Functional imaging of zebrafish neuronal activity by Bessel beam light-sheet microscopy

2016 ◽  
Author(s):  
L. Turrini ◽  
T. Alterini ◽  
C. Müllenbroich ◽  
A. Gheisari ◽  
L. Sacconi ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Functional Imaging ◽  
Bessel Beam ◽  
Light Sheet ◽  
Light Sheet Microscopy

Light sheet microscopy for fast functional imaging of 3D neuronal cultures in hydrogels

2020 ◽  
Author(s):  
Gustavo Castro-Olvera ◽  
Jorge Madrid-Wolff ◽  
Omar E. Olarte ◽  
Estefanía Estévez-Priego ◽  
Adriaan A. Ludl ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Neuronal Cultures ◽  
Light Sheet Microscopy

High-fidelity functional and structural whole-brain imaging with Bessel-beam light-sheet microscopy (Conference Presentation)

2017 ◽  
Author(s):  
Marie Caroline Müllenbroich ◽  
Ludovico Silvestri ◽  
Lapo Turrini ◽  
Antonino Paolo Di Giovanna ◽  
Tommaso Alterini ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Bessel Beam ◽  
Light Sheet ◽  
High Fidelity ◽  
Whole Brain ◽  
Light Sheet Microscopy

scholarly journals Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sota Takanezawa ◽  
Takashi Saitou ◽  
Takeshi Imamura
Keyword(s):  
High Speed ◽  
Bessel Beam ◽  
Light Sheet ◽  
Time Lapse ◽  
Whole Body ◽  
Wide Field ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Two Photon Excitation

AbstractTwo-photon excitation can lower phototoxicity and improve penetration depth, but its narrow excitation range restricts its applications in light-sheet microscopy. Here, we propose simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams. This unit can stretch the beam full width at half maximum of 600–1000 μm with less than a 4-μm waist when using a 10× illumination lens. A two-photon excitation digital scanned light-sheet microscope possessing this range of field of view and ~2–3-μm axial resolution is constructed and used to analyze the cellular dynamics over the whole body of medaka fish. We demonstrate long-term time-lapse observations over several days and high-speed recording with ~3 mm3 volume per 4 s of the embryos. Our system is minimal and suppresses laser power loss, which can broaden applications of two-photon excitation in light-sheet microscopy.

Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam

2018 ◽  
Vol 12 (1) ◽  
pp. e201800094 ◽  
Author(s):  
Hao Jia ◽  
Xianghua Yu ◽  
Yanlong Yang ◽  
Xing Zhou ◽  
Shaohui Yan ◽  
...  
Keyword(s):  
Bessel Beam ◽  
Resolution Enhancement ◽  
Light Sheet ◽  
Double Scanning ◽  
Axial Resolution ◽  
Light Sheet Microscopy

Increasing sensitivity and accuracy of brain-wide quantitative studies in light-sheet microscopy

2017 ◽  
Author(s):  
Caroline M. Müllenbroich ◽  
Ludovico Silvestri ◽  
Lapo Turrini ◽  
Tommaso Alterini ◽  
Antonino P. Di Giovanna ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Fold Increase ◽  
Structural Data ◽  
Light Sheet ◽  
High Fidelity ◽  
Random Errors ◽  
Bessel Beams ◽  
Light Sheet Microscopy ◽  
Quantitative Studies ◽  
Increase In Accuracy

AbstractLight-sheet microscopy (LSM) has proven a useful tool in neuroscience to image whole brains with high frame rates at cellular resolution. LSM is employed either in combination with tissue clearing to reconstruct the cyto-architecture over the entire mouse brain or with intrinsically transparent samples like zebrafish larvae for functional imaging. Inherently to LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest and, during functional imaging, modulate fluorescence variations related to neuronal activity. Here, we report how Bessel beams reduce streaking artifacts and produce high-fidelity structural data. Furthermore, using Bessel beams, we demonstrate a fivefold increase in sensitivity to calcium transients and a 20 fold increase in accuracy in the detection of activity correlations in functional imaging. Our results demonstrate the contamination of data by systematic and random errors through Gaussian illumination and furthermore quantify the increase in fidelity of such data when using Bessel beams.

Whole-brain functional imaging with two-photon light-sheet microscopy

2015 ◽  
Vol 12 (5) ◽  
pp. 379-380 ◽  
Author(s):  
Sébastien Wolf ◽  
Willy Supatto ◽  
Georges Debrégeas ◽  
Pierre Mahou ◽  
Sergei G Kruglik ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy

Functional Imaging with Light-Sheet Microscopy

2020 ◽  
pp. 21-54
Author(s):  
Raghav K. Chhetri ◽  
Philipp J. Keller
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy
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