Cortisol detection in saliva using thermography in dot immunoassay

2020 ◽  
Author(s):  
Elizaveta Panfilova ◽  
Kseniya Sharova
Keyword(s):  
Dot Immunoassay

scholarly journals Semi-quantitative dot immunoassay for detection of IgM anti-dengue antibodies in human sera

2000 ◽  
pp. 9
Author(s):  
Agus Sjahrurachman ◽  
Betty Ernawati ◽  
Fera Ibrahim ◽  
Mardiastuti Mardiastuti ◽  
Tjahjani M. Sudiro ◽  
...  
Keyword(s):  
Human Sera ◽  
Dot Immunoassay

A rapid dot immunoassay for detecting the Brazilian Purpuric Fever clone of Haemophilus influenzae biogroup aegyptius with a ?flow through? device

1995 ◽  
Vol 30 (6) ◽  
pp. 345-349 ◽  
Author(s):  
Gloria W. Ajello ◽  
Ghassan M. Matar ◽  
B. Swaminathan ◽  
William F. Bibb ◽  
Leta O. Helsel ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Brazilian Purpuric Fever ◽  
Flow Through ◽  
Dot Immunoassay

A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Karsten Conrad ◽  
Dirk Roggenbuck ◽  
Annelore Ittenson ◽  
Dirk Reinhold ◽  
Thomas Buettner ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Simultaneous Detection ◽  
Serum Levels ◽  
Iga Deficiency ◽  
Enzyme Linked Immunosorbent Assay ◽  
Iga Antibodies ◽  
Work Up ◽  
Simultaneous Assessment ◽  
Dot Immunoassay

AbstractThis study investigated whether a dot immunoassay (DIA) can provide simultaneous detection of anti-tissue transglutaminase (tTG), anti-deamidated gliadin (DG) and total IgA antibodies, as required in the work-up of celiac disease (CD) patients.Celiac disease patients (n=111) consecutively diagnosed from 2001 to 2011 at the Children’s Hospital and Institute of Immunology (Technical University Dresden) were tested for anti-tTG, anti-DG and total IgA by enzyme-linked immunosorbent assay (ELISA) and DIA retrospectively. Blood donors (n=45) and non-CD individuals with low IgA serum levels (n=8) were included as controls. Antibodies to endomysial antigens (EmA) were assessed by indirect immunofluorescence (IIF).Four (3.6%) of 111 CD patients demonstrated an IgA deficiency with total IgA below 50 mg/L by ELISA. Total IgA of the 107 IgA-non-deficient CD patients varied from 70 to 6000 mg/L. All four IgA-deficient CD patients were detected by a reduced reaction control of DIA and demonstrated positive anti-tTG or anti-DG IgG by DIA or ELISA. Detection of anti-tTG and anti-DG by DIA and ELISA showed a very good agreement (IgA: κ=0.972, 0.856, respectively; IgG: 0.921, 0.895, respectively).Immunodot assay is a reliable and easy-to-use technique for the detection of IgA-deficient CD patients. Simultaneous assessment of anti-tTG and anti-DG IgA antibodies, and IgA deficiency by DIA can improve the efficacy of CD serology.

An evaluation of dipstick-dot immunoassay in the detection of antibodies to HIV-1 and 2 in Zimbabwe

1997 ◽  
Vol 2 (1) ◽  
pp. 83-88 ◽  
Author(s):  
C. Ray ◽  
Peter Mason ◽  
Heather Smith ◽  
Luanne Rogers ◽  
Ocean Tobaiwa ◽  
...  
Keyword(s):  
Hiv 1 ◽  
Dot Immunoassay

scholarly journals Development of the scheme of obtaining antibodies to the ribonucleoprotein of attenuated rabies virus

2019 ◽  
pp. 3-8 ◽  
Author(s):  
Yu. K. Gavrilova ◽  
S. V. Generalov ◽  
M. N. Kireev ◽  
N. A. Sharapova ◽  
E. G. Abramova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Rabies Virus ◽  
Antibody Titer ◽  
Colloidal Gold ◽  
Target Component ◽  
High Antibody ◽  
Experimental Animals ◽  
Colloidal Gold Nanoparticles ◽  
High Antibody Titer ◽  
Dot Immunoassay

Aim. Isolation of ribonucleoprotein (RNP) of an attenuated rabies virus, develop schemes for immunizing animals with RNP-based preparations and determine the most effective scheme that allows obtaining serum with a high antibody titer to the RNP.Materials and methods. We used the transplantable cell line Vero, the strain rabies virus «Moscow 3253 Vero», adapted for reproduction on Vero, rabbits of the chinchilla breed. In order to obtain serums containing antibodies to the RNP of the rabies virus, experimental schemes have been proposed for immunizing animals with RNP, including with adjuvants: polyoxidonium and colloidal gold. The dynamics of the accumulation of antibodies to the RNP of the rabies virus in the blood serum of experimental animals was studied by dot-immunoassay.Results. The target component (RNP of the rabies virus) was isolated directly from the cytoplasm of the Vero cell culture infected with the rabies virus according to the modified M. Dastkhosh (2014) method, lyophilized and used in the development of preparations for immunizing experimental animals. In the study of the dynamics of the formation of antibodies to RNP of the rabies virus by the method of dot-immunoassay, the effectiveness of an adjuvant is established — colloidal gold nanoparticles ranging in size from 15 to 17 nm, the use of which makes it possible to increase the antibody titer by 2 times.Conclusion. The results obtained are of interest for further research related to the design of diagnostic products and the development of methodological techniques using such preparations.

An amplified dot immunoassay for the direct quantitation of adapted and wild strains of human cytomegalovirus

1989 ◽  
Vol 24 (3) ◽  
pp. 327-334 ◽  
Author(s):  
M. Musiani ◽  
M. Zerbini ◽  
G. Gentilomi ◽  
M. Plazzi ◽  
G. Gallinella ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Wild Strains ◽  
Dot Immunoassay

Multiplexed dot immunoassay using Ag nanocubes, Au/Ag alloy nanoparticles, and Au/Ag nanocages

Nano Research ◽  
2012 ◽  
Vol 5 (2) ◽  
pp. 124-134 ◽  
Author(s):  
Elizaveta Panfilova ◽  
Alexander Shirokov ◽  
Boris Khlebtsov ◽  
Larisa Matora ◽  
Nikolai Khlebtsov
Keyword(s):  
Alloy Nanoparticles ◽  
Dot Immunoassay
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