Recent development in wavefront shaping-enabled optical focusing and its application towards deep-tissue single neuron stimulation

2020 ◽  
Author(s):  
Puxiang Lai ◽  
Tianting Zhong
Keyword(s):  
Single Neuron ◽  
Deep Tissue ◽  
Wavefront Shaping ◽  
Optical Focusing

scholarly journals Quantum reference beacon–guided superresolution optical focusing in complex media

Science ◽  
2019 ◽  
Vol 363 (6426) ◽  
pp. 528-531 ◽  
Author(s):  
Donggyu Kim ◽  
Dirk R. Englund
Keyword(s):  
Quantum Information ◽  
Quantum Information Processing ◽  
Optical Excitation ◽  
Optical Scattering ◽  
Nitrogen Vacancy ◽  
Complex Media ◽  
Deep Tissue ◽  
Nitrogen Vacancy Centers ◽  
Wavefront Shaping ◽  
Optical Focusing

Optical scattering is generally considered to be a nuisance of microscopy that limits imaging depth and spatial resolution. Wavefront shaping techniques enable optical imaging at unprecedented depth, but attaining superresolution within complex media remains a challenge. We used a quantum reference beacon (QRB), consisting of solid-state quantum emitters with spin-dependent fluorescence, to provide subwavelength guidestar feedback for wavefront shaping to achieve a superresolution optical focus. We implemented the QRB-guided imaging with nitrogen-vacancy centers in diamond nanocrystals, which enable optical focusing with a subdiffraction resolution below 186 nanometers (less than half the wavelength). QRB-assisted wavefront-shaping should find use in a range of applications, including deep-tissue quantum enhanced sensing and individual optical excitation of magnetically coupled spin ensembles for applications in quantum information processing.

scholarly journals High-speed single-shot optical focusing through dynamic scattering media with full-phase wavefront shaping

2017 ◽  
Vol 111 (22) ◽  
pp. 221109 ◽  
Author(s):  
Ashton S. Hemphill ◽  
Yuecheng Shen ◽  
Yan Liu ◽  
Lihong V. Wang
Keyword(s):  
High Speed ◽  
Single Shot ◽  
Scattering Media ◽  
Wavefront Shaping ◽  
Optical Focusing ◽  
Dynamic Scattering

Reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Lejia Hu ◽  
Shuwen Hu ◽  
Younong Li ◽  
Wei Gong ◽  
Ke Si
Keyword(s):  
Deep Tissue ◽  
Adaptive Technique ◽  
Wavefront Shaping

scholarly journals Deep tissue scattering compensation with three-photon F-SHARP

2021 ◽  
Author(s):  
Caroline Berlage ◽  
Malinda L. S. Tantirigama ◽  
Mathias Babot ◽  
Diego Di Battista ◽  
Clarissa Whitmire ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Dendritic Spines ◽  
Imaging Techniques ◽  
Biological Research ◽  
Biological Applications ◽  
Deep Tissue ◽  
Two Photon ◽  
Tissue Scattering ◽  
Compensation Technique ◽  
Wavefront Shaping

Optical imaging techniques are widely used in biological research, but their penetration depth is limited by tissue scattering. Wavefront shaping techniques are able to overcome this problem in principle, but are often slow and their performance depends on the sample. This greatly reduces their practicability for biological applications. Here we present a scattering compensation technique based on three-photon (3P) excitation, which converges faster than comparable two-photon (2P) techniques and works reliably even on densely labeled samples, where 2P approaches fail. To demonstrate its usability and advantages for biomedical imaging we apply it to the imaging of dendritic spines on GFP-labeled layer 5 neurons in an anesthetized mouse.

scholarly journals Photoacoustically guided wavefront shaping for enhanced optical focusing in scattering media

2015 ◽  
Vol 9 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Puxiang Lai ◽  
Lidai Wang ◽  
Jian Wei Tay ◽  
Lihong V. Wang
Keyword(s):  
Scattering Media ◽  
Wavefront Shaping ◽  
Optical Focusing

scholarly journals Overcoming the penetration depth limit in optical microscopy: Adaptive optics and wavefront shaping

