Integrated simultaneous multimodality photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy for rabbit ocular imaging in vivo and safety evaluation

2021 ◽  
Author(s):  
Wei Zhang ◽  
Yanxiu Li ◽  
Van Phuc Nguyen ◽  
Yannis M. Paulus ◽  
Xueding Wang
Keyword(s):  
Optical Coherence Tomography ◽  
Fluorescence Microscopy ◽  
Safety Evaluation ◽  
Optical Coherence ◽  
Photoacoustic Microscopy ◽  
Ocular Imaging

scholarly journals Integrating photoacoustic microscopy, optical coherence tomography, OCT angiography, and fluorescence microscopy for multimodal imaging

2020 ◽  
Vol 245 (4) ◽  
pp. 342-347 ◽  
Author(s):  
Arash Dadkhah ◽  
Shuliang Jiao
Keyword(s):  
Optical Coherence Tomography ◽  
Fluorescence Microscopy ◽  
Multimodal Imaging ◽  
Imaging System ◽  
Optical Resolution ◽  
Biological Tissues ◽  
Large Field ◽  
Optical Coherence ◽  
Photoacoustic Microscopy

We have developed a multimodal imaging system, which integrated optical resolution photoacoustic microscopy, optical coherence tomography, optical coherence tomography angiography, and confocal fluorescence microscopy in one platform. The system is able to image complementary features of a biological sample by combining different contrast mechanisms. We achieved fast imaging and large field of view by combining optical scanning with mechanical scanning, similar to our previous publication. We have demonstrated the capability of the multimodal imaging system by imaging a mouse ear in vivo. Impact statement Photoacoustic microscopy-based multimodal imaging technology can provide high-resolution complementary information for biological tissues in vivo. It will potentially bring significant impact on the research and diagnosis of diseases by providing combined structural and functional information.

scholarly journals In Vivo 3D Imaging of Retinal Neovascularization Using Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Imaging

2018 ◽  
Vol 4 (12) ◽  
pp. 150 ◽  
Author(s):  
Van Nguyen ◽  
Yanxiu Li ◽  
Michael Aaberg ◽  
Wei Zhang ◽  
Xueding Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Multimodal Imaging ◽  
Imaging System ◽  
Pathological Process ◽  
Retinal Neovascularization ◽  
Optical Coherence ◽  
Photoacoustic Microscopy ◽  
Ocular Imaging ◽  
Sd Oct

The pathological process of neovascularization of the retina plays a critical role in causing vision loss in several diseases, including diabetes, retinal vein occlusion, and sickle cell disease. Retinal neovascularization can lead to vitreous hemorrhage and retinal detachment, yet the pathological process of neovascularization is a complex phenomenon under active investigation. Understanding and monitoring retinal neovascularization is critically important in clinical ophthalmology. This study describes a novel multimodal ocular imaging system which combines photoacoustic microscopy (PAM) and a spectral domain optical coherence tomography (SD-OCT) to improve the visualization of retinal neovascularization (RNV), their depth, and the surrounding anatomy in living rabbits. RNV was induced in New Zealand rabbits by intravitreal injection of vascular endothelial growth factor (VEGF). The retinal vasculature before and after injection at various times was monitored and evaluated using multimodal imaging including color fundus photography, fluorescein angiography (FA), OCT, and PAM. In vivo experiments demonstrate that PAM imaging distinctly characterized the location as well as the morphology of individual RNV with high contrast at a safe laser energy of 80 nJ. SD-OCT was used to identify a cross-sectional structure of RNV. In addition, dynamic changes in the retinal morphology and retinal neovascularization were observed at day 4, 5, 6, 7, 9, 11, 14, 28, and day 35 after VEGF injection. PAM demonstrated high-resolution optical absorption of hemoglobin and vascular imaging of the retina and choroid with increased depth of penetration. With the current multimodal imaging system, RNV can be easily visualized in both 2D and 3D angiography. This multimodal ocular imaging system provides improved characterization of the microvasculature in a safe manner in larger rabbit eyes.

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