Quantification of fiber orientation uncertainty in polarized light imaging of the human brain

2020 ◽  
Author(s):  
Daniel Schmitz ◽  
Thomas Lippert ◽  
Katrin Amunts ◽  
Markus Axer
Keyword(s):  
Human Brain ◽  
Fiber Orientation ◽  
Polarized Light ◽  
Polarized Light Imaging

scholarly journals High-Resolution Fiber Tract Reconstruction in the Human Brain by Means of Three-Dimensional Polarized Light Imaging

2011 ◽  
Vol 5 ◽  
Author(s):  
Markus Axer ◽  
David Grässel ◽  
Melanie Kleiner ◽  
Jürgen Dammers ◽  
Timo Dickscheid ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Three Dimensional ◽  
Polarized Light ◽  
Fiber Tract ◽  
Polarized Light Imaging

scholarly journals Derivation of Fiber Orientations From Oblique Views Through Human Brain Sections in 3D-Polarized Light Imaging

2018 ◽  
Vol 12 ◽  
Author(s):  
Daniel Schmitz ◽  
Sascha E. A. Muenzing ◽  
Martin Schober ◽  
Nicole Schubert ◽  
Martina Minnerop ◽  
...  
Keyword(s):  
Human Brain ◽  
Polarized Light ◽  
Polarized Light Imaging

scholarly journals Analytical and fast Fiber Orientation Distribution reconstruction in 3D-Polarized Light Imaging

2020 ◽  
Vol 65 ◽  
pp. 101760 ◽  
Author(s):  
Abib Alimi ◽  
Samuel Deslauriers-Gauthier ◽  
Felix Matuschke ◽  
Andreas Müller ◽  
Sascha E.A. Muenzing ◽  
...  
Keyword(s):  
Fiber Orientation ◽  
Polarized Light ◽  
Orientation Distribution ◽  
Fiber Orientation Distribution ◽  
Fast Fiber ◽  
Polarized Light Imaging

Polarized light imaging of the human brain: a new approach to the data analysis of tilted sections

2014 ◽  
Author(s):  
Hendrik Wiese ◽  
David Gräßel ◽  
Uwe Pietrzyk ◽  
Katrin Amunts ◽  
Markus Axer
Keyword(s):  
Data Analysis ◽  
Human Brain ◽  
Polarized Light ◽  
New Approach ◽  
Polarized Light Imaging

scholarly journals Kinetochore-driven outgrowth of microtubules is a central contributor to kinetochore fiber maturation in crane-fly spermatocytes

2014 ◽  
Vol 25 (9) ◽  
pp. 1437-1445 ◽  
Author(s):  
James R. LaFountain ◽  
Rudolf Oldenbourg
Keyword(s):  
Liquid Crystal ◽  
Life Histories ◽  
Polarized Light ◽  
Fiber Formation ◽  
Image Brightness ◽  
Early Stages ◽  
Anaphase Onset ◽  
Bipolar Spindles ◽  
Polarized Light Imaging

We use liquid crystal polarized light imaging to record the life histories of single kinetochore (K-) fibers in living crane-fly spermatocytes, from their origins as nascent K-fibers in early prometaphase to their fully matured form at metaphase, just before anaphase onset. Increased image brightness due to increased retardance reveals where microtubules are added during K-fiber formation. Analysis of experimentally generated bipolar spindles with only one centrosome, as well as of regular, bicentrosomal spindles, reveals that microtubule addition occurs at the kinetochore-proximal ends of K-fibers, and added polymer expands poleward, giving rise to the robust K-fibers of metaphase cells. These results are not compatible with a model for K-fiber formation in which microtubules are added to nascent fibers solely by repetitive “search and capture” of centrosomal microtubule plus ends. Our interpretation is that capture of centrosomal microtubules—when deployed—is limited to early stages in establishment of nascent K-fibers, which then mature through kinetochore-driven outgrowth. When kinetochore capture of centrosomal microtubules is not used, the polar ends of K-fibers grow outward from their kinetochores and usually converge to make a centrosome-free pole.

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