2019 ◽  
Vol 12 (04) ◽  
pp. 1930002 ◽  
Author(s):  
Cheolwoo Ahn ◽  
Byungjae Hwang ◽  
Kibum Nam ◽  
Hyungwon Jin ◽  
Taeseong Woo ◽  
...  
Keyword(s):  
High Resolution ◽  
Optical Microscopy ◽  
Adaptive Optics ◽  
Single Cells ◽  
Tissue Imaging ◽  
Future Research ◽  
Deep Tissue ◽  
Depth Limit ◽  
Deep Tissue Imaging ◽  
Wavefront Shaping

Despite the unique advantages of optical microscopy for molecular specific high resolution imaging of living structure in both space and time, current applications are mostly limited to research settings. This is due to the aberrations and multiple scattering that is induced by the inhomogeneous refractive boundaries that are inherent to biological systems. However, recent developments in adaptive optics and wavefront shaping have shown that high resolution optical imaging is not fundamentally limited only to the observation of single cells, but can be significantly enhanced to realize deep tissue imaging. To provide insight into how these two closely related fields can expand the limits of bio imaging, we review the recent progresses in their performance and applicable range of studies as well as potential future research directions to push the limits of deep tissue imaging.

scholarly journals Time-reversed optical focusing through scattering media by digital full phase and amplitude recovery using a single phase-only SLM

2015 ◽  
Vol 08 (02) ◽  
pp. 1550007 ◽  
Author(s):  
Qiang Yang ◽  
Xinzhu Sang ◽  
Daxiong Xu
Keyword(s):  
Single Phase ◽  
Scattered Light ◽  
Incident Light ◽  
Scattering Media ◽  
Ballistic Regime ◽  
Light Modulator ◽  
Speckle Field ◽  
Biomedical Optical Imaging ◽  
Wavefront Shaping ◽  
Optical Focusing

Focusing light though scattering media beyond the ballistic regime is a challenging task in biomedical optical imaging. This challenge can be overcome by wavefront shaping technique, in which a time-reversed (TR) wavefront of scattered light is generated to suppress the scattering. In previous TR optical focusing experiments, a phase-only spatial light modulator (SLM) has been typically used to control the wavefront of incident light. Unfortunately, although the phase information is reconstructed by the phase-only SLM, the amplitude information is lost, resulting in decreased peak-to-background ratio (PBR) of optical focusing in the TR wavefront reconstruction. A new method of TR optical focusing through scattering media is proposed here, which numerically reconstructs the full phase and amplitude of a simulated scattered light field by using a single phase-only SLM. Simulation results and the proposed optical setup show that the time-reversal of a fully developed speckle field can be digitally implemented with both phase and amplitude recovery, affording a way to improve the performance of light focusing through scattering media.

scholarly journals Focusing light into scattering media with ultrasound-induced field perturbation

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhongtao Cheng ◽  
Lihong V. Wang
Keyword(s):  
High Speed ◽  
Superior Performance ◽  
Single Shot ◽  
Scattering Media ◽  
Zeroth Order ◽  
Field Perturbation ◽  
Induced Field ◽  
Wavefront Shaping ◽  
Flexible Mechanism ◽  
Optical Focusing

AbstractFocusing light into scattering media, although challenging, is highly desirable in many realms. With the invention of time-reversed ultrasonically encoded (TRUE) optical focusing, acousto-optic modulation was demonstrated as a promising guidestar mechanism for achieving noninvasive and addressable optical focusing into scattering media. Here, we report a new ultrasound-assisted technique, ultrasound-induced field perturbation optical focusing, abbreviated as UFP. Unlike in conventional TRUE optical focusing, where only the weak frequency-shifted first-order diffracted photons due to acousto-optic modulation are useful, here UFP leverages the brighter zeroth-order photons diffracted by an ultrasonic guidestar as information carriers to guide optical focusing. We find that the zeroth-order diffracted photons, although not frequency-shifted, do have a field perturbation caused by the existence of the ultrasonic guidestar. By detecting and time-reversing the differential field of the frequency-unshifted photons when the ultrasound is alternately ON and OFF, we can focus light to the position where the field perturbation occurs inside the scattering medium. We demonstrate here that UFP optical focusing has superior performance to conventional TRUE optical focusing, which benefits from the more intense zeroth-order photons. We further show that UFP optical focusing can be easily and flexibly developed into double-shot realization or even single-shot realization, which is desirable for high-speed wavefront shaping. This new method upsets conventional thinking on the utility of an ultrasonic guidestar and broadens the horizon of light control in scattering media. We hope that it provides a more efficient and flexible mechanism for implementing ultrasound-guided wavefront shaping.

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