed greater motility and tumor-forming capacity than those of CD44− cells. These results were in line with previous reports highlighting increased invasive and tumorigenic potential in tumor cells expressing high levels of CD44. Our method provides insight into the formation of tumor organoids, and could be used to assess stemness-associated biomarkers and drug screenings on the basis of tumor organoids.

scholarly journals 3D monitoring of the surface slippage effect on micro-particle sedimentation by digital holographic microscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Majid Panahi ◽  
Ramin Jamali ◽  
Vahideh Farzam Rad ◽  
Mojtaba Khorasani ◽  
Ahamd Darudi ◽  
...  
Keyword(s):  
Slip Length ◽  
Particle Interaction ◽  
Three Dimensional ◽  
Digital Holographic Microscopy ◽  
Particle Sedimentation ◽  
Micro Particles ◽  
Slippage Effect ◽  
Holographic Microscopy ◽  
3D Information ◽  
Experimental Findings

AbstractIn several phenomena in biology and industry, it is required to understand the comprehensive behavior of sedimenting micro-particles in fluids. Here, we use the numerical refocusing feature of digital holographic microscopy (DHM) to investigate the slippage effect on micro-particle sedimentation near a flat wall. DHM provides quantitative phase contrast and three-dimensional (3D) imaging in arbitrary time scales, which suggests it as an elegant approach to investigate various phenomena, including dynamic behavior of colloids. 3D information is obtained by post-processing of the recorded digital holograms. Through analysis of 3D trajectories and velocities of multiple sedimenting micro-particles, we show that proximity to flat walls of higher slip lengths causes faster sedimentation. The effect depends on the ratio of the particle size to (1) the slip length and (2) its distance to the wall. We corroborate our experimental findings by a theoretical model which considers both the proximity and the particle interaction to a wall of different hydrophobicity in the hydrodynamic forces.

scholarly journals Label-free fingerprinting of tumor cells in bulk flow using inline digital holographic microscopy

2017 ◽  
Vol 8 (2) ◽  
pp. 536 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Bulk Flow ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy

Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

2010 ◽  
Vol 35 (24) ◽  
pp. 4102 ◽  
Author(s):  
Etienne Shaffer ◽  
Corinne Moratal ◽  
Pierre Magistretti ◽  
Pierre Marquet ◽  
Christian Depeursinge
Keyword(s):  
Second Harmonic ◽  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Label Free ◽  
Biological Specimen ◽  
Harmonic Phase ◽  
Holographic Microscopy ◽  
Harmonic Phase Imaging

Three Dimensional Imaging Of Cells Through Digital Holographic Microscopy

1984 ◽  
Author(s):  
F. Beltrame ◽  
B. Bianco ◽  
A. Geraci ◽  
G. Laub ◽  
P. Schwarzman
Keyword(s):  
Three Dimensional ◽  
Digital Holographic Microscopy ◽  
Three Dimensional Imaging ◽  
Holographic Microscopy
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