Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy

2009 ◽  
Vol 121 (47) ◽  
pp. 9152-9156 ◽  
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

scholarly journals Inside Cover: Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy (Angew. Chem. Int. Ed. 47/2009)

2009 ◽  
Vol 48 (47) ◽  
pp. 8798-8798
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Innentitelbild: Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy (Angew. Chem. 47/2009)

2009 ◽  
Vol 121 (47) ◽  
pp. 8956-8956
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Label-Free Chemical Imaging of Catalytic Solids by Coherent Anti-Stokes Raman Scattering and Synchrotron-Based Infrared Microscopy

2009 ◽  
Vol 48 (47) ◽  
pp. 8990-8994 ◽  
Author(s):  
Marianne���H.���F. Kox ◽  
Katrin���F. Domke ◽  
James���P.���R. Day ◽  
Gianluca Rago ◽  
Eli Stavitski ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Chemical Imaging ◽  
Label Free ◽  
Infrared Microscopy ◽  
Anti Stokes

Tracking the Formation and Degradation of Fatty-Acid-Accumulated Mitochondria Using Label-Free Chemical Imaging

2020 ◽  
Author(s):  
Chi Zhang ◽  
Stephen Boppart
Keyword(s):  
Fatty Acid ◽  
Structural Features ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Dynamic Changes ◽  
Regulatory Processes ◽  
Transport Chain ◽  
Anti Stokes ◽  
First Time

Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited autofluorescence to study the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal NADH and FAD metabolism, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features, likely caused by the mitophagy process. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of this organelle under perturbative conditions.

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy

2014 ◽  
pp. 189-201 ◽  
Author(s):  
Antti Isomäki ◽  
Tarvo Sillat ◽  
Mari Ainola ◽  
Mikko Liljeström ◽  
Yrjö T. Konttinen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Label Free ◽  
Anti Stokes

scholarly journals Coherent anti-Stokes Raman scattering for label-free biomedical imaging

2013 ◽  
Vol 15 (9) ◽  
pp. 094006 ◽  
Author(s):  
Imran I Patel ◽  
Christian Steuwe ◽  
Stefanie Reichelt ◽  
Sumeet Mahajan
Keyword(s):  
Raman Scattering ◽  
Biomedical Imaging ◽  
Label Free ◽  
Anti Stokes

Label-Free Imaging of Lipophilic Bioactive Molecules during Lipid Digestion by Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy

2010 ◽  
Vol 132 (24) ◽  
pp. 8433-8439 ◽  
Author(s):  
James P. R. Day ◽  
Gianluca Rago ◽  
Katrin F. Domke ◽  
Krassimir P. Velikov ◽  
Mischa Bonn
Keyword(s):  
Raman Scattering ◽  
Bioactive Molecules ◽  
Label Free ◽  
Lipid Digestion ◽  
Anti Stokes
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