Super-resolution Raman Microscopy by selective suppression of stimulated Raman scattering (Conference Presentation)

2020 ◽  
Author(s):  
Sang-Hee Shim
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Super Resolution ◽  
Raman Microscopy ◽  
Selective Suppression ◽  
Stimulated Raman

scholarly journals Super-resolution vibrational imaging using expansion stimulated Raman scattering microscopy

2021 ◽  
Author(s):  
Lixue Shi ◽  
Aleksandra Klimas ◽  
Brendan Gallagher ◽  
Zhangyu Cheng ◽  
Feifei Fu ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Mouse Brain ◽  
Stimulated Raman Scattering ◽  
Super Resolution ◽  
Chemical Imaging ◽  
Metabolic Imaging ◽  
Label Free ◽  
Nanoscale Imaging ◽  
Nanoscale Features ◽  
Stimulated Raman

Stimulated Raman scattering (SRS) microscopy is an emerging technology that provides high chemical specificity for endogenous biomolecules and can circumvent common constraints of fluorescence microscopy including limited capabilities to probe small biomolecules and difficulty resolving many colors simultaneously due to spectral overlap. However, the resolution of SRS microscopy remains governed by the diffraction limit. To overcome this, we describe a new technique called Molecule Anchorable Gel-enabled Nanoscale Imaging of Fluorescence and stImulatEd Raman Scattering microscopy (MAGNIFIERS), that integrates SRS microscopy with expansion microscopy (ExM). ExM is a powerful strategy providing significant improvement in imaging resolution by physical magnification of hydrogel-embedded preserved biological specimens. MAGNIFIERS offers chemical-specific nanoscale imaging with sub-50 nm resolution and has scalable multiplexity when combined with multiplex Raman probes and fluorescent labels. We used MAGNIFIERS to visualize nanoscale features in a label-free manner with C-H vibration of proteins, lipids and DNA in a broad range of biological specimens, from mouse brain, liver and kidney to human lung organoid. In addition, we applied MAGNIFIERS to track nanoscale features of protein synthesis in protein aggregates using metabolic labeling of small metabolites. Finally, we used MAGNIFIERS to demonstrate 8-color nanoscale imaging in an expanded mouse brain section. Overall, MAGNIFIERS is a valuable platform for super-resolution label-free chemical imaging, high-resolution metabolic imaging, and highly multiplexed nanoscale imaging, thus bringing SRS to nanoscopy.

scholarly journals Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jianpeng Ao ◽  
Xiaofeng Fang ◽  
Xianchong Miao ◽  
Jiwei Ling ◽  
Hyunchul Kang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Design Principle ◽  
Stimulated Raman Scattering ◽  
Super Resolution ◽  
Single Molecule Spectroscopy ◽  
Multiplexed Imaging ◽  
Chemical Resolution ◽  
Super Resolution Microscopy ◽  
Stimulated Raman

AbstractPhotochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding “on” or “off” SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.

scholarly journals Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

Science ◽  
2008 ◽  
Vol 322 (5909) ◽  
pp. 1857-1861 ◽  
Author(s):  
Christian W. Freudiger ◽  
Wei Min ◽  
Brian G. Saar ◽  
Sijia Lu ◽  
Gary R. Holtom ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Biomedical Imaging ◽  
Stimulated Raman Scattering ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Raman Microscopy ◽  
Label Free ◽  
Omega 3 ◽  
Low Sensitivity ◽  
Stimulated Raman

Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis.

Selective suppression of CARS signal with three-beam competing stimulated Raman scattering processes

2018 ◽  
Vol 20 (25) ◽  
pp. 17156-17170 ◽  
Author(s):  
Dae Sik Choi ◽  
B. Jayachander Rao ◽  
Doyeon Kim ◽  
Sang-Hee Shim ◽  
Hanju Rhee ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Selective Suppression ◽  
Stimulated Raman

A switching-off of the CARS signal is achieved by using a three-beam double SRS scheme.

Selective Suppression of Stimulated Raman Scattering with Another Competing Stimulated Raman Scattering

2017 ◽  
Vol 8 (24) ◽  
pp. 6118-6123 ◽  
Author(s):  
Doyeon Kim ◽  
Dae Sik Choi ◽  
Jiwoong Kwon ◽  
Sang-Hee Shim ◽  
Hanju Rhee ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Selective Suppression ◽  
Stimulated Raman

Ultra-violet Stimulated Raman Scattering of SrWO4 Crystal

2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Ultra Violet ◽  
Stimulated Raman
Sign in / Sign up

Export Citation Format

Share Document