scholarly journals Rationalizing Inter- and Intracrystal Heterogeneities in Dealuminated Acid Mordenite Zeolites by Stimulated Raman Scattering Microscopy Correlated with Super-resolution Fluorescence Microscopy

ACS Nano ◽  
2014 ◽  
Vol 8 (12) ◽  
pp. 12650-12659 ◽  
Author(s):  
Kuan-Lin Liu ◽  
Alexey V. Kubarev ◽  
Jordi Van Loon ◽  
Hiroshi Uji-i ◽  
Dirk E. De Vos ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Super Resolution ◽  
Stimulated Raman

scholarly journals Super-resolution vibrational imaging using expansion stimulated Raman scattering microscopy

2021 ◽  
Author(s):  
Lixue Shi ◽  
Aleksandra Klimas ◽  
Brendan Gallagher ◽  
Zhangyu Cheng ◽  
Feifei Fu ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Mouse Brain ◽  
Stimulated Raman Scattering ◽  
Super Resolution ◽  
Chemical Imaging ◽  
Metabolic Imaging ◽  
Label Free ◽  
Nanoscale Imaging ◽  
Nanoscale Features ◽  
Stimulated Raman

Stimulated Raman scattering (SRS) microscopy is an emerging technology that provides high chemical specificity for endogenous biomolecules and can circumvent common constraints of fluorescence microscopy including limited capabilities to probe small biomolecules and difficulty resolving many colors simultaneously due to spectral overlap. However, the resolution of SRS microscopy remains governed by the diffraction limit. To overcome this, we describe a new technique called Molecule Anchorable Gel-enabled Nanoscale Imaging of Fluorescence and stImulatEd Raman Scattering microscopy (MAGNIFIERS), that integrates SRS microscopy with expansion microscopy (ExM). ExM is a powerful strategy providing significant improvement in imaging resolution by physical magnification of hydrogel-embedded preserved biological specimens. MAGNIFIERS offers chemical-specific nanoscale imaging with sub-50 nm resolution and has scalable multiplexity when combined with multiplex Raman probes and fluorescent labels. We used MAGNIFIERS to visualize nanoscale features in a label-free manner with C-H vibration of proteins, lipids and DNA in a broad range of biological specimens, from mouse brain, liver and kidney to human lung organoid. In addition, we applied MAGNIFIERS to track nanoscale features of protein synthesis in protein aggregates using metabolic labeling of small metabolites. Finally, we used MAGNIFIERS to demonstrate 8-color nanoscale imaging in an expanded mouse brain section. Overall, MAGNIFIERS is a valuable platform for super-resolution label-free chemical imaging, high-resolution metabolic imaging, and highly multiplexed nanoscale imaging, thus bringing SRS to nanoscopy.

scholarly journals Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jianpeng Ao ◽  
Xiaofeng Fang ◽  
Xianchong Miao ◽  
Jiwei Ling ◽  
Hyunchul Kang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Design Principle ◽  
Stimulated Raman Scattering ◽  
Super Resolution ◽  
Single Molecule Spectroscopy ◽  
Multiplexed Imaging ◽  
Chemical Resolution ◽  
Super Resolution Microscopy ◽  
Stimulated Raman

AbstractPhotochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding “on” or “off” SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.

Ultra-violet Stimulated Raman Scattering of SrWO4 Crystal

2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Ultra Violet ◽  
Stimulated Raman

Bulk Laser Damage Threshold and Stimulated Raman Scattering in KTiOPO4 Crystal.

1996 ◽  
Vol 24 (8) ◽  
pp. 906-909
Author(s):  
Akio MIYAMOTO ◽  
Hidetsugu YOSHIDA ◽  
Yusuke MORI ◽  
Takatomo SASAKI ◽  
Sadao NAKAI
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Damage Threshold ◽  
Laser Damage Threshold ◽  
Laser Damage ◽  
Stimulated Raman
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