High-speed wide-field optical-sectioning fluorescence microscopy based on one-shot structured illumination

2020 ◽  
Author(s):  
Zhong Zheng ◽  
Ruheng Shi ◽  
Lingjie Kong
Keyword(s):  
Fluorescence Microscopy ◽  
High Speed ◽  
Wide Field ◽  
Structured Illumination ◽  
Optical Sectioning

scholarly journals Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination

2008 ◽  
Vol 94 (12) ◽  
pp. 4957-4970 ◽  
Author(s):  
Mats G.L. Gustafsson ◽  
Lin Shao ◽  
Peter M. Carlton ◽  
C. J. Rachel Wang ◽  
Inna N. Golubovskaya ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Wide Field ◽  
Structured Illumination

scholarly journals Fast, Accurate Polarization and Polarity Imaging with Polarized Structured Illumination

2019 ◽  
Author(s):  
Karl Zhanghao ◽  
Wenhui Liu ◽  
Meiqi Li ◽  
Xingye Chen ◽  
Chunyan Shan ◽  
...  
Keyword(s):  
Live Cells ◽  
Polarization Modulation ◽  
Optical Transfer Function ◽  
Wide Field ◽  
Structured Illumination ◽  
Optical Sectioning ◽  
Polarization Imaging ◽  
Order Of Magnitude ◽  
Ratiometric Imaging ◽  
Optical Transfer

AbstractThe orientation and wobbling behavior of the fluorescent dipoles are of great significance in revealing the structure and state of cells. Due to the poor optical sectioning capability of wide-field microscopy, the polarization modulation signals are susceptible to the neighboring fluorophores. The missing cone of wide field optical transfer function induces vast out-of-focus background, resulting in biased polarization orientation and decrease polarization factor. Here, we apply polarized structured illumination to achieve polarization modulation imaging with optical sectioning, and simultaneously measure the lipid polarity with two-color ratiometric imaging. Our results demonstrate a significant increase in measurement accuracy of not only the dipole orientations but also the wobbling behavior of the ensemble dipole. Compared to the conventional confocal polarization imaging, our method obtains an order-of-magnitude faster imaging speed, capturing the fast dynamics of subcellular structures in live cells.

Localized plasmon assisted structured illumination microscopy for wide-field high-speed dispersion-independent super resolution imaging

Nanoscale ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 5807-5812 ◽  
Author(s):  
Joseph Louis Ponsetto ◽  
Feifei Wei ◽  
Zhaowei Liu
Keyword(s):  
Antenna Array ◽  
High Speed ◽  
Super Resolution ◽  
Fluorescent Imaging ◽  
Wide Field ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Resolution Imaging ◽  
Localized Plasmon ◽  
Imaging Resolution

Fluorescent imaging resolution down to 51 nm is shown by generating tunable localized plasmon excitations on a nano-antenna array.

scholarly journals Moxifloxacin based axially swept wide-field fluorescence microscopy for high-speed imaging of conjunctival goblet cells

2020 ◽  
Vol 11 (9) ◽  
pp. 4890
Author(s):  
Jungbin Lee ◽  
Seonghan Kim ◽  
Chang Ho Yoon ◽  
Myoung Joon Kim ◽  
Ki Hean Kim
Keyword(s):  
Fluorescence Microscopy ◽  
High Speed ◽  
Goblet Cells ◽  
Wide Field ◽  
High Speed Imaging ◽  
Conjunctival Goblet Cells

Wide-field high-resolution structured illumination solid immersion fluorescence microscopy

2011 ◽  
Vol 36 (15) ◽  
pp. 2794 ◽  
Author(s):  
Lin Wang ◽  
Mark C. Pitter ◽  
Michael G. Somekh
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Wide Field ◽  
Structured Illumination

scholarly journals Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Andreas Markwirth ◽  
Mario Lachetta ◽  
Viola Mönkemöller ◽  
Rainer Heintzmann ◽  
Wolfgang Hübner ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
High Speed ◽  
Resolution Enhancement ◽  
Optical Diffraction ◽  
Video Frame ◽  
Wide Field ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Reconstruction Software ◽  
Microscopy Techniques

