Correlative multiphoton microscopy and infrared nanospectroscopy of label-free collagen

2019 ◽  
Author(s):  
Jérémie Mathurin ◽  
Gervaise Mosser ◽  
Alexandre Dazzi ◽  
Ariane Deniset-Besseau ◽  
Marie-Claire Schanne-Klein ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free

scholarly journals Label-free multiphoton microscopy reveals altered tissue architecture in hippocampal sclerosis

Epilepsia ◽  
2016 ◽  
Vol 58 (1) ◽  
pp. e1-e5 ◽  
Author(s):  
Ortrud Uckermann ◽  
Roberta Galli ◽  
Susann Leupold ◽  
Roland Coras ◽  
Matthias Meinhardt ◽  
...  
Keyword(s):  
Hippocampal Sclerosis ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Tissue Architecture

scholarly journals Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall

2014 ◽  
Vol 26 (5) ◽  
pp. 685-691
Author(s):  
Michal Sikora ◽  
David Scheiner ◽  
Cornelia Betschart ◽  
Daniele Perucchini ◽  
José María Mateos ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Multiphoton Microscopy ◽  
Three Dimensional ◽  
Vaginal Wall ◽  
Anterior Vaginal Wall ◽  
Label Free

Label-free Imaging of Gliomas with Multiphoton Microscopy

2017 ◽  
Author(s):  
Na Fang ◽  
Zanyi Wu ◽  
Xingfu Wang ◽  
Yuanxiang Lin ◽  
Lianhuang Li ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free

scholarly journals Diabetic Wounds Exhibit Distinct Microstructural and Metabolic Heterogeneity through Label-Free Multiphoton Microscopy

2016 ◽  
Vol 136 (1) ◽  
pp. 342-344 ◽  
Author(s):  
Kyle P. Quinn ◽  
Ermelindo C. Leal ◽  
Ana Tellechea ◽  
Antonios Kafanas ◽  
Michael E. Auster ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free ◽  
Metabolic Heterogeneity ◽  
Diabetic Wounds

scholarly journals Multiphoton imaging reveals axial differences in metabolic autofluorescence signals along the kidney proximal tubule

2018 ◽  
Vol 315 (6) ◽  
pp. F1613-F1625 ◽  
Author(s):  
Milica Bugarski ◽  
Joana Raquel Martins ◽  
Dominik Haenni ◽  
Andrew M. Hall
Keyword(s):  
Mitochondrial Function ◽  
Redox State ◽  
Kidney Diseases ◽  
Multiphoton Microscopy ◽  
Kidney Tissue ◽  
Kidney Cortex ◽  
Label Free ◽  
Multiphoton Imaging ◽  
Imaging Approach ◽  
Lactate And Pyruvate

Kidney proximal tubules (PTs) are densely packed with mitochondria, and defects in mitochondrial function are implicated in many kidney diseases. However, little is known about intrinsic mitochondrial function within PT cells. Here, using intravital multiphoton microscopy and live slices of mouse kidney cortex, we show that autofluorescence signals provide important functional readouts of redox state and substrate metabolism and that there are striking axial differences in signals along the PT. Mitochondrial NAD(P)H intensity was similar in both PT segment (S)1 and S2 and was sensitive to changes in respiratory chain (RC) redox state, whereas cytosolic NAD(P)H intensity was significantly higher in S2. Mitochondrial NAD(P)H increased in response to lactate and butyrate but decreased in response to glutamine and glutamate. Cytosolic NAD(P)H was sensitive to lactate and pyruvate and decreased dramatically in S2 in response to inhibition of glucose metabolism. Mitochondrial flavoprotein (FP) intensity was markedly higher in S2 than in S1 but was insensitive to changes in RC redox state. Mitochondrial FP signal increased in response to palmitate but decreased in response to glutamine and glutamate. Fluorescence lifetime decays were similar in both S1 and S2, suggesting that intensity differences are explained by differences in abundance of the same molecular species. Expression levels of known fluorescent mitochondrial FPs were higher in S2 than S1. In summary, substantial metabolic information can be obtained in kidney tissue using a label-free live imaging approach, and our findings suggest that metabolism is tailored to the specialized functions of S1 and S2 PT segments.

scholarly journals Label-free analysis of experimental lung fibrosis using multiphoton microscopy and Raman spectroscopy

2021 ◽  
Author(s):  
Ingo Ganzleben ◽  
Lucas Kreiß ◽  
Alexander Mühlberg ◽  
Oliver Friedrich ◽  
Markus F. Neurath ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Lung Fibrosis ◽  
Multiphoton Microscopy ◽  
Label Free
Sign in / Sign up

Export Citation Format

Share Document