The suppression of the side lobes of the fluorescence light-sheet microscopy with scanned Bessel beam by using the leaky filter

2020 ◽  
Author(s):  
Ding Zijun ◽  
Hu Luohong ◽  
Yuan Liusong ◽  
Yuan Deyi ◽  
Li Xianhong ◽  
...  
Keyword(s):  
Bessel Beam ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Fluorescence Light

scholarly journals Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

OSA Continuum ◽  
2020 ◽  
Vol 3 (8) ◽  
pp. 2195
Author(s):  
Christian Jaques ◽  
Alexander Ernst ◽  
Nadia Mercader ◽  
Michael Liebling
Keyword(s):  
Temporal Resolution ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Fluorescence Light

High-fidelity functional and structural whole-brain imaging with Bessel-beam light-sheet microscopy (Conference Presentation)

2017 ◽  
Author(s):  
Marie Caroline Müllenbroich ◽  
Ludovico Silvestri ◽  
Lapo Turrini ◽  
Antonino Paolo Di Giovanna ◽  
Tommaso Alterini ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Bessel Beam ◽  
Light Sheet ◽  
High Fidelity ◽  
Whole Brain ◽  
Light Sheet Microscopy

scholarly journals Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sota Takanezawa ◽  
Takashi Saitou ◽  
Takeshi Imamura
Keyword(s):  
High Speed ◽  
Bessel Beam ◽  
Light Sheet ◽  
Time Lapse ◽  
Whole Body ◽  
Wide Field ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Two Photon Excitation

AbstractTwo-photon excitation can lower phototoxicity and improve penetration depth, but its narrow excitation range restricts its applications in light-sheet microscopy. Here, we propose simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams. This unit can stretch the beam full width at half maximum of 600–1000 μm with less than a 4-μm waist when using a 10× illumination lens. A two-photon excitation digital scanned light-sheet microscope possessing this range of field of view and ~2–3-μm axial resolution is constructed and used to analyze the cellular dynamics over the whole body of medaka fish. We demonstrate long-term time-lapse observations over several days and high-speed recording with ~3 mm3 volume per 4 s of the embryos. Our system is minimal and suppresses laser power loss, which can broaden applications of two-photon excitation in light-sheet microscopy.

Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam

2018 ◽  
Vol 12 (1) ◽  
pp. e201800094 ◽  
Author(s):  
Hao Jia ◽  
Xianghua Yu ◽  
Yanlong Yang ◽  
Xing Zhou ◽  
Shaohui Yan ◽  
...  
Keyword(s):  
Bessel Beam ◽  
Resolution Enhancement ◽  
Light Sheet ◽  
Double Scanning ◽  
Axial Resolution ◽  
Light Sheet Microscopy

scholarly journals Corrigendum: Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy

2019 ◽  
Vol 13 ◽  
Author(s):  
M. Caroline Müllenbroich ◽  
Lapo Turrini ◽  
Ludovico Silvestri ◽  
Tommaso Alterini ◽  
Ali Gheisari ◽  
...  
Keyword(s):  
Bessel Beam ◽  
Light Sheet ◽  
Systematic Errors ◽  
Functional Studies ◽  
Light Sheet Microscopy

scholarly journals Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy

2018 ◽  
Vol 12 ◽  
Author(s):  
M. Caroline Müllenbroich ◽  
Lapo Turrini ◽  
Ludovico Silvestri ◽  
Tommaso Alterini ◽  
Ali Gheisari ◽  
...  
Keyword(s):  
Bessel Beam ◽  
Light Sheet ◽  
Systematic Errors ◽  
Functional Studies ◽  
Light Sheet Microscopy

scholarly journals Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Giuseppe Di Domenico ◽  
Giancarlo Ruocco ◽  
Cristina Colosi ◽  
Eugenio DelRe ◽  
Giuseppe Antonacci
Keyword(s):  
Bessel Beam ◽  
Light Sheet ◽  
High Contrast ◽  
Light Sheet Microscopy

High-fidelity imaging with Bessel-beam light-sheet microscopy for whole-brain structural and functional studies

2017 ◽  
Author(s):  
M. Caroline Müllenbroich ◽  
Ludovico Silvestri ◽  
Lapo Turrini ◽  
Antonino P. Di Giovanna ◽  
Tommaso Alterini ◽  
...  
Keyword(s):  
Bessel Beam ◽  
Light Sheet ◽  
High Fidelity ◽  
Whole Brain ◽  
Functional Studies ◽  
Light Sheet Microscopy
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