Abstract Super-resolved structured illumination microscopy (SR-SIM) is among the fastest fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-build instruments are able to deliver two-fold resolution enhancement with high acquisition speed. SR-SIM is usually a two-step process, with raw-data acquisition and subsequent, time-consuming post-processing for image reconstruction. In contrast, wide-field and (multi-spot) confocal techniques produce high-resolution images instantly. Such immediacy is also possible with SR-SIM, by tight integration of a video-rate capable SIM with fast reconstruction software. Here we present instant SR-SIM by VIGOR (Video-rate Immediate GPU-accelerated Open-Source Reconstruction). We demonstrate multi-color SR-SIM at video frame-rates, with less than 250 ms delay between measurement and reconstructed image display. This is achieved by modifying and extending high-speed SR-SIM image acquisition with a new, GPU-enhanced, network-enabled image-reconstruction software. We demonstrate high-speed surveying of biological samples in multiple colors and live imaging of moving mitochondria as an example of intracellular dynamics.

scholarly journals Perfecting and extending the near-infrared biological window

2021 ◽  
Author(s):  
Zhe Feng ◽  
Tao Tang ◽  
Tianxiang Wu ◽  
Xiaoming Yu ◽  
Yuhuang Zhang ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Near Infrared ◽  
Wide Field ◽  
Positive Contribution ◽  
Optical Sectioning ◽  
Peak Emission Wavelength ◽  
In Vivo Fluorescence Imaging ◽  
Definition Of ◽  
Peak Emission

In vivo fluorescence imaging in the second near-infrared window (NIR-II) has been considered as a promising technique for visualizing the mammals. However, the definition of the NIR-II region and the mechanism accounting for the excellent performance still need to be perfected. Herein, we simulated bioimaging in the NIR spectral range (to 2340 nm), confirmed the positive contribution of moderate light absorption by water in intravital imaging and perfected the NIR-II window as 900-1880 nm, where the 1400-1500 nm was defined as NIR-IIx region and the 1700-1880 nm was defined as NIR-IIc region, respectively. Moreover, the 2080-2340 nm was newly proposed as the third near-infrared (NIR-III) window, which was believed to provide the best imaging quality. The wide-field fluorescence microscopy in brain, in addition, was performed around NIR-IIx region with excellent optical sectioning strength and the largest imaging depth of in vivo NIR-II fluorescence microscopy to date. We also proposed 1400 nm long-pass detection in off-peak NIR-II imaging whose profits exceeded those of NIR-IIb imaging, using bright fluorophores with short peak emission wavelength.

scholarly journals Perfecting and extending the near-infrared imaging window

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhe Feng ◽  
Tao Tang ◽  
Tianxiang Wu ◽  
Xiaoming Yu ◽  
Yuhuang Zhang ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Wide Field ◽  
Positive Contribution ◽  
Optical Sectioning ◽  
Photon Propagation ◽  
In Vivo Fluorescence Imaging ◽  
Definition Of

AbstractIn vivo fluorescence imaging in the second near-infrared window (NIR-II) has been considered as a promising technique for visualizing mammals. However, the definition of the NIR-II region and the mechanism accounting for the excellent performance still need to be perfected. Herein, we simulate the photon propagation in the NIR region (to 2340 nm), confirm the positive contribution of moderate light absorption by water in intravital imaging and perfect the NIR-II window as 900–1880 nm, where 1400–1500 and 1700–1880 nm are defined as NIR-IIx and NIR-IIc regions, respectively. Moreover, 2080–2340 nm is newly proposed as the third near-infrared (NIR-III) window, which is believed to provide the best imaging quality. The wide-field fluorescence microscopy in the brain is performed around the NIR-IIx region, with excellent optical sectioning strength and the largest imaging depth of intravital NIR-II fluorescence microscopy to date. We also propose 1400 nm long-pass detection in off-peak NIR-II imaging whose performance exceeds that of NIR-IIb imaging, using bright fluorophores with short emission wavelength.

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