High-resolution stimulated Raman scattering microscopy for metabolites and specific protein imaging (Conference Presentation)

2019 ◽  
Author(s):  
Ping Wang
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Specific Protein ◽  
Protein Imaging ◽  
Stimulated Raman

High Resolution Wide Field Stimulated Raman Scattering Microscopy

2010 ◽  
Author(s):  
Yang-Hyo Kim ◽  
Daekeun Kim ◽  
Shyamsunder Erramilli ◽  
Peter T. C. So ◽  
P. M. Champion ◽  
...  
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Wide Field ◽  
Stimulated Raman

scholarly journals High Resolution Wide Field Stimulated Raman Scattering Microscopy

2010 ◽  
Vol 98 (3) ◽  
pp. 180a
Author(s):  
Yang-Hyo Kim ◽  
Daekeun Kim ◽  
Shyamsunder Erramilli ◽  
Peter T.C. So
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Wide Field ◽  
Stimulated Raman

scholarly journals Paper-Thin Multilayer Microfluidic Devices with Integrated Valves

2020 ◽  
Author(s):  
Soohong Kim ◽  
Gabriel Dorlhiac ◽  
Rodrigo Cotrim Chaves ◽  
Mansi Zalavadia ◽  
Aaron Streets
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Soft Lithography ◽  
Stimulated Raman Scattering ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Fluorescent Imaging ◽  
Cover Glass ◽  
On Chip ◽  
Stimulated Raman

Integrated valve microfluidics has an unparalleled capability to automate the rapid delivery of fluids at the nanoliter scale for high-throughput biological experimentation. However, multilayer soft lithography, which is used to fabricate valve-microfluidics, produces devices with a minimum thickness of around five millimeters. This form-factor limitation prevents the use of such devices in experiments with limited sample thickness tolerance such as 4-pi microscopy, stimulated Raman scattering microscopy, and many forms of optical or magnetic tweezer applications. We present a new generation of integrated valve microfluidic devices that are less than 300 μm thick, including the cover-glass substrate, that resolves the thickness limitation. This "thin-chip" was fabricated through a novel soft-lithography technique that produces on-chip micro-valves with the same functionality and reliability of traditional thick valve-microfluidic devices despite the orders of magnitude reduction in thickness. We demonstrated the advantage of using our thin-chip over traditional thick devices to automate fluid control while imaging on a high-resolution inverted microscope. First, we demonstrate that the thin-chip provides improved signal to noise when imaging single cells with two-color stimulated Raman scattering (SRS). We then demonstrated how the thin-chip can be used to simultaneously perform on-chip magnetic manipulation of beads and fluorescent imaging. This study reveals the potential of our thin-chip in high-resolution imaging, sorting, and bead capture-based single-cell multi-omics applications.

scholarly journals Live-cell Imaging Analysis of Antimycin-Type Depsipeptides via Bioorthogonal Stimulated Raman Scattering Microscopy

2019 ◽  
Author(s):  
Jeremy Seidel ◽  
Yupeng Miao ◽  
William Porterfield ◽  
Wenlong Cai ◽  
Xuejun Zhu ◽  
...  
Keyword(s):  
Natural Products ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Specific Protein ◽  
Imaging Analysis ◽  
Protein Targets ◽  
Distribution Study ◽  
Structure Activity ◽  
Anti Cancer ◽  
Stimulated Raman

AbstractSmall-molecule natural products have been an essential source of pharmaceuticals to treat human diseases, but very little is known about their behavior inside dynamic, living human cells. Here, we demonstrate the first structure-activity-distribution study of complex natural products, the anti-cancer antimycin-type depsipeptides, using the emerging bioorthogonal Stimulated Raman Scattering (SRS) Microscopy. Our results show that the intracellular enrichment and distribution of these compounds are driven by their potency and specific protein targets, as well as the lipophilic nature of compounds.

Ultra-violet Stimulated Raman Scattering of SrWO4 Crystal

2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Ultra Violet ◽  
Stimulated Raman

Bulk Laser Damage Threshold and Stimulated Raman Scattering in KTiOPO4 Crystal.

1996 ◽  
Vol 24 (8) ◽  
pp. 906-909
Author(s):  
Akio MIYAMOTO ◽  
Hidetsugu YOSHIDA ◽  
Yusuke MORI ◽  
Takatomo SASAKI ◽  
Sadao NAKAI
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Damage Threshold ◽  
Laser Damage Threshold ◽  
Laser Damage ◽  
Stimulated Raman
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