Evaluation of photomedicine and anti-microRNA combination therapies in 3D cell culture models of pancreatic ductal adenocarcinoma (Conference Presentation)

2019 ◽  
Author(s):  
Ljubica Petrovic ◽  
Tomas Materdey ◽  
Eric Struth ◽  
Maud-Emmanuelle Gilles ◽  
Meirav Segal ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pancreatic Ductal Adenocarcinoma ◽  
3D Cell Culture ◽  
Ductal Adenocarcinoma ◽  
Combination Therapies ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Conjunctival melanoma and electrochemotherapy: preliminary results using 2D and 3D cell culture models in vitro

2018 ◽  
Vol 97 (4) ◽  
pp. e632-e640 ◽  
Author(s):  
Miltiadis Fiorentzis ◽  
Periklis Katopodis ◽  
Helen Kalirai ◽  
Berthold Seitz ◽  
Arne Viestenz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Conjunctival Melanoma ◽  
Preliminary Results ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

2010 ◽  
Vol 8 (11) ◽  
pp. 791-801 ◽  
Author(s):  
Jennifer Barrila ◽  
Andrea L. Radtke ◽  
Aurélie Crabbé ◽  
Shameema F. Sarker ◽  
Melissa M. Herbst-Kralovetz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Host Pathogen Interactions ◽  
Rotating Wall ◽  
Rotating Wall Vessel ◽  
Host Pathogen ◽  
Culture Models ◽  
Cell Culture Models

Length vs. stiffness: which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

2018 ◽  
Vol 6 (25) ◽  
pp. 4223-4231 ◽  
Author(s):  
Jiacheng Zhao ◽  
Hongxu Lu ◽  
Yin Yao ◽  
Sylvia Ganda ◽  
Martina H. Stenzel
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Dominant Role ◽  
3D Cell Culture ◽  
Culture Models ◽  
Nano Rods ◽  
2D And 3D ◽  
Cell Culture Models

Internalization of rod-like micelles by breast cancer cells is significantly affected by the stiffness of nano-rods.

scholarly journals Interstitial Flow Recapitulates Gemcitabine Chemoresistance in A 3D Microfluidic Pancreatic Ductal Adenocarcinoma Model by Induction of Multidrug Resistance Proteins

2019 ◽  
Vol 20 (18) ◽  
pp. 4647 ◽  
Author(s):  
Bart Kramer ◽  
Luuk de Haan ◽  
Marjolein Vermeer ◽  
Thomas Olivier ◽  
Thomas Hankemeier ◽  
...  
Keyword(s):  
Cell Culture ◽  
Multidrug Resistance ◽  
Pancreatic Ductal Adenocarcinoma ◽  
3D Cell Culture ◽  
In Vitro Models ◽  
Ductal Adenocarcinoma ◽  
Interstitial Flow ◽  
Multidrug Resistance Proteins ◽  
And Function

Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most lethal cancers due to a high chemoresistance and poor vascularization, which results in an ineffective systemic therapy. PDAC is characterized by a high intratumoral pressure, which is not captured by current 2D and 3D in vitro models. Here, we demonstrated a 3D microfluidic interstitial flow model to mimic the intratumoral pressure in PDAC. We found that subjecting the S2-028 PDAC cell line to interstitial flow inhibits the proliferation, while maintaining a high viability. We observed increased gemcitabine chemoresistance, with an almost nine-fold higher EC50 as compared to a monolayer culture (31 nM versus 277 nM), and an alleviated expression and function of the multidrug resistance protein (MRP) family. In conclusion, we developed a 3D cell culture modality for studying intratissue pressure and flow that exhibits more predictive capabilities than conventional 2D cell culture and is less time-consuming, and more scalable and accessible than animal models. This increase in microphysiological relevance might support improved efficiency in the drug development pipeline.

scholarly journals Untargeted Metabolite Mapping in 3D Cell Culture Models Using High Spectral Resolution FT-ICR Mass Spectrometry Imaging

2019 ◽  
Vol 91 (15) ◽  
pp. 9522-9529 ◽  
Author(s):  
Lulu H. Tucker ◽  
Gregory R. Hamm ◽  
Rebecca J. E. Sargeant ◽  
Richard J. A. Goodwin ◽  
C. Logan Mackay ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Spectral Resolution ◽  
Mass Spectrometry Imaging ◽  
3D Cell Culture ◽  
High Spectral Resolution ◽  
Culture Models ◽  
Cell Culture Models ◽  
Ft Icr
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