Label-free detection of fibrillar collagen deposition associated with vascular elements in glioblastoma multiforme by using multiphoton microscopy

2016 ◽  
Vol 265 (2) ◽  
pp. 207-213 ◽  
Author(s):  
L.W. JIANG ◽  
X.F. WANG ◽  
Z.Y. WU ◽  
P.H. LIN ◽  
H.P. DU ◽  
...  
Keyword(s):  
Glioblastoma Multiforme ◽  
Multiphoton Microscopy ◽  
Fibrillar Collagen ◽  
Collagen Deposition ◽  
Label Free ◽  
Vascular Elements ◽  
Label Free Detection

scholarly journals An integrated multiomic and quantitative label-free microscopy-based approach to study pro-fibrotic signalling in ex vivo human precision-cut lung slices

2020 ◽  
pp. 2000221
Author(s):  
Muzamil Majid Khan ◽  
Daniel Poeckel ◽  
Aliaksandr Halavatyi ◽  
Joanna Zukowska-Kasprzyk ◽  
Frank Stein ◽  
...  
Keyword(s):  
Immune Cell ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Integrated Approach ◽  
Fibrillar Collagen ◽  
Collagen Deposition ◽  
Label Free ◽  
Human Lung Tissue ◽  
Ecm Proteins ◽  
Precision Cut Lung Slices

Fibrosis can affect any organ resulting in the loss of tissue architecture and function with often life-threatening consequences. Pathologically, fibrosis is characterised by expansion of connective tissue due to excessive deposition of extracellular matrix proteins (ECM), including the fibrillar forms of collagen. A significant limitation for discovering cures for fibrosis is the availability of suitable human models and techniques to quantify mature fibrillar collagen deposition as close as possible to human physiological conditions. Here we have extensively characterised an ex vivo cultured human lung tissue-derived, precision-cut lung slices model (hPCLS) using label-free second harmonic (SHG) light microscopy to quantify fibrillar collagen deposition and mass spectrometry-based techniques to obtain a proteomic and metabolomic fingerprint of hPCLS in ex vivo culture.We demonstrate that hPCLS are viable and metabolically active with mesenchymal, epithelial, endothelial, and immune cell types surviving for at least 2 weeks in ex vivo culture. Analysis of hPCLS-conditioned supernatants showed a strong induction of pulmonary fibrosis-related ECM proteins upon TGFß1 stimulation. This upregulation of ECM proteins was not translated into an increased deposition of fibrillar collagen. In support of this observation, we revealed the presence of a pro-ECM degradation activity in our ex vivo cultures of hPCLS, inhibition of which by metalloproteinase inhibitor resulted in increased collagen deposition in response to TGFß1 stimulation. Together the data show that an integrated approach of measuring soluble pro-fibrotic markers alongside quantitative SHG-based analysis of fibrillar collagen is a valuable tool for studying pro-fibrotic signalling and testing antifibrotic agents.

scholarly journals Rapid, label‐free detection of intracranial germinoma using multiphoton microscopy

2019 ◽  
Vol 6 (03) ◽  
pp. 1
Author(s):  
Na Fang ◽  
Zanyi Wu ◽  
Xingfu Wang ◽  
Ning Cao ◽  
Yuanxiang Lin ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free ◽  
Intracranial Germinoma ◽  
Label Free Detection

scholarly journals An advanced optical clearing protocol allows label-free detection of tissue necrosis via multiphoton microscopy in injured whole muscle

Theranostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 2876-2891
Author(s):  
Dominik Schneidereit ◽  
Anita Bröllochs ◽  
Paul Ritter ◽  
Lucas Kreiß ◽  
Zeinab Mokhtari ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free ◽  
Tissue Necrosis ◽  
Optical Clearing ◽  
Label Free Detection ◽  
Whole Muscle

scholarly journals Label-Free Detection of Breast Masses Using Multiphoton Microscopy

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65933 ◽  
Author(s):  
Xiufeng Wu ◽  
Gang Chen ◽  
Jianping Lu ◽  
Weifeng Zhu ◽  
Jingting Qiu ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free ◽  
Breast Masses ◽  
Label Free Detection

Label-free detection of residual breast cancer after neoadjuvant chemotherapy using biomedical multiphoton microscopy

2019 ◽  
Vol 34 (8) ◽  
pp. 1595-1601 ◽  
Author(s):  
Zhonghua Han ◽  
Lianhuang Li ◽  
Deyong Kang ◽  
Zhenlin Zhan ◽  
Haohua Tu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Neoadjuvant Chemotherapy ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Label Free Detection

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

2018 ◽  
Vol 16 (1) ◽  
pp. 015603 ◽  
Author(s):  
Na Fang ◽  
Zanyi Wu ◽  
Shanshan Cai ◽  
Yupeng Chen ◽  
Yuanxiang Lin ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free ◽  
Brain Invasion ◽  
Label Free Detection

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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