Polarization interferometry of the polycrystalline structure of biological tissues

2020 ◽  
Author(s):  
Olexander V. Dubolazov ◽  
O. Pavlyukovitch ◽  
N. Pavlyukovitch ◽  
O. Gurina ◽  
A. Arkhelyuk
Keyword(s):  
Biological Tissues ◽  
Polycrystalline Structure ◽  
Polarization Interferometry

scholarly journals Stokes-Correlometry Analysis of Biological Tissues With Polycrystalline Structure

2019 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Alexander Ushenko ◽  
Anton Sdobnov ◽  
Alexander Dubolazov ◽  
Marta Grytsiuk ◽  
Yuriy Ushenko ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Polycrystalline Structure

scholarly journals Polarization-interference mapping of networks in diffusal polycristaline biological tissues

2019 ◽  
Keyword(s):  
Experimental Testing ◽  
Physiological State ◽  
Morphological Structure ◽  
Biological Tissues ◽  
Mapping Method ◽  
Polycrystalline Structure ◽  
Matrix Mapping ◽  
Stokes Polarimetry ◽  
Rates Of Growth ◽  
Holographic Reconstruction

Objectives: Development and experimental testing of the complex of Stokes-polarimetry and interferometry methods using algorithms for digital holographic reconstruction of the amplitude-phase structure of object fields for obtaining interrelationships between 3D distributions of depolarization maps and peculiarities of the polycrystalline structure of histological sections of biological tissues of different morphological structures and physiological state. Materials and methods: The basis of the 3D Müller-matrix mapping method is the use of a reference wave of laser radiation, which is superimposed on a polarization-non-uniform image of the biological layer in the scheme of the optical interferometer. Results: In the process of comparative analysis of the map of depolarization of biological tissues with different geometric scales of the morphological structure, we found different rates of growth of the degree of depolarization.

scholarly journals Differential Mueller matrix imaging of partially depolarizing optically anisotropic biological tissues

2019 ◽  
Vol 35 (4) ◽  
pp. 877-891 ◽  
Author(s):  
L. Trifonyuk ◽  
A. Sdobnov ◽  
W. Baranowski ◽  
V. Ushenko ◽  
O. Olar ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Mueller Matrix ◽  
Differential Diagnostics ◽  
Matrix Elements ◽  
Polycrystalline Structure ◽  
First Order ◽  
Histological Sections ◽  
Laser Polarimetry ◽  
Matrix Mapping ◽  
Differential Matrix

AbstractSince recently, a number of innovative polarization-based optical imaging modalities have been introduced and extensively used in various biomedical applications, with an ultimate aim to attain the practical tool for the optical biopsy and functional characterization of biological tissues. The techniques utilize polarization properties of light and Mueller matrix mapping of microscopic images of histological sections of biological tissues or polycrystalline films of biological fluids. The main drawback of currently developed laser polarimetry approaches and Mueller matrix mapping techniques is poor reproducibility of experimental data. This is due to azimuthal dependence of polarization and ellipticity values of most matrix elements to sample orientation in respect to incidence light polarization. Current study aims to generalize the methods of laser polarimetry for diagnosis of partially depolarizing optically anisotropic biological tissues. A method of differential Mueller matrix mapping for reconstruction of linear and circular birefringence and dichroism parameter distributions of partially depolarizing layers of biological tissues of different morphological structure is introduced and practically implemented. The coordinate distributions of the value of the first-order differential matrix elements of histological sections of brain tissue with spatially structured, optically anisotropic fibrillar network, as well as of parenchymatous tissue of the rectum wall with an “islet” polycrystalline structure are determined. Within the statistical analysis of polarization reproduced distributions of the averaged parameters of phase and amplitude anisotropy, the significant sensitivity of the statistical moments of the third and fourth orders to changes in the polycrystalline structure of partially depolarizing layers of biological tissue is observed. The differentiation of female reproductive sphere connective tissue is realized with excellent accuracy. The differential Mueller matrix mapping method for reconstruction of distributions of linear and circular birefringence and dichroism parameters of partially depolarizing layers of biological tissues of different morphological structures is proposed and substantiated. Differential diagnostics of changes in the phase (good balanced accuracy) and amplitude (excellent balanced accuracy) of the anisotropy of the partially depolarizing layers of the vagina wall tissue with prolapse of the genitals is realized. The maximum diagnostic efficiency of the first-order differential matrix method was demonstrated in comparison with the traditional methods of polarization and Mueller matrix mapping of histological sections of light-scattering biological tissues.

Polarization-phase reconstruction of polycrystalline structure of biological tissues

2019 ◽  
Author(s):  
Olexander V. Dubolazov ◽  
O. Olar ◽  
L. Pidkamin ◽  
A. Arkhelyuk ◽  
A. Motrich ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Phase Reconstruction ◽  
Polycrystalline Structure ◽  
Polarization Phase

3D Stokes correlometry of the polycrystalline structure of biological tissues

2020 ◽  
Author(s):  
Olexander V. Dubolazov ◽  
A. Bodnar ◽  
A. Pavlyukovich ◽  
N. Pavlyukovich ◽  
A. Ushenko ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Polycrystalline Structure

Three-Dimensional Visualization of the T-System of Frog Muscle Using High Voltage Electron Microscopy and a Lanthanum Stain

1977 ◽  
Vol 35 ◽  
pp. 570-571 ◽  
Author(s):  
Lee D. Peachey ◽  
Clara Franzini-Armstrong
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Three Dimensional ◽  
Biological Tissues ◽  
High Voltage Electron Microscopy ◽  
Transverse Tubular System ◽  
Peroxidase Reaction ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
T System

The effective study of biological tissues in thick slices of embedded material by high voltage electron microscopy (HVEM) requires highly selective staining of those structures to be visualized so that they are not hidden or obscured by other structures in the image. A tilt pair of micrographs with subsequent stereoscopic viewing can be an important aid in three-dimensional visualization of these images, once an appropriate stain has been found. The peroxidase reaction has been used for this purpose in visualizing the T-system (transverse tubular system) of frog skeletal muscle by HVEM (1). We have found infiltration with lanthanum hydroxide to be particularly useful for three-dimensional visualization of certain aspects of the structure of the T- system in skeletal muscles of the frog. Specifically, lanthanum more completely fills the lumen of the tubules and is denser than the peroxidase reaction product.

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