Near-infrared light therapy for recovery of cerebral hypoperfusion induced by bilateral common carotid artery stenosis in mice

2019 ◽  
Author(s):  
Dong-Jin Lee ◽  
Ha-Young Jang ◽  
Ki-Wook Moon ◽  
Eun-Joo Lee ◽  
A-Ram Yoo ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Common Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Near Infrared ◽  
Light Therapy ◽  
Artery Stenosis ◽  
Cerebral Hypoperfusion ◽  
Infrared Light ◽  
Near Infrared Light ◽  
Bilateral Common Carotid Artery

scholarly journals Sequential PET estimation of cerebral oxygen metabolism with spontaneous respiration of 15O-gas in mice with bilateral common carotid artery stenosis

2017 ◽  
Vol 37 (10) ◽  
pp. 3334-3343 ◽  
Author(s):  
Takashi Temma ◽  
Makoto Yamazaki ◽  
Jun Miyanohara ◽  
Hisashi Shirakawa ◽  
Naoya Kondo ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Common Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Oxygen Metabolism ◽  
Artery Stenosis ◽  
Spontaneous Respiration ◽  
Cerebral Oxygen ◽  
Cerebral Oxygen Metabolism ◽  
Bilateral Common Carotid Artery ◽  
Post Operation

Positron emission tomography with 15O-labeled gases (15O-PET) is important for in vivo measurement of cerebral oxygen metabolism both in clinical and basic settings. However, there are currently no reports concerning 15O-PET in mice. Here, we developed an 15O-PET method applicable to mice with spontaneous respiration of 15O-gas without a tracheotomy catheter. Sequential 15O-PET was also performed in a mouse model of chronic cerebral hypoperfusion with bilateral common carotid artery stenosis (BCAS) induced by placement of microcoils. 15O-gas with isoflurane was supplied to the nose of mouse with evacuation of excess 15O-gas surrounding the body. 15O-PET was performed on days 3, 7, 14, 21, and 28 after surgery. Cerebral blood flow (CBF), cerebral blood volume, oxygen extraction fraction (OEF), and cerebral metabolic rate of oxygen (CMRO2) were calculated in whole brains. A significant decrease in CBF and compensatory increase in OEF in the BCAS group produced CMRO2 values comparable to that of the sham group at three days post-operation. Although CBF and OEF in the BCAS group gradually recovered over the first 28 days, the CMRO2 showed a gradual decrease to 68% of sham values at 28 days post-operation. In conclusion, we successfully developed a noninvasive 15O-PET method for mice.

scholarly journals Cardiac Dysfunction in a Mouse Vascular Dementia Model of Bilateral Common Carotid Artery Stenosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Lulu An ◽  
Michael Chopp ◽  
Alex Zacharek ◽  
Yi Shen ◽  
Zhili Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cognitive Function ◽  
Carotid Artery ◽  
Vascular Dementia ◽  
Common Carotid Artery ◽  
Cardiac Dysfunction ◽  
Carotid Artery Stenosis ◽  
Artery Stenosis ◽  
Heart Weight ◽  
Bilateral Common Carotid Artery

Background: Cardiac function is associated with cognitive function. Previously, we found that stroke and traumatic brain injury evoke cardiac dysfunction in mice. In this study, we investigate whether bilateral common carotid artery stenosis (BCAS), a model that induces vascular dementia (VaD) in mice, induces cardiac dysfunction.Methods: Late-adult (6–8 months) C57BL/6J mice were subjected to sham surgery (n = 6) or BCAS (n = 8). BCAS was performed by applying microcoils (0.16 mm internal diameter) around both common carotid arteries. Cerebral blood flow and cognitive function tests were performed 21–28 days post-BCAS. Echocardiography was conducted in conscious mice 29 days after BCAS. Mice were sacrificed 30 days after BCAS. Heart tissues were isolated for immunohistochemical evaluation and real-time PCR assay.Results: Compared to sham mice, BCAS in mice significantly induced cerebral hypoperfusion and cognitive dysfunction, increased cardiac hypertrophy, as indicated by the increased heart weight and the ratio of heart weight/body weight, and induced cardiac dysfunction and left ventricular (LV) enlargement, indicated by a decreased LV ejection fraction (LVEF) and LV fractional shortening (LVFS), increased LV dimension (LVD), and increased LV mass. Cognitive deficits significantly correlated with cardiac deficits. BCAS mice also exhibited significantly increased cardiac fibrosis, increased oxidative stress, as indicated by 4-hydroxynonenal and NADPH oxidase-2, increased leukocyte and macrophage infiltration into the heart, and increased cardiac interleukin-6 and thrombin gene expression.Conclusions: BCAS in mice without primary cardiac disease provokes cardiac dysfunction, which, in part, may be mediated by increased inflammation and oxidative stress.

scholarly journals Near-Infrared Transillumination Back Scattering Sounding—New Method to Assess Brain Microcirculation in Patients with Chronic Carotid Artery Stenosis

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61936 ◽  
Author(s):  
Andrzej F. Frydrychowski ◽  
Pawel J. Winklewski ◽  
Arkadiusz Szarmach ◽  
Grzegorz Halena ◽  
Tomasz Bandurski
Keyword(s):  
Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Near Infrared ◽  
Artery Stenosis ◽  
New Method ◽  
Back Scattering ◽  
Brain Microcirculation

scholarly journals Diagnostic dilemmas of multislice CT angiography in the evaluation of the degree of common carotid artery stenosis

2010 ◽  
Vol 138 (7-8) ◽  
pp. 494-497
Author(s):  
Dragoslav Nenezic ◽  
Slobodan Tanaskovic ◽  
Predrag Gajin ◽  
Nenad Ilijevski ◽  
Goran Vucurevic
Keyword(s):  
Carotid Artery ◽  
Ct Angiography ◽  
Common Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Soft Tissues ◽  
Multislice Ct ◽  
Diagnostic Procedures ◽  
Left Common Carotid Artery ◽  
Artery Stenosis ◽  
High Grade

Introduction. Multislice CT angiography (CTA) is a noninvasive and quick technique to image carotid artery stenosis, as well as intracerebral vasculature. Modern multidetector CTA produces images with a high resolution of, not only the contrast-filled lumen, but also of the vessel wall and the surrounding soft tissues. Multiple studies have verified the ability of CTA to provide an accurate representation of the degree of carotid stenosis in comparison to digital subtraction angiography, both for moderate and high-grade stenosis. Because of its fast and accurate vessel imaging, CT angiography is increasingly used in the assessment of carotid artery stenosis. Case Outline. A 37-year-old female patient was admitted at the Vascular Surgery Clinic of the Institute for Cardiovascular Diseases 'Dedinje', Belgrade, for angiography and endovascular procedure of a high-grade stenosis of the left common carotid artery based on Multislice CT findings brought by the patient. She complained of problems which we considered to be the result of cerebral circulation ischemia. After detailed diagnostic procedures, we concluded that no pathological lesions could be verified either on the left common carotid artery or other supraaortic branches. Therefore, the patient was discharged for further neurological examinations. Conclusion. Although Multislice CTA has many advantages over classical angiography, its validity should be taken with reserve, especially in younger patients.

scholarly journals Longitudinal effects of common carotid artery stenosis on ocular hemodynamics assessed using laser speckle flowgraphy in a rabbit model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Aishah Ismail ◽  
Hui Cheng Chen ◽  
Ibrahima Faye ◽  
Tong Boon Tang
Keyword(s):  
Carotid Artery ◽  
Common Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Rabbit Model ◽  
Laser Speckle ◽  
Artery Stenosis ◽  
Laser Speckle Flowgraphy ◽  
Stroke Severity ◽  
Post Surgery ◽  
The Impact

Abstract Real-time impairment of ocular blood flow (OBF) under common carotid artery stenosis (CCAS) has not been ascertained. We aimed to longitudinally assess the impact of CCAS on OBF using a rabbit model. About 75% stenosis was created by tying the common carotid artery with a plastic mandrel using a nylon suture. The plastic mandrel was gently removed, leaving a ligature. Neurological and behavioral assessments were recorded as the clinical indicator of stroke severity. With laser speckle flowgraphy, the pulse waveform parameters namely mean blur rate (MBR), blowout score (BOS), blowout time (BOT), rising rate, S1-area, falling rate (FR), S2-area, flow acceleration index (FAI), acceleration time index, resistive index (RI) and the difference between the maximum and minimum values of MBR (AC) were assessed in overall, vessel, and tissue regions of the optic nerve head (ONH). Longitudinally, BOS significantly increased until day 19 post-surgery, whereas FAI, RI, and AC significantly decreased. Beyond day 19, BOS, BOT, FR, FAI, RI, and AC significantly decreased. We defined two stages representing impaired vessel conditions, namely the vessel resistance phase, where BOS increases and FAI, RI, and AC decrease, and the vessel elasticity phase where BOS, BOT, FR, FAI, RI and AC decrease. These stages provide information about atherosclerosis, assessable non-invasively through the eye.

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