High Resolution Wide Field Stimulated Raman Scattering Microscopy

2010 ◽  
Author(s):  
Yang-Hyo Kim ◽  
Daekeun Kim ◽  
Shyamsunder Erramilli ◽  
Peter T. C. So ◽  
P. M. Champion ◽  
...  
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Wide Field ◽  
Stimulated Raman

scholarly journals High Resolution Wide Field Stimulated Raman Scattering Microscopy

2010 ◽  
Vol 98 (3) ◽  
pp. 180a
Author(s):  
Yang-Hyo Kim ◽  
Daekeun Kim ◽  
Shyamsunder Erramilli ◽  
Peter T.C. So
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Wide Field ◽  
Stimulated Raman

scholarly journals Paper-Thin Multilayer Microfluidic Devices with Integrated Valves

2020 ◽  
Author(s):  
Soohong Kim ◽  
Gabriel Dorlhiac ◽  
Rodrigo Cotrim Chaves ◽  
Mansi Zalavadia ◽  
Aaron Streets
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Soft Lithography ◽  
Stimulated Raman Scattering ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Fluorescent Imaging ◽  
Cover Glass ◽  
On Chip ◽  
Stimulated Raman

Integrated valve microfluidics has an unparalleled capability to automate the rapid delivery of fluids at the nanoliter scale for high-throughput biological experimentation. However, multilayer soft lithography, which is used to fabricate valve-microfluidics, produces devices with a minimum thickness of around five millimeters. This form-factor limitation prevents the use of such devices in experiments with limited sample thickness tolerance such as 4-pi microscopy, stimulated Raman scattering microscopy, and many forms of optical or magnetic tweezer applications. We present a new generation of integrated valve microfluidic devices that are less than 300 μm thick, including the cover-glass substrate, that resolves the thickness limitation. This "thin-chip" was fabricated through a novel soft-lithography technique that produces on-chip micro-valves with the same functionality and reliability of traditional thick valve-microfluidic devices despite the orders of magnitude reduction in thickness. We demonstrated the advantage of using our thin-chip over traditional thick devices to automate fluid control while imaging on a high-resolution inverted microscope. First, we demonstrate that the thin-chip provides improved signal to noise when imaging single cells with two-color stimulated Raman scattering (SRS). We then demonstrated how the thin-chip can be used to simultaneously perform on-chip magnetic manipulation of beads and fluorescent imaging. This study reveals the potential of our thin-chip in high-resolution imaging, sorting, and bead capture-based single-cell multi-omics applications.

Ultra-violet Stimulated Raman Scattering of SrWO4 Crystal

2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Ultra Violet ◽  
Stimulated Raman

Bulk Laser Damage Threshold and Stimulated Raman Scattering in KTiOPO4 Crystal.

1996 ◽  
Vol 24 (8) ◽  
pp. 906-909
Author(s):  
Akio MIYAMOTO ◽  
Hidetsugu YOSHIDA ◽  
Yusuke MORI ◽  
Takatomo SASAKI ◽  
Sadao NAKAI
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Damage Threshold ◽  
Laser Damage Threshold ◽  
Laser Damage ◽  
Stimulated Raman
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