scholarly journals 3D Polarized Light Imaging Portrayed: Visualization of Fiber Architecture Derived from 3D-PLI

2018 ◽  
Author(s):  
Nicole Schubert ◽  
Markus Axer ◽  
Uwe Pietrzyk ◽  
Katrin Amunts
Keyword(s):  
Polarized Light ◽  
Fiber Architecture ◽  
Polarized Light Imaging

scholarly journals Kinetochore-driven outgrowth of microtubules is a central contributor to kinetochore fiber maturation in crane-fly spermatocytes

2014 ◽  
Vol 25 (9) ◽  
pp. 1437-1445 ◽  
Author(s):  
James R. LaFountain ◽  
Rudolf Oldenbourg
Keyword(s):  
Liquid Crystal ◽  
Life Histories ◽  
Polarized Light ◽  
Fiber Formation ◽  
Image Brightness ◽  
Early Stages ◽  
Anaphase Onset ◽  
Bipolar Spindles ◽  
Polarized Light Imaging

We use liquid crystal polarized light imaging to record the life histories of single kinetochore (K-) fibers in living crane-fly spermatocytes, from their origins as nascent K-fibers in early prometaphase to their fully matured form at metaphase, just before anaphase onset. Increased image brightness due to increased retardance reveals where microtubules are added during K-fiber formation. Analysis of experimentally generated bipolar spindles with only one centrosome, as well as of regular, bicentrosomal spindles, reveals that microtubule addition occurs at the kinetochore-proximal ends of K-fibers, and added polymer expands poleward, giving rise to the robust K-fibers of metaphase cells. These results are not compatible with a model for K-fiber formation in which microtubules are added to nascent fibers solely by repetitive “search and capture” of centrosomal microtubule plus ends. Our interpretation is that capture of centrosomal microtubules—when deployed—is limited to early stages in establishment of nascent K-fibers, which then mature through kinetochore-driven outgrowth. When kinetochore capture of centrosomal microtubules is not used, the polar ends of K-fibers grow outward from their kinetochores and usually converge to make a centrosome-free pole.

Quantitative comparison of human myocardial fiber orientations derived from DTI and polarized light imaging

2018 ◽  
Vol 63 (21) ◽  
pp. 215003 ◽  
Author(s):  
Feng Yang ◽  
Yue-Min Zhu ◽  
Gabrielle Michalowicz ◽  
Pierre-Simon Jouk ◽  
Laurent Fanton ◽  
...  
Keyword(s):  
Polarized Light ◽  
Quantitative Comparison ◽  
Myocardial Fiber ◽  
Polarized Light Imaging

Grazing incidence polarized light imaging of footwear prints

2019 ◽  
Vol 20 (11) ◽  
pp. 1543-1550
Author(s):  
Xin-yi Bi ◽  
Rui-fang Han ◽  
Ran Liao ◽  
Wu-sheng Feng ◽  
Da Li ◽  
...  
Keyword(s):  
Polarized Light ◽  
Grazing Incidence ◽  
Polarized Light Imaging

A cortex-like canonical circuit in the avian forebrain

Science ◽  
2020 ◽  
Vol 369 (6511) ◽  
pp. eabc5534
Author(s):  
Martin Stacho ◽  
Christina Herold ◽  
Noemi Rook ◽  
Hermann Wagner ◽  
Markus Axer ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Cognitive Skills ◽  
Three Dimensional ◽  
Polarized Light ◽  
Structural Basis ◽  
Fiber Architecture ◽  
Neuronal Circuitry ◽  
Mammalian Counterpart ◽  
Tracing Techniques ◽  
Neuronal Computation

Although the avian pallium seems to lack an organization akin to that of the cerebral cortex, birds exhibit extraordinary cognitive skills that are comparable to those of mammals. We analyzed the fiber architecture of the avian pallium with three-dimensional polarized light imaging and subsequently reconstructed local and associative pallial circuits with tracing techniques. We discovered an iteratively repeated, column-like neuronal circuitry across the layer-like nuclear boundaries of the hyperpallium and the sensory dorsal ventricular ridge. These circuits are connected to neighboring columns and, via tangential layer-like connections, to higher associative and motor areas. Our findings indicate that this avian canonical circuitry is similar to its mammalian counterpart and might constitute the structural basis of neuronal computation.

scholarly journals Polarized light microscopy for 3-dimensional mapping of collagen fiber architecture in ocular tissues

2018 ◽  
Vol 11 (8) ◽  
pp. e201700356 ◽  
Author(s):  
Bin Yang ◽  
Ning-Jiun Jan ◽  
Bryn Brazile ◽  
Andrew Voorhees ◽  
Kira L. Lathrop ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Collagen Fiber ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Fiber Architecture ◽  
Ocular Tissues ◽  
3 Dimensional ◽  
Dimensional Mapping

scholarly journals Evaluation of collimated polarized light imaging for real-time intraoperative selective nerve identification in the human hand

2017 ◽  
Vol 8 (9) ◽  
pp. 4122 ◽  
Author(s):  
K. W. T. K Chin ◽  
A. F. Engelsman ◽  
P. T. K. Chin ◽  
S. L. Meijer ◽  
S. D. Strackee ◽  
...  
Keyword(s):  
Real Time ◽  
Polarized Light ◽  
Human Hand ◽  
Nerve Identification ◽  
Polarized Light Imaging

Polarized light imaging of object hidden in scattering media: model experiments

2001 ◽  
Author(s):  
Yurii P. Sinichkin ◽  
Dmitry A. Zimnyakov ◽  
Vadim V. Giterman
Keyword(s):  
Polarized Light ◽  
Scattering Media ◽  
Model Experiments ◽  
Polarized Light Imaging
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