Reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Lejia Hu ◽  
Shuwen Hu ◽  
Younong Li ◽  
Wei Gong ◽  
Ke Si
Keyword(s):  
Deep Tissue ◽  
Adaptive Technique ◽  
Wavefront Shaping

scholarly journals Quantum reference beacon–guided superresolution optical focusing in complex media

Science ◽  
2019 ◽  
Vol 363 (6426) ◽  
pp. 528-531 ◽  
Author(s):  
Donggyu Kim ◽  
Dirk R. Englund
Keyword(s):  
Quantum Information ◽  
Quantum Information Processing ◽  
Optical Excitation ◽  
Optical Scattering ◽  
Nitrogen Vacancy ◽  
Complex Media ◽  
Deep Tissue ◽  
Nitrogen Vacancy Centers ◽  
Wavefront Shaping ◽  
Optical Focusing

Optical scattering is generally considered to be a nuisance of microscopy that limits imaging depth and spatial resolution. Wavefront shaping techniques enable optical imaging at unprecedented depth, but attaining superresolution within complex media remains a challenge. We used a quantum reference beacon (QRB), consisting of solid-state quantum emitters with spin-dependent fluorescence, to provide subwavelength guidestar feedback for wavefront shaping to achieve a superresolution optical focus. We implemented the QRB-guided imaging with nitrogen-vacancy centers in diamond nanocrystals, which enable optical focusing with a subdiffraction resolution below 186 nanometers (less than half the wavelength). QRB-assisted wavefront-shaping should find use in a range of applications, including deep-tissue quantum enhanced sensing and individual optical excitation of magnetically coupled spin ensembles for applications in quantum information processing.

scholarly journals Deep tissue scattering compensation with three-photon F-SHARP

2021 ◽  
Author(s):  
Caroline Berlage ◽  
Malinda L. S. Tantirigama ◽  
Mathias Babot ◽  
Diego Di Battista ◽  
Clarissa Whitmire ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Dendritic Spines ◽  
Imaging Techniques ◽  
Biological Research ◽  
Biological Applications ◽  
Deep Tissue ◽  
Two Photon ◽  
Tissue Scattering ◽  
Compensation Technique ◽  
Wavefront Shaping

Optical imaging techniques are widely used in biological research, but their penetration depth is limited by tissue scattering. Wavefront shaping techniques are able to overcome this problem in principle, but are often slow and their performance depends on the sample. This greatly reduces their practicability for biological applications. Here we present a scattering compensation technique based on three-photon (3P) excitation, which converges faster than comparable two-photon (2P) techniques and works reliably even on densely labeled samples, where 2P approaches fail. To demonstrate its usability and advantages for biomedical imaging we apply it to the imaging of dendritic spines on GFP-labeled layer 5 neurons in an anesthetized mouse.

scholarly journals Overcoming the penetration depth limit in optical microscopy: Adaptive optics and wavefront shaping

2019 ◽  
Vol 12 (04) ◽  
pp. 1930002 ◽  
Author(s):  
Cheolwoo Ahn ◽  
Byungjae Hwang ◽  
Kibum Nam ◽  
Hyungwon Jin ◽  
Taeseong Woo ◽  
...  
Keyword(s):  
High Resolution ◽  
Optical Microscopy ◽  
Adaptive Optics ◽  
Single Cells ◽  
Tissue Imaging ◽  
Future Research ◽  
Deep Tissue ◽  
Depth Limit ◽  
Deep Tissue Imaging ◽  
Wavefront Shaping

Despite the unique advantages of optical microscopy for molecular specific high resolution imaging of living structure in both space and time, current applications are mostly limited to research settings. This is due to the aberrations and multiple scattering that is induced by the inhomogeneous refractive boundaries that are inherent to biological systems. However, recent developments in adaptive optics and wavefront shaping have shown that high resolution optical imaging is not fundamentally limited only to the observation of single cells, but can be significantly enhanced to realize deep tissue imaging. To provide insight into how these two closely related fields can expand the limits of bio imaging, we review the recent progresses in their performance and applicable range of studies as well as potential future research directions to push the limits of deep tissue imaging.

Healing effects of curcumin nanoparticles in deep tissue injury mouse model.

2020 ◽  
Vol 18 ◽  
Author(s):  
Zirui Zhang ◽  
Shangcong Han ◽  
Panpan Liu ◽  
Xu Yang ◽  
Jing Han ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Tissue Injury ◽  
Inflammatory Cells ◽  
Pcr Analysis ◽  
Protective Effects ◽  
Deep Tissue ◽  
Deep Tissue Injury

Background: Chronic inflammation and lack of angiogenesis are the important pathological mechanisms in deep tissue injury (DTI). Curcumin is a well-known anti-inflammatory and antioxidant agent. However, curcumin is unstable under acidic and alkaline conditions, and can be rapidly metabolized and excreted in the bile, which shortens its bioactivity and efficacy. Objective: This study aimed to prepare curcumin-loaded poly (lactic-co-glycolic acid) nanoparticles (CPNPs) and to elucidate the protective effects and underlying mechanisms of wound healing in DTI models. Methods: CPNPs were evaluated for particle size, biocompatibility, in vitro drug release and their effect on in vivo wound healing. Results : The results of in vivo wound closure analysis revealed that CPNP treatments significantly improved wound contraction rates (p<0.01) at a faster rate than other three treatment groups. H&E staining revealed that CPNP treatments resulted in complete epithelialization and thick granulation tissue formation, whereas control groups resulted in a lack of compact epithelialization and persistence of inflammatory cells within the wound sites. Quantitative real-time PCR analysis showed that treatment with CPNPs suppressed IL-6 and TNF-α mRNA expression, and up-regulated TGF-β, VEGF-A and IL-10 mRNA expression. Western blot analysis showed up-regulated protein expression of TGF-β, VEGF-A and phosphorylatedSTAT3. Conclusion: Our results showed that CPNPs enhanced wound healing in DTI models, through modulation of the JAK2/STAT3 signalling pathway and subsequent upregulation of pro-healing factors.

Sign in / Sign up

Export Citation Format

Share